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| 一株西藏牦牛源多杀性巴氏杆菌基因分型及rpoE基因生物信息学分析 |
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周赛赛1,李天娇1,武琦1,朱家平1,罗宗1,吕然1,赵晓慧1,王一飞1,罗润波1,贡嘎1,王保宁1,2,索朗斯珠1*
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1.西藏农牧学院 动物科学学院, 西藏 林芝 860000;2.四川大学 华西基础医学与法医学院, 成都 610041
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| 摘要: |
| 为分析本实验室分离保存的一株西藏牦牛源多杀性巴氏杆菌基因型和rpoE基因编码蛋白的生物特性，通过16S rDNA、特异性基因、荚膜分型、脂多糖分型、rpoE基因扩增等分子生物学和生物信息学方法对此株牦牛源多杀性巴氏杆菌进行分析。结果表明:此株牦牛源多杀性巴氏杆菌为B∶L2∶ST44基因型，rpoE基因和印度牛源多杀性巴氏杆菌rpoE基因相似性为100%，分子量22.0 ku，理论等电点4.86，属于不稳定、无跨膜结构、无信号肽、有保守结构域和有潜在磷酸化的胞内亲水性蛋白，二级结构α-螺旋占比最高67.02%，三级结构为棒状微弯曲模型，存在多个抗原表位，预测可与10个蛋白存在相互作用。综上，本研究对一株西藏牦牛源多杀性巴氏杆菌进行基因分型和rpoE基因进行生物信息分析，为进一步了解西藏牦牛源多杀性巴氏杆菌基因型和探索RpoE蛋白的生物学特性提供数据参考。 |
| 关键词: 牦牛 多杀性巴氏杆菌 基因分型 rpoE基因 生物信息学分析 |
| DOI：10.11841/j.issn.1007-4333.2021.05.06 |
| 分类号: |
| 基金项目:国家肉牛牦牛产业体系项目(CARS-37)；西藏自治区科技厅2019年度重点项目 |
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| Genotyping and rpoE gene bioinformatics analysis of a Pasteurella multocida strain in Tibetan yaks |
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ZHOU Saisai1,LI Tianjiao1,WU Qi1,ZHU Jiaping1,LUO Zong1,LV Ran1,ZHAO Xiaohui1,WANG Yifei1,LUO Runbo1,GONG Ga1,WANG Baoning1,2,Suolangsizhu1*
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1.Department of Animal Science, Tibet Agricultural and Animal Husbandry College, Linzhi 860000, China;2.Department of Pathogenic Biology, West China School of Basic Medical Science and Forensic Medicine, Sichuan University, Chengdu 610041, China
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| Abstract: |
| In order to analyze the genotype of one Tibetan yak-derived Pasteurella multocida strain isolated and preserved in our laboratory and the biological characteristics of the protein encoded by the rpoE gene, 16S rDNA gene, specific gene. capsule typing, lipopolysaccharide typing, and rpoE gene amplification and other molecular biological and bioinformatics methods were used. The results showed that the genotype of the P. multocida strain from yak was B∶L2∶ST44. The similarity of rpoE gene between the isolate and that from Indian cattle was 100%. The molecular weight of the rpoE protein was 22. 0 ku and the theoretical pI was 4. 86. The protein was unstable without transmembrane structure and signal peptide, and had conservative domain and potential phosphorylation function for intracellular hydrophilic. The secondary structure of α-helix accounted for 67. 02%, the tertiary structure was rod-shaped micro bending model, there were multiple antigen epitopes, the predicted interaction with 10 proteins. In conclusion, this study conducted genotyping and bioinformatic analysis of rpoE gene of a P. multocida strain from Tibetan yaks, which provided references for further understanding the genotype of P. multocida from Tibetan yaks and exploring the biological characteristics of RpoE protein. |
| Key words: yak Pasteurella multocida genotyping rpoE gene bioinformatics analysis |
