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Normal and Premature Rupture of Fetal Membranes at Term Delivery Differ in Regional Chemotactic Activity and Related Chemokine/Cytokine Production

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Abstract

A gradient of immunological mediators exists in the fetal membranes from the periplacental zone (PZ) to the rupture zone (RZ) at term delivery (rupture of fetal membranes [ROM]). However, it is unknown if this gradient is different in premature rupture of these tissues (premature rupture of fetal membranes [PROM]). We therefore analyzed leukocyte chemotactic activity and chemokine/cytokine production in fetal membrane zones in ROM and PROM. In ROM, leukocyte chemotactic activity increased from the PZ to the RZ; however, this did not occur in PROM. This was due to consistently elevated leukocyte chemotactic activity in PROM compared to ROM tissues. In the RZ, ROM was characterized by increased T-cell attraction and high levels of chemokine (C-X-C motif) ligand 8 (CXCL-8)/interleukin 8, and PROM by increased granulocyte attraction and high levels of granulocyte-macrophage colony-stimulating factor and CXCL-10/interferon gamma-induced protein 10. We conclude that normal and premature rupture of fetal membranes differ in regional chemotactic activity and related chemokine/cytokine production, which may represent evidence for differential mechanisms of rupture at term delivery.

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Authors and Affiliations

  1. Research Direction, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico

    Nardhy Gomez-Lopez PhD

  2. Discipline of Obstetrics & Gynaecology and Robinson Institute, The University of Adelaide, Adelaide, SA, 5005, Australia

    Nardhy Gomez-Lopez PhD & Andrew P. Lobb BSc

  3. Departments of Obstetrics and Gynecology, Pediatrics & Physiology, University of Alberta, Edmonton, Alberta, Canada

    Nardhy Gomez-Lopez PhD & David M. Olson PhD

  4. Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico

    Susana Hernandez-Santiago BSc

  5. Biochemistry Department, Faculty of Medicine, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico

    Felipe Vadillo-Ortega MD, PhD

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  1. Nardhy Gomez-Lopez PhD
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  2. Susana Hernandez-Santiago BSc
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  5. Felipe Vadillo-Ortega MD, PhD
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Correspondence to Nardhy Gomez-Lopez PhD.

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Gomez-Lopez, N., Hernandez-Santiago, S., Lobb, A.P. et al. Normal and Premature Rupture of Fetal Membranes at Term Delivery Differ in Regional Chemotactic Activity and Related Chemokine/Cytokine Production. Reprod. Sci. 20, 276–284 (2013). https://doi.org/10.1177/1933719112452473

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