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Significant Correlation Between Maternal Body Mass Index at Delivery and in the Second Trimester, and Second Trimester Circulating Total Cell-free DNA Levels

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Abstract

Objectives

The use of cell-free fetal deoxyribonucleic acid (DNA) requires the knowledge of variables that may influence the levels of cell-free DNA, such as maternal body mass index (BMI).

Material and methods

In this study, using 406 maternal blood samples from the second trimester of pregnancy, cell-free fetal DNA specific for the SRY and DYS14 loci and glyceraldehyde-3-phosphate dehydrogenase sequence were quantified by real-time polymerase chain reaction.

Results

No significant correlation was seen between the levels of cell-free fetal DNA and maternal BMI, whereas total cell-free DNA was significantly associated with maternal BMI at 20 to 21 weeks of gestation (P = .034) and at the end of pregnancy (R2 regression: 0.016, P = .014).

Conclusion

Quantitative levels of cell-free fetal DNA are not affected by maternal BMI, whereas total DNA levels in the second trimester significantly correlate with maternal BMI at the moment of blood drawing and at delivery.

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Authors and Affiliations

  1. Departments of Obstetrics and Gynecology, and Research, University of Basel, Spitalstrasse 21, 4031, Basel, Switzerland

    Olav Lapaire MD, Simon Grill PhD, Irène Hösli MD, Rosanna Zanetti-Daellenbach MD, Xiao Yan Zhong MD & Wolfgang Holzgreve MD

  2. Medical Practice for Prenatal Medicine, Greifswald, Germany

    Thorsten Volgmann MD

Authors
  1. Olav Lapaire MD
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  2. Thorsten Volgmann MD
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  3. Simon Grill PhD
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  4. Irène Hösli MD
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  5. Rosanna Zanetti-Daellenbach MD
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  6. Xiao Yan Zhong MD
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  7. Wolfgang Holzgreve MD
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Corresponding author

Correspondence to Wolfgang Holzgreve MD.

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Lapaire, O., Volgmann, T., Grill, S. et al. Significant Correlation Between Maternal Body Mass Index at Delivery and in the Second Trimester, and Second Trimester Circulating Total Cell-free DNA Levels. Reprod. Sci. 16, 274–279 (2009). https://doi.org/10.1177/1933719108327599

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  • DOI: https://doi.org/10.1177/1933719108327599

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