Skip to main content
SpringerLink
Log in

Paternal Age Is Not Associated With Pregnancy Outcomes After Single Thawed Euploid Blastocyst Transfer

  • Original Article
  • Published:
Reproductive Sciences Aims and scope Submit manuscript

Abstract

Although controversial, increasing paternal age has been shown to negatively affect assisted reproductive technology (ART) outcomes and success rates. Most studies investigating the effect of paternal age on ART outcomes use a donor oocyte model to minimize maternal aneuploidy contribution. This study sought to determine whether increasing paternal age is associated with adverse in vitro fertilization (IVF) outcomes when aneuploidy is minimized using preimplantation genetic screening. There were 573 single thawed euploid embryo transfers from 473 patients undergoing oocyte donor and autologous IVF cycles. Cycles were categorized according to paternal age at oocyte retrieval, and an age adjustment was performed for maternal age in order to evaluate for an isolated paternal age effect. Fertilization rate was found to decrease significantly with increasing paternal age (P =.04). After controlling for oocyte age, there was no significant difference in pregnancy outcomes across all paternal age categories after euploid embryo transfer, including implantation rate (P =.23), clinical pregnancy rate (P =.51), and spontaneous abortion rate (P =.55). Therefore, if a couple is able to produce and transfer a single thawed euploid embryo, no difference in IVF pregnancy outcomes is identified with increasing paternal age.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bellver J, Garrido N, Remohi J, Pellicer A, Meseguer M. Influence of paternal age on assisted reproduction outcome. Reprod Biomed Online. 2008;17(5):595–604. doi:10.1016/S1472-6483(10)60305-7.

    Article  Google Scholar 

  2. Paulson RJ, Milligan RC, Sokol RZ. The lack of influence of age on male fertility. Am J Obstet Gynecol. 2001;184(5):818–822. doi: 10.1067/mob.2001.113852.

    Article  CAS  Google Scholar 

  3. de la Rochebrochard E, de Mouzon J, Thepot F, Thonneau P. French National IVFRA. Fathers over 40 and increased failure to conceive: the lessons of in vitro fertilization in France. Fertil Steril. 2006;85(5):1420–1424. doi:10.1016/j.fertnstert.2005.11. 040.

    Article  Google Scholar 

  4. Robertshaw I, Khoury J. Abdallah ME, Warikoo P, Hofmann GE. The effect of paternal age on outcome in assisted reproductive technology using the ovum donation model. Reprod Sci. 2014; 21(5):590–593. doi:10.1177/1933719113506497.

    Article  Google Scholar 

  5. Frattarelli JL, Miller K, Miller BT, Elkind-Hirsch K, Scott R. Jr. Male age negatively impacts embryo development and reproductive outcome in donor oocyte assisted reproductive technologies. Fertil Steril. 2008;90(1):97–103. doi:10.1016/j.fertnstert. 2007.06.009.

    Article  Google Scholar 

  6. Agarwal A, Said TM. Role of sperm chromatin abnormalitie, and DNA damage in male infertility. Hum Reprod Update. 2003;(9): 331–345. doi:10.1093/humupd/dmg027.

    Article  CAS  Google Scholar 

  7. Moskovtsev SI, Willis J, Mullen BM. Age-related decline in sperm deoxyribonucleic acid integrity in patients evaluated for male infertility. Fertil Steril. 2006;85(2):496–499. doi:10.1016/j.fertnstert.2005.05.075.

    Article  CAS  Google Scholar 

  8. Brahem S, Mehdi M, Elghezal H, Saad A. The effects of male aging on semen quality, sperm DNA. fragmentation and chromosomal abnormalities in an infertile population. J Assist Reprod Genet. 2011;28:425–432. doi:10.1007/s10815-011-9537-5.

    Article  Google Scholar 

  9. Rosenbusch BE. Cytogenetics of human spermatozoa: what about the reproductive relevance of structural chromosome aberrations? J Assist Reprod Genet. 1995;12(6):375–383. doi:10.1007/BF02215729.

    Article  CAS  Google Scholar 

  10. Sartorelli EM, Mazzucatto LF, de Pina-Neto JM. Effect of paternal age on human sperm chromosomes. Fertil Steril. 2001;76(6):1119–1123. doi:http://dx.doi.org/10.1016/S0015-0282(01)02894-1.

    Article  CAS  Google Scholar 

  11. Garcia-Ferrayra J, Luna D, Villegas L, et al. High aneuploidy rates observed in embryos derived from donated oocytes are related to male aging and high percentages of sperm DNA. fragmentation. Clin Med Insights Reprod Health. 2015;9:21–27. doi:10.4137/CMRH.S32769.

    Google Scholar 

  12. Vagnini L, Baruffi RL, Mauri AL, et al. The effects of male age of sperm DNA damage in an infertile population. Reprod Biomed Online. 2007;15:514–519. doi:10.1016/S1472-6483(10)60382-3.

    Article  CAS  Google Scholar 

  13. Lopes S, Jurisicova A, Sun JG, Casper RF. Reactive oxygen species: potential caus, for DNA fragmentation in human spermatozoa. Hum Reprod. 1998;13:896–900. PMID: 9619544.

    Article  CAS  Google Scholar 

  14. Morris ID, Ilott S, Dixon L, Brison DR. The spectru, of DNA damage in human sperm assessed by single cell gel electro-phoresis (Comet assay) and its relationship to fertilization and embryo development. Hum Reprod. 2002;17:990–998. PMID: 11925396.

    Article  CAS  Google Scholar 

  15. Gandini L, Lombardo F, Paoli D, et al. Full-term pregnancies achieved with ICSI despite high levels of sperm chromatin damage. Hum Reprod. 2004;19:1409–1417. doi:10.1093/humrep/ deh233.

    Article  CAS  Google Scholar 

  16. Luna M, Finkler E, Barritt J, et al. Paternal age and assisted reproductive technology outcome in ovum recipients. Fertil Steril. 2009; 92:1772–1775. doi:10.1016/j.fertnstert.2009.05.036.

    Article  Google Scholar 

  17. Braude P, Bolton V, Moore S. Human gene expression first occurs between the four- and eight-cell stages of preimplanta-tion development. Nature. 1988;332:459–461. doi:10.1038/ 332459a0.

    Article  CAS  Google Scholar 

  18. Duncan FE, Hornick JE, Lampson MA, Schultz RM, Shea LD, Woodruff TK. Chromosome cohesion decreases in human eggs with advanced maternal age. Aging Cell. 2012;11:1121–1124. doi:10.1111/j.1474-9726.2012.00866.x.

    Article  CAS  Google Scholar 

  19. Palermo G, Munné S, Cohen J. The human zygote inherits its mitotic potential from the male gamete. Hum Reprod. 1994;9: 1220–1225. PMID: 7962421.

    Article  CAS  Google Scholar 

  20. Kong A, Frigge ML, Masson G, et al. Rate of de novo mutations and the importance of father’s age to disease risk. Nature. 2012; 488:471–475. doi:10.1038/nature11396.

    Article  CAS  Google Scholar 

  21. Jenkins TG, Aston KI, Meyer T, Carrell DT. The sperm epigenome, male aging, and potential effects on the embryo. Adv Exp Med Biol. 2015:81–93. doi:10.1007/978-3-319-18881-2_4.

    Google Scholar 

  22. Sagi-Dain L, Sagi S, Dirnfeld M. Effect of paternal age on reproductive outcomes in oocyte donation model: a systematic review. Fertil Steril. 2015;104(4):857–865. doi:10.1016/j.fertnstert.2015. 06.036.

    Article  Google Scholar 

  23. Harton GL, Munne S, Surrey M, et al; PGD Practitioners Group. Diminished effect of maternal age on implantation after preim-plantation genetic diagnosis with array comparative genomic hybridization. Fertil Steril. 2013;100(6):1695–1703. doi:10. 1016/j.fertnstert.2013.07.2002.

    Article  CAS  Google Scholar 

  24. Grifo JA, Hodes-Wertz B, Lee HL, Amperloquio E, Clarke-Williams M, Adler A. Single thawed euploid embryo transfe, improves IV pregnancy, miscarriage, and multiple gestation outcomes and has similar implantation rates as egg donation. J Assist Reprod Genet. 2013;30(2):259–264. doi:10.1007/ s10815-012-9929-1.

    Article  Google Scholar 

  25. Gutiérrez-Mateo C, Colls P. Sánchez-Garcia J, et al. Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos. Fertil Steril. 2011;95: 953–958. doi:10.1016/j.fertnstert.2010.09.010.

    Article  Google Scholar 

  26. Haddad G, Deng M, Wang CT. et al. Assessment of aneuploidy formation in human blastocysts resulting from donated eggs and the necessity of the embryos for aneuploidy screening. J Assist Reprod Genet. 2015;31(6):999–1006. doi:10.1007/s10815-015-0492-4.

    Article  Google Scholar 

  27. Garcia-Velasco JA, Isaza V, Caligara C, Pellicer A, Remohi J, Simon C. Factors that determine discordant outcome from shared oocytes. Fertil Steril. 2003;80(1):54–60. doi:http://dx.doi.org/10.1016/S0015-0282(03)00545-4.

    Article  Google Scholar 

  28. Reig-Viader R, Capilla L, Vila-Cejudo M, et al. Telomere homeostasis is compromised in spermatocytes from patients with idio-pathic infertility. Fertil Steril. 2014;102(3):728–738. doi:10.1016/j.fertnstert.2014.06.005.

    Article  CAS  Google Scholar 

  29. Allsopp RC, Vaziri H, Patterson C, et al. Telomere length predicts replicative capacity of human fibroblasts. Proc Natl Acad Sci USA. 1992;89:10114–10118. PMID: 1438199.

    Article  CAS  Google Scholar 

  30. Aston KI, Hunt SC, Susser E, et al. Divergence of sperm and leukocyte age-dependent telomere dynamics: implications for male-driven evolution of telomere length in humans. Mol Hum Reprod. 2012;18:517–522. doi:10.1093/molehr/gas028.

    Article  CAS  Google Scholar 

  31. Baird DM, Britt-Compton B, Rowson J. Amso NN, Gregory L, Kipling D. Telomere instability in the male germline. Hum Mol Genet. 2006;15:45–51. doi:10.1093/hmg/ddi424.

    Article  CAS  Google Scholar 

  32. Atunes DMF, Kalmback KH, Wang F, et al. A single-cell assay for telomere DNA content shows increasing telomere length heterogeneity, as well as increasing mean telomere length in human spermatozoa with advancing age. J Assist Reprod Genet. 2015;32: 1685–1690. doi:10.1007/s10815-015-0574-3.

    Article  Google Scholar 

  33. Franasiak JM, Forman EJ, Hong KH, et al. The nature of aneu-ploidy with increasing age of the female partner: a review of 15,169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening. Fertil Steril. 2014;101(3):656–663. doi:10.1016/j.fertnstert.2013.11.004.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, New York University Langone Medical Center, New York, NY, USA

    Ashley W. Tiegs MD

  2. Department of Reproductive Endocrinology and Infertility, New York University Fertility Center, New York, NY, USA

    Nidhee M. Sachdev MD & Jamie A. Grifo MD, PhD

  3. Department of Obstetrics and Gynecology, New York University Fertility Center, New York, NY, USA

    Jamie A. Grifo MD, PhD & Frederick Licciardi MD

  4. Department of New York University Fertility Center, New York, NY, USA

    David H. McCulloh PhD, HCLD

  5. Department of Oocyte Donation Program, New York University Fertility Center, New York, NY, USA

    Frederick Licciardi MD

Authors
  1. Ashley W. Tiegs MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nidhee M. Sachdev MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jamie A. Grifo MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David H. McCulloh PhD, HCLD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Frederick Licciardi MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ashley W. Tiegs MD.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tiegs, A.W., Sachdev, N.M., Grifo, J.A. et al. Paternal Age Is Not Associated With Pregnancy Outcomes After Single Thawed Euploid Blastocyst Transfer. Reprod. Sci. 24, 1319–1324 (2017). https://doi.org/10.1177/1933719116687660

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1177/1933719116687660

Keywords

Search

Navigation