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Thrombospondin-1 and Thrombospondin-2 mRNA and TSP-1 and TSP-2 Protein Expression in Uterine Fibroids and Correlation to the Genes COL1A1 and COL3A1 and to the Collagen Cross-link Hydroxyproline

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Abstract

Uterine fibroids are composed of altered collagen fibrils and represent an arrested response to injury-initiating fibrosis. In many tissues, TSP-1 is secreted by adult macrophages and monocytes upon wounding and is involved in the activation of transforming growth factor β. In the absence of TSP-1, the orchestrated process of wound healing is impaired. The authors obtained tissue from the edge and center of fibroids at the time of hysterectomy and compared them with adjacent myometrium. The pattern of TSP-1 and TSP-2 expression was correlated to that of COL1A1 and COL3A1. Collagen and hydroxyproline were increased in fibroids. Thrombospondin-1 was consistently underexpressed in both the edge and center of the fibroids, while COL1A1 and COL3A1 were consistently overexpressed. However, TSP-2 was inconsistently expressed. These findings lead to the conclusion that the underexpression of TSP-1 may contribute to the overall development of uterine fibroids.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Duke University Medical Center, Box 3279, Durham, NC, 27710, USA

    Millie A. Behera MD, Liping Feng MD, Bryan Yonish BA & Phyllis C. Leppert MD, PhD

  2. Department of Obstetrics and Gynecology, Uniformed Services University, Bethesda, Maryland, USA

    William Catherino MD, PhD

  3. Department of Biostatistics and Bioinformatics, Duke University, Durham, North Carolina, USA

    Sin-Ho Jung PhD

  4. Reproductive Biology and Medicine Branch, National Institute of Child Health and Human Development, Bethesda, Maryland, USA

    Phyllis C. Leppert MD, PhD

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  1. Millie A. Behera MD
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  2. Liping Feng MD
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Correspondence to Phyllis C. Leppert MD, PhD.

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Funded by the National Institutes of Health 1 UL1-RR 024128 and the Department of Obstetrics and Gynecology Duke University Research Fund for New Faculty. Presented in part at the Eighth International Conference on the Extracellular Matrix of the Female Reproductive Tract, November 11 and 13, 2006, and at the Society for Gynecological Investigation Annual Meeting, March 14 to 17, 2007.

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Behera, M.A., Feng, L., Yonish, B. et al. Thrombospondin-1 and Thrombospondin-2 mRNA and TSP-1 and TSP-2 Protein Expression in Uterine Fibroids and Correlation to the Genes COL1A1 and COL3A1 and to the Collagen Cross-link Hydroxyproline. Reprod. Sci. 14 (Suppl 8), 63–76 (2007). https://doi.org/10.1177/1933719107309591

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