Symmetric and Asymmetric Hyoscine Membrane Sensor for Determination of Hyoscine Butyl Bromide in Pharmaceutical Formulation and Biological Fluids; A Computational Study

In this study, symmetric and asymmetric potentiometric liquid membrane sensors for simple and fast determination of hyoscine n-butyl bromide in pharmaceutical formulation and biological fluid were constructed. For the membrane preparation, hyoscine n-butyl-tetraphenyl borate ion-pair were employed as electroactive material of the membrane sensor. The wide linear range (10⁻⁵–10⁻¹ for symmetric and 10⁻⁷–10⁻³ for asymmetric sensor), low detection limit (3 g/mL for symmetric and 0.03 g/mL for asymmetric senor), and fast response time (15 and 10 s, respectively) are characterizations of the proposed sensors. Validation of the method shows suitability of the sensors for applies in the quality control measurement of hyoscine n-butyl bromide. The proposed method was found to be simple, accurate and precise in comparison with HPLC method and in cases which purification is required, the proposed sensor can be used as a detector for HPLC. Also, the electronic and geometric properties of hyoscine n-butyl bromide, sodium tetraphenyl borate and their complexes were studied by computational methods. The agreement mutually verifies the accuracy of experimental method and the validity of computational calculations.