Physical Factors Affecting Kinesin-Based Transport of Synthetic Nanoparticle Cargo

Recently, kinesin biomolecular motors and microtubules filaments (MTs) were used to transport metal and semiconductor nanoparticles with the long-term goal of exploiting this active transport system to dynamically assemble nanostructured materials. In some cases, however, the presence of nanoparticle cargo on MTs was shown to inhibit transport by interfering with kinesin-MT interactions. The primary objectives of this work were (1) to determine what factors affect the ability of kinesin and MTs to transport nanoparticle cargo, and (2) to establish a functional parameter space in which kinesin and MTs can support unimpeded transport of nanoparticles and materials. Of the factors evaluated, nanoparticle density on a given MT was the most significant factor affecting kinesin-based transport of nanoparticles. The density of particles was controlled by limiting the number of available linkage sites (i.e., biotinylated tubulin), and/or the relative concentration of nanoparticles in solution. Nanoparticle size was also a significant factor affecting transport, and attributed to the ability of particles ≤40 nm in diameter to bind to the "underside" of the MT, and block kinesin transport. Overall, a generalized method of assembling and transporting a range of nanoparticle cargo using kinesin and MTs was established.