Optimization of Holocellulolytic Enzymes Production by Cladosporium Cladosporioides Using Taguchi-L'16 Orthogonal Array

The aim of present study was to statistically optimize the influential parameters for the production of holocellulases from a newly isolated strain, Cladosporium cladosporioides under submerged cultivation system. Holocellulases (cellulases and xylanases) are the enzymatic mixtures which act on plant cell wall structural carbohydrate i.e., cellulose and hemicellulose so called holocellulose. Different fermentation parameters such as carbon source, nitrogen source, inorganic compounds like KH₂PO₄, MgSO₄ and pH of the medium were investigated at the individual and interactive level by Taguchi orthogonal array methodology. All selected fermentation parameters influenced the enzymes production. Rice straw, the major carbon source influenced the production of xylanases [48%] and cellulases [50%] at the individual level. KH₂PO₄ and MgSO₄ accounted for more than 54% of the severity index [SI] for xylanase interactively. Peptone in conjunction with pH accounted for nearly 41% of SI for cellulase production. After media optimization, a significant improvement in the yield of holocellulolytic enzymes was recorded ranging from 52 to 106 IU/ml [208%] for xylanase activity, 1.8 to 2.8 IU/ml for carboxymethyl cellulase (CMCase) [158%] and 2.0 to 2.9 IU/ml for filter paperase (FPase activity) [147%] indicating, the commercial potential of cellulolytic enzymes production by C. cladosporioides. Efficient holocellulosic degrading enzymatic cocktail plays a significant role in commercialization of biorefinery, textile, detergent formulation and paper manufacturing industries.