Comorbidity of sickle cell trait and albinism: a cross-sectional survey in the Democratic Republic of the Congo

Introduction Sickle Cell Disease (SCD) and albinism are both recessive hereditary diseases in human kind with a high prevalence in sub-Saharan Africa. This study aimed to determinate the prevalence of sickle cell trait in people living with albinism (PLA). Methods a cross-sectional descriptive survey was conducted in PLA attending the "Hôpital du Cinquantenaire de Kisangani". In total, by non-probabilistic convenience sampling, 82 albinos and 139 non-albinos and without any antecedents of albinism in their family were included, selected from students in the Faculty of Medicine and Pharmacy at the University of Kisangani. Blood samples were collected on "dried blood spot" and analyzed by mass spectrometry at CHU of Liège. Data were entered into an Excel file and analysed on SPSS 20.0 (Chicago, IL). Results forty-six of the 82 albinos (56.1%) were female and 43.9% male with a sex ratio of 1.28. Among albinos, 18.3% had hemoglobin AS (HbAS) and 81.7% hemoglobin AA (HbAA) compared to 18% of subjects with hemoglobin AS and 82% hemoglobin AA in the control group. The difference was not statistically significant (Chi-square=0.003, ddl=1, p=0.9544). Conclusion this study highlighted that the prevalence of the sickle cell trait is high among people living with albinism, but does not differ from that observed in non-albinos in the Democratic Republic of the Congo. It is therefore important to raise awareness among this category of people about sickle cell disease and the importance of its premarital screening.


Introduction
Sickle cell anemia and albinism are both recessive hereditary diseases in humans with a high prevalence in sub-Saharan Africa populations [1]. Today´s, sickle cell disease (SCD) is the most common genetic disease worldwide. The annual number of newborns with sickle cell disease was estimated to be 305 800 (CI: 238 400-398 800) in 2010 with 79% occurring in sub-Saharan Africa [2]. SCD is an autosomal recessive genetic disease, except in rare forms such as HbSAntilles and HbSOman, due to a mutation at the 6 th codon of the β-globin gene that leads to the replacement of adenine by thymine It has been estimated that albinism affects 1 in 20000 people worldwide; in Africa, incidences range from 1 in 2700 to 1 in 10000 people [6]. Oculocutaneous albinism is the most common type in sub-Saharan Africa with estimated prevalences of 1 in 1755 in Namibia, 1 in 2675 in Tanzania, 1 in 4728 in Zimbabwe and 1 in 3900 in South Africa. The overall prevalence of albinism in sub-Saharan Africa is estimated at 1 in 2000-5000 [7,8,12,13]. Oculocutaneous albinism, which is an autosomal recessive hereditary disease has been documented to co-exist with abnormal hemoglobin conditions like sickle cell disease [1]. But surveys that have focused on this co-morbidity are scarce in sub-Saharan Africa and the two studies that reported this observation, each involved only one albino family [14,15]. In the DRC, albinism is known to be widespread, nevertheless there have been no prevalence studies. Similarly, the prevalence of sickle cell disease is high, estimated at about 1.4% for the homozygous form [16] and 23.3% for the heterozygous form [17].
In addition, WHO estimates that the AS carrier rate is 25% and the annual incidence of the homozygous SS form around 15‰ births in the DRC [18], which is one of the three most affected countries by SCD worldwide [2]. However, to our knowledge, no studies evaluating the comorbidity of these two diseases have not yet been conducted.
This study aimed to determinate the prevalence of sickle cell trait in people living with albinism in Kisangani (northeastern of Democratic Republic of the Congo).

Methods
We conducted a cross-sectional descriptive survey in PLA attending the "Hôpital du Cinquantenaire de Kisangani" from the 30 th July to the 6 th September 2019, as well as among students in the Faculty of Medicine and Pharmacy at the University of Kisangani at the same period. In total, by non-probabilistic convenience sampling, we included 82 albinos and 139 non-albinos who constituted the control group. A total of 121 albinos visited the facility during the study period, but only 82 subjects who provided written consent or parental written consent for children, were included in the survey. Of all the students present at the time of the survey, 157 had willingness to provide written consent, but only 139 were included based on the inclusion criteria that were: not to be an albino and not having the history of albinism in the family. For haemoglobin phenotyping, blood samples were collected on "dried blood spot" (DBS) by capillary puncture and the analysis was performed by mass spectrometry at the Biochemical Genetics Laboratory of CHU of Liège. Data were entered into an Excel file and analyzed on SPSS 20.0 (Chicago, IL). In addition, the data were summarized in form of proportions and frequency tables for categorical variables. Continuous variables were summarized using means, median and standard deviation. P-value was computed for categorical variables using Chi-square (χ 2 ) test. A p-value of less than 0.05 was considered to constitute a statistically significant difference.

Results
Of the 221 subjects studied, 82 (37.1%) were albinos ranging in age from 1.6 to 48 years. Their mean age was 18

Discussion
In this study, among albinos, females represented 56.1% (Table 1) with a female to male ratio of 1,28; 18.3% had hemoglobin AS (HbAS) and 81.7% hemoglobin AA(HbAA) compared to 18% of subjects with hemoglobin AS and 82% hemoglobin AA in the control group but with no statistically significant difference (p-value >0.05) ( Table 2). disease. In all three cases, it was oculocutaneous albinism type 2 (OCA2) with a homozygous 2.7-kb deletion of the P gene. In one case, oculocutaneous albinism type 2 (OCA2) was associated with the homozygous form of sickle cell disease and in the other two cases, OCA2 was associated with the sickle cell trait. However, the study remained silent on a possible link between the two diseases [15].
In a more recent study, on the basis of empirical observation that

Conclusion
Our results show that the prevalence of the sickle cell trait is high among people living with albinism, but does not differ from that observed in non-albinos in the Democratic Republic of the Congo. It is therefore important to raise awareness among people living with albinism about sickle cell disease and the importance of its premarital screening, as well as the general population. The relatively small number of albinos is a limitation of this study. The same applies to the selection of the control group made by convenience sampling.
Nevertheless, this study contributes to the improvement of knowledge on the comorbidity sickle cell disease and albinism.
What is known about this topic • It has been suggested that there is no absolute linkage between sickle cell disease and albinism, but that a looser linkage was possible.

What this study adds
• This study provides data on the comorbidity of sickle cell disease and albinism in sub-Saharan Africa; • It also provides arguments that the prevalence of the sickle cell trait is not higher in people living with albinism, especially in the Democratic Republic of the Congo.

Competing interests
The authors declare no competing interests.

Authors' contributions
Kambale Kombi Paul designed the study, developed the protocol,  Tables   Table 1: characteristics of the participants