DEVELOPMENT OF OXIDATIVE STRESS AT ADRENALINE DAMAGE OF HEART IN MALE RATS WITH CASTRATION AND HYPODYNAMY

Abstract

Introduction. In recent months, due to the war in Ukraine, the number of people who are in a sedentary position and stressed for a long time has increased. The leading link in the development of any pathology is the development of oxidative stress, which affects the development of disorders of the cardiovascular system.

The aim of the study – to evaluate the effect of castration and hypodynamia on the development of oxidative processes in rats with epinephrine damage of heart (EDH).

Research Methods. Experiments were performed on 120 outbred male Wistar rats. All animals were divided into two series: 1 – control, 2 – castration and stress. Castration was performed under sodium thiopental anesthesia according to the method of Ya. D. Kirshenblatt. Hypodynamic stress was caused from 1.5 to 3 months by keeping them in cages with a limitation of the living space twice for 1.5 months. To reproduce EDH, rats were injected once intraperitoneally with a 0.18 % solution of adrenaline hydrotartrate at the rate of 0.5 mg/kg of weight (Pharmaceutical company "Darnytsia", Ukraine). The animals in each group were examined in control, after 1, 3, 7, 14 and 28 days. Under thiopental-sodium anesthesia, a blood sample was taken, where diene and triene conjugates (DC, TC), Schiff's bases (SB), TBA-active products (TBA-ap), superoxide dismutase (SOD), catalase activity (Cat), ceruloplasmin (CP).

Results and Discussion. In animals that underwent castration and stress, lower values of DC, TC, SB, SOD and CP, but higher TBA-ap and Cat. In rats of the 1st series, with the development of EDH, a greater increase in DC, TC, TBA-ap was noted up to 14 days, and SB decreased after 7 days, with the greatest increase in antioxidants after 7–14 days. In the 2nd series of rats, the maximum values of DC and TC were after 14 days, TBA-ap – after 1 day, SB – after 7 days, with the greatest increase of SOD after 14 days, and Cat and CP – after 1 day.

Conclusion. The combination of castration and stress in male rats causes a decrease in the products of lipid peroxide oxidation and the activity of superoxide dismutase and catalase and the content of ceruloplasmin. The development of EDH caused an increase in the products of lipid peroxidation, which was more pronounced in the control series of animals. Antioxidant activity increased maximally after 14 days in the control series of rats, and after 1 day in animals that underwent castration and stress.