Identification and characterization of a 2,2-dichloropropionic acid (2,2-DCP) degrading alkalotorelant bacterium strain BHS1 isolated from Blue Lake, Turkey

Authors

  • Batool Hazim Abdul Wahhab Department of Microbiology Faculty of Medicine Al-Mustansiriyah University, Iraq Department of Biosciences Universiti Teknologi Malaysia 81310 Johor Bahru, Malaysia
  • Nurul Fatin Syamimi Khairul Anuar Department of Biosciences Universiti Teknologi Malaysia 81310 Johor Bahru, Malaysia
  • Roswanira Abdul Wahab Department of Chemistry Universiti Teknologi Malaysia 81310 Johor Bahru, Malaysia
  • Marwan S.M. Al Nimer Department of Pharmacology Faculty of Pharmacy University Medical Hawler, Erbil, Iraq
  • Nurul HIdayah Samsulrizal Department of Plant Science, International Islamic University Malaysia 25200 Kuantan, Pahang, Malaysia
  • Azzmer Azzar Abdul Hamid Department of Biotechnology, International Islamic University Malaysia 25200 Kuantan, Pahang, Malaysia
  • Mohamed Faraj Edbeib Department of Animal Production Faculty of Agriculture Bani Walid University, Bani Walid, Libya
  • Yilmaz Kaya Department of Biology Faculty of Science Kyrgyz-Turkish Manas University, Bishkek, Turkey
  • Fahrul Huyop Department of Biosciences Universiti Teknologi Malaysia 81310 Johor Bahru, Malaysia

DOI:

https://doi.org/10.11594/jtls.10.03.08

Keywords:

Biodegradation, Bacillus, 2, 2-dichloropropionic acid, pollutant degradation, alkaline water, alkalotolerance, alkaliphiles

Abstract

An acid, 2,2-dichloropropionic acid (2,2-DCP) is an active ingredient in herbicide (Dalapon®). Using 2,2-DCP as a model substrate, an alkalotolerant bacterium was successfully isolated from the Blue Lake, Turkey. This bacterium is a potential bioremediation agent of recalcitrant xenobiotic halogenated compounds. This study aimed to prove the efficacy of the alkalotolerance Bacillus megaterium BHS1 in degrading 2,2-DCP as the sole source of carbon. Biolog GEN III system and 16S rRNA analysis were used for the identification of the bacterium. It was discovered that the strain BHS1 is Bacillus megaterium, and the bacterium that was observed to thrive in alkaline conditions (pH 7.0−14.0), supplemented with varying concentrations of 2,2-DCP (from 20 to 60 mM). Growth of strain BHS1 was exceptional in 40 mM of 2,2-DCP at pH 9, corresponding to a cell doubling time of 17.7 hour, whereas was fully inhibited at 50 mM 2,2-DCP. Since halogenated pollutants can make their way into highly alkaline environments, therefore, identifying threshold levels of strain BHS1 with respect to alkaline-tolerance and maximum level of 2,2-DCP may prove pertinent. This is to ensure that an optimal environment is created for the bacteria to degrade 2,2-DCP-contaminated water. In addition, this is the first study exploring a Bacillus species isolated from an alkaline environment adept in utilizing 2,2-DCP as a sole source of carbon. Hence, the ability of this strain to degrade other types of haloalkanoic acids constitutes a worthy future study.

References

Ismail SNF, Adamu A, F A, Huyop F, Shah A, Edbeib M, & Wahab R (2018) Dehalogenase producing bacteria from extreme environment: A review. Malaysian Journal of Microbiology 14(5):424-434.

Häggblom M, Knight V, & Kerkhof L (2000) Anaerobic decomposition of halogenated aromatic compounds. Environmental Pollution 107(2):199-207.

Ismail SNF, Adamu A, Aliyu F, Huyop F, SHAH A, Edbeib M, & Wahab R (2018) Dehalogenase producing bacteria from extreme environment: A review. Malaysian journal of microbiology 14(5):424-434.

Slonczewski JL, Fujisawa M, Dopson M, & Krulwich TA (2009) Cytoplasmic pH measurement and homeostasis in bacteria and archaea. Advances in microbial physiology 55:1-317.

Mesbah NM (2008) Anaerobic halophilic alkalithermophiles. (uga).

Abdulgader Edbeib MF, Wahab RA, & Huyop F (2016) Characterization of an α-haloalkanoic acid–degrading Pseudomonas aeruginosa MX1 isolated from contaminated seawater. Bioremediation Journal 20(2):89-97.

Slater JH, Bull AT, & Hardman DJ (1995) Microbial dehalogenation. Biodegradation 6(3):181-189.

Banciu HL & Muntyan MS (2015) Adaptive strategies in the double-extremophilic prokaryotes inhabiting soda lakes. Current opinion in microbiology 25:73-79.

Chinnathambi A (2015) Industrial important enzymes from alkaliphiles–an overview. Biosciences Biotechnology Research Asia 12(3):2007-2016.

Horikoshi K (1999) Alkaliphiles: some applications of their products for biotechnology. Microbiol. Mol. Biol. Rev. 63(4):735-750.

Guffanti AA, Finkelthal O, Hicks DB, Falk L, Sidhu A, Garro A, & Krulwich T (1986) Isolation and characterization of new facultatively alkalophilic strains of Bacillus species. Journal of bacteriology 167(3):766-773.

Krulwich TA & Guffanti AA (1989) Alkalophilic bacteria. Annual review of microbiology 43(1):435-463.

Sorokin DY (2011) The microbial sulfur cycle at extremely haloalkaline conditions of soda lakes. Frontiers in microbiology 2:44.

Yumoto I, Hirota K, Goto T, Nodasaka Y, & Nakajima K (2005) Bacillus oshimensis sp. nov., a moderately halophilic, non-motile alkaliphile. International journal of systematic and evolutionary microbiology 55(2):907-911

Xiong J, Liu Y, Lin X, Zhang H, Zeng J, Hou J, Yang Y, Yao T, Knight R, & Chu H (2012) Geographic distance and pH drive bacterial distribution in alkaline lake sediments across Tibetan Plateau. Environmental microbiology 14(9):2457-2466.

Ang LT, Tan AKY, Autio MI, Goh SH, Choo SH, Lee KL, Tan J, Pan B, Lee JJH, & Lum JJ (2018) A roadmap for human liver differentiation from pluripotent stem cells. Cell reports 22(8):2190-2205.

Akcay K & Kaya Y (2019) Isolation, characterization and molecular identification of a halotolerant Bacillus megaterium CTBmeg1 able to grow on halogenated compounds. Biotechnology & Biotechnological Equipment 33(1):945-953.

Khosrowabadi E & Huyop F (2014) Screening and characterization of several 2, 2-dicholoropropionic acid–degrading bacteria isolated from marine sediment of Danga Bay and east coast of Singapore island. Bioremediation journal 18(1):20-27.

Jing NH & Huyop F (2008) Enzymatic dehalogenation of 2, 2-dichloropropionic acid by locally isolated Methylobacterium sp. HJ1. Journal of Biological Science 8:233-235.

Wong W-Y & Huyop F (2011) Characterization of a Labrys sp. strain Wy1 able to utilize 2, 2-dichloropropionate (2, 2-DCP) as sole source of carbon. African Journal of Microbiology Research 5(20):3282-3288.

Hareland WA, Crawford RL, Chapman PJ, & Dagley S (1975) Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans. Journal of Bacteriology 121(1):272-285.

Bergmann J & Sanik J (1957) Determination of trace amounts of chlorine in naphtha. Analytical Chemistry 29(2):241-243.

Wragg P, Randall L, & Whatmore A (2014) Comparison of Biolog GEN III MicroStation semi-automated bacterial identification system with matrix-assisted laser desorption ionization-time of flight mass spectrometry and 16S ribosomal RNA gene sequencing for the identification of bacteria of veterinary interest. Journal of Microbiological Methods 105:16-21.

Johnson SL, Daligault HE, Davenport KW, Jaissle J, Frey KG, Ladner JT, Broomall SM, Bishop-Lilly KA, Bruce DC, & Gibbons HS (2015) Complete genome sequences for 35 biothreat assay-relevant Bacillus species. Genome Announc. 3(2):151-115.

Shivaji S, Chaturvedi P, Begum Z, Pindi PK, Manorama R, Padmanaban DA, Shouche YS, Pawar S, Vaishampayan P, & Dutt C (2009) Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere. International journal of systematic and evolutionary microbiology 59(12):2977-2986.

Heyrman J, Logan NA, Rodríguez-Díaz M, Scheldeman P, Lebbe L, Swings J, Heyndrickx M, & De Vos P (2005) Study of mural painting isolates, leading to the transfer of ‘Bacillus maroccanus’ and ‘Bacillus carotarum’to Bacillus simplex, emended description of Bacillus simplex, re-examination of the strains previously attributed to ‘Bacillus macroides’ and description of Bacillus muralis sp. nov. International journal of systematic and evolutionary microbiology 55(1):119-131.

Xi J, He L-Y, Huang Z, & Sheng X-F (2014) Bacillusqingshengii sp. nov., a rock-weathering bacterium isolated from weathered rock surface. International journal of systematic and evolutionary microbiology 64(7):2473-2479.

Chandna P, Mayilraj S, & Kuhad RC (2013) Bacillus paraflexus sp. nov., isolated from compost. International journal of systematic and evolutionary microbiology 63(12):4735-4743.

Wang H-l, Zhang J, & Sun L (2017) Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin. International journal of systematic and evolutionary microbiology 67(9):3547-3552.

Ten LN, Baek S-H, Im W-T, Larina LL, Lee J-S, Oh H-M, & Lee S-T (2007) Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field. International journal of systematic and evolutionary microbiology 57(11):2532-2537.

Tuo L, Liu F, Yan X-R, & Liu Y (2020) Bacillus taxi sp. nov., a novel endophytic bacterium isolated from root of Taxus chinensis (Pilger) Rehd. International Journal of Systematic and Evolutionary Microbiology 70(1):481-486.

Salim MM, Roslan D, & Huyop F (2011) Molecular analysis of dehalogenase gene in genomic DNA of Bacillus megaterium strain GS1 isolated from volcanic area Gunung Sibayak. Journal of Biological Sciences 11(5):394-398.

Sorokin DY, Berben T, Melton ED, Overmars L, Vavourakis CD, & Muyzer G (2014) Microbial diversity and biogeochemical cycling in soda lakes. Extremophiles 18(5):791-809.

Krulwich TA, Ito M, Hicks DB, Gilmour R, & Guffanti AA (1998) pH homeostasis and ATP synthesis: studies of two processes that necessitate inward proton translocation in extremely alkaliphilic Bacillus species. Extremophiles 2(3):217-222.

Borkar S (2015) Alkaliphilic Bacteria: Diversity, Physiology and Industrial Applications. Bioprospects of Coastal Eubacteria: Ecosystems of Goa:59.

Wu S, Zhang H, Hu Z, & Chen J (2009) Gene cloning and overexpression of dichloromethane dehalogenase from Bacillus circulans WZ-12. Huan jing ke xue= Huanjing kexue 30(8):2479-2484.

Zulkifly A, Roslan D, Hamid A, Hamdan S, & Huyop F (2010) Biodegradation of Low Concentration of Monochloroacetic Acid-Degrading Bacillus sp. TW 1 Isolated from Terengganu Water Treatment and Distribution Plant. Journal of Applied Sciences(Faisalabad) 10(22):2940-2944.

Gargaud M, William M, Ricardo A, Henderson James Jim C, Daniele L, José Cernicharo Q, Daniel R, Tilman S, Stéphane T, & Michel V (2015) Encyclopedia of astrobiology (Springer-Verlag Berlin Heidelberg, 2015).

Aono R & Horikoshi K (1983) Chemical composition of cell walls of alkalophilic strains of Bacillus. Microbiology 129(4):1083-1087.

Edbeib MF, Wahab RA, Huyop FZ, Aksoy HM, & Kaya Y (2020) Further Analysis of Burkholderia pseudomallei MF2 and Identification of Putative Dehalogenase Gene by PCR. Indonesian Journal of Chemistry 2020, 20 (2), 386 – 394.

Ismail SNF, Wahab RA, & Huyop F (2016) Isolation and identification of bacteria isolated from ruminant animal waste that able to degrade 2, 2-dichloropropionic acid (2, 2-DCP). Malaysian Journal of Microbiology 12(2):155-163.

Abel E, Ibrahim N, & Huyop F (2012) Identification of Serratia marcescens SE1 and determination of its herbicide 2, 2-dichloropropionate (2, 2-DCP) degradation potential. Malaysian Journal of Microbiology 8(4):259-265.

Mulyawati AI, Huyop F, Adamu A, Aliyu F, & Mustafa I (2018) Analysis of 2,2-DCP degrading bacteria isolated from a paddy field at a rural area in Malang, Indonesia. . Malaysian Journal of Microbiology 14(7):674-679.

Ghoreishi G, Alemzadeh A, Mojarrad M, & Djavaheri M (2017) Bioremediation capability and characterization of bacteria isolated from petroleum contaminated soils in Iran. Sustainable Environment Research 27(4):195-202.

Slater JH, Bull AT, & Hardman DJ (1996) Microbial dehalogenation of halogenated alkanoic acids, alcohols and alkanes. Advances in microbial physiology, (Elsevier), Vol 38, pp 133-176.

Kai S, Matsuo Y, Nakagawa S, Kryukov K, Matsukawa S, Tanaka H, Iwai T, Imanishi T, & Hirota K (2019) Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinIONâ„¢ nanopore sequencer. FEBS open bio 9(3):548-557.

Duckworth A, Grant W, Jones B, & Van Steenbergen R (1996) Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiology Ecology 19(3):181-191.

Sultanpuram VR, Mothe T, Chintalapati S, & Chintalapati VR (2017) Bacillus catenulatus sp. nov., an alkalitolerant bacterium isolated from a soda lake. Archives of microbiology 199(10):1391-1397.

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Published

2020-09-21

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Vol. 10 No. 3 (2020)

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