Supplementary Figure S2 from Heterochromatin Protein HP1γ Promotes Colorectal Cancer Progression and Is Regulated by miR-30a

Liu, Ming; Huang, Feifei; Zhang, Dan; Ju, Junyi; Wu, Xiao-Bin; Wang, Ying; Wang, Yadong; Wu, Yupeng; Nie, Min; Li, Zhuchen; Ma, Chi; Chen, Xi; Zhou, Jin-Yong; Tan, Renxiang; Yang, Bo-Lin; Zen, Ke; Zhang, Chen-Yu; Chen, Yu-Gen; Zhao, Quan

doi:10.1158/0008-5472.22406487.v1

00085472can143735-sup-142772_2_supp_0_nt3dhr.pdf (120.12 kB)

Supplementary Figure S2 from Heterochromatin Protein HP1γ Promotes Colorectal Cancer Progression and Is Regulated by miR-30a

journal contribution

posted on 2023-03-30, 23:20 authored by Ming Liu, Feifei Huang, Dan Zhang, Junyi Ju, Xiao-Bin Wu, Ying Wang, Yadong Wang, Yupeng Wu, Min Nie, Zhuchen Li, Chi Ma, Xi Chen, Jin-Yong Zhou, Renxiang Tan, Bo-Lin Yang, Ke Zen, Chen-Yu Zhang, Yu-Gen Chen, Quan Zhao

Effects of miR-30a on cell cycle and apoptosis.

History

ARTICLE ABSTRACT

Colorectal cancer pathogenesis remains incompletely understood. Here, we report that the heterochromatin protein HP1γ is upregulated commonly in human colorectal cancer, where it promotes cell proliferation in vitro and in vivo. Gene-expression and promoter-binding experiments demonstrated that HP1γ directly regulated CDKN1A (p21Waf1/Cip1) in a manner associated with methylation of histone H3K9 on its promoter. We identified miR-30a as a tumor-suppressive microRNA that targets HP1γ in vitro and in vivo to specifically suppress the growth of colorectal cancer in mouse xenograft models. MiR-30a was widely downregulated in primary human colorectal cancer tissues, where its expression correlated inversely with high levels of HP1γ protein. Our results identify a new miR-30a/HP1γ/p21 regulatory axis controlling colorectal cancer development, which may offer prognostic and therapeutic opportunities. Cancer Res; 75(21); 4593–604. ©2015 AACR.