00085472can133663-sup-tab1.xls (378.5 kB)

Supplementary Table 1 from EWS–WT1 Oncoprotein Activates Neuronal Reprogramming Factor ASCL1 and Promotes Neural Differentiation

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posted on 2023-03-30, 22:46 authored by Hong-Jun Kang, Jun Hong Park, WeiPing Chen, Soo Im Kang, Krzysztof Moroz, Marc Ladanyi, Sean Bong Lee

XLSX file - 378K, GO analysis of E-KTS and E+KTS induced genes in MEFs.

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ARTICLE ABSTRACT

The oncogenic fusion gene EWS–WT1 is the defining chromosomal translocation in desmoplastic small round-cell tumors (DSRCT), a rare but aggressive soft tissue sarcoma with a high rate of mortality. EWS–WT1 functions as an aberrant transcription factor that drives tumorigenesis, but the mechanistic basis for its pathogenic activity is not well understood. To address this question, we created a transgenic mouse strain that permits physiologic expression of EWS–WT1 under the native murine Ews promoter. EWS–WT1 expression led to a dramatic induction of many neuronal genes in embryonic fibroblasts and primary DSRCT, most notably the neural reprogramming factor ASCL1. Mechanistic analyses demonstrated that EWS–WT1 directly bound the proximal promoter of ASCL1, activating its transcription through multiple WT1-responsive elements. Conversely, EWS–WT1 silencing in DSRCT cells reduced ASCL1 expression and cell viability. Notably, exposure of DSRCT cells to neuronal induction media increased neural gene expression and induced neurite-like projections, both of which were abrogated by silencing EWS–WT1. Taken together, our findings reveal that EWS–WT1 can activate neural gene expression and direct partial neural differentiation via ASCL1, suggesting agents that promote neural differentiation might offer a novel therapeutic approach to treat DSRCT. Cancer Res; 74(16); 4526–35. ©2014 AACR.