Supplementary Figure 2 from A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer

Li, Ning; Kaur, Sippy; Greshock, Joel; Lassus, Heini; Zhong, Xiaomin; Wang, Yanling; Leminen, Arto; Shao, Zhongjun; Hu, Xiaowen; Liang, Shun; Katsaros, Dionyssios; Huang, Qihong; Bützow, Ralf; Weber, Barbara L.; Coukos, George; Zhang, Lin

doi:10.1158/0008-5472.22392653.v1

00085472can112484-sup-figure_s2_pdf_964k.pdf (964.68 kB)

Supplementary Figure 2 from A Combined Array-Based Comparative Genomic Hybridization and Functional Library Screening Approach Identifies mir-30d As an Oncomir in Cancer

journal contribution

posted on 2023-03-30, 21:08 authored by Ning Li, Sippy Kaur, Joel Greshock, Heini Lassus, Xiaomin Zhong, Yanling Wang, Arto Leminen, Zhongjun Shao, Xiaowen Hu, Shun Liang, Dionyssios Katsaros, Qihong Huang, Ralf Bützow, Barbara L. Weber, George Coukos, Lin Zhang

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ARTICLE ABSTRACT

Oncomirs are microRNAs (miRNA) that acts as oncogenes or tumor suppressor genes. Efficient identification of oncomirs remains a challenge. Here we report a novel, clinically guided genetic screening approach for the identification of oncomirs, identifying mir-30d through this strategy. mir-30d regulates tumor cell proliferation, apoptosis, senescence, and migration. The chromosomal locus harboring mir-30d was amplified in more than 30% of multiple types of human solid tumors (n = 1,283). Importantly, higher levels of mir-30d expression were associated significantly with poor clinical outcomes in ovarian cancer patients (n = 330, P = 0.0016). Mechanistic investigations suggested that mir-30d regulates a large number of cancer-associated genes, including the apoptotic caspase CASP3. The guided genetic screening approach validated by this study offers a powerful tool to identify oncomirs that may have utility as biomarkers or targets for drug development. Cancer Res; 72(1); 154–64. ©2011 AACR.