Supplementary Figure 2 from Genetically Engineered Salmonella typhimurium as an Imageable Therapeutic Probe for Cancer

Nguyen, Vu H.; Kim, Hyung-Seok; Ha, Jung-Min; Hong, Yeongjin; Choy, Hyon E.; Min, Jung-Joon

doi:10.1158/0008-5472.22382903.v1

00085472can093453-sup-sfig_2.pdf (1.28 MB)

Supplementary Figure 2 from Genetically Engineered Salmonella typhimurium as an Imageable Therapeutic Probe for Cancer

journal contribution

posted on 2023-03-30, 19:29 authored by Vu H. Nguyen, Hyung-Seok Kim, Jung-Min Ha, Yeongjin Hong, Hyon E. Choy, Jung-Joon Min

Supplementary Figure 2 from Genetically Engineered Salmonella typhimurium as an Imageable Therapeutic Probe for Cancer

History

ARTICLE ABSTRACT

Tumor-targeting bacteria have been studied in terms of their ability to visualize the infection pathway (through imaging probes) or to carry therapeutic molecules to tumors. To integrate these monitoring and therapeutic functions, we engineered attenuated Salmonella typhimurium defective in guanosine 5′-diphosphate-3′-diphosphate synthesis to carry cytotoxic proteins (cytolysin A) and express reporter genes. We successfully visualized the therapeutic process with these engineered bacteria in mice and found that they often mediated complete tumor (CT-26) eradication on cytotoxic gene induction. Furthermore, treatment with the engineered bacteria markedly suppressed metastatic tumor growth. Cancer Res; 70(1); 18–23