Supplementary Figure Legends 1-4 from Vitamin E Analogues Inhibit Angiogenesis by Selective Induction of Apoptosis in Proliferating Endothelial Cells: The Role of Oxidative Stress

Dong, Lan-Feng; Swettenham, Emma; Eliasson, Johanna; Wang, Xiu-Fang; Gold, Mikhal; Medunic, Yasmine; Stantic, Marina; Low, Pauline; Prochazka, Lubomir; Witting, Paul K.; Turanek, Jaroslav; Akporiaye, Emmanuel T.; Ralph, Stephen J.; Neuzil, Jiri

doi:10.1158/0008-5472.22371573.v1

00085472can073034-sup-legend_to_supplementary_figures_1-4.pdf (23.08 kB)

Supplementary Figure Legends 1-4 from Vitamin E Analogues Inhibit Angiogenesis by Selective Induction of Apoptosis in Proliferating Endothelial Cells: The Role of Oxidative Stress

journal contribution

posted on 2023-03-30, 17:50 authored by Lan-Feng Dong, Emma Swettenham, Johanna Eliasson, Xiu-Fang Wang, Mikhal Gold, Yasmine Medunic, Marina Stantic, Pauline Low, Lubomir Prochazka, Paul K. Witting, Jaroslav Turanek, Emmanuel T. Akporiaye, Stephen J. Ralph, Jiri Neuzil

Supplementary Figure Legends 1-4 from Vitamin E Analogues Inhibit Angiogenesis by Selective Induction of Apoptosis in Proliferating Endothelial Cells: The Role of Oxidative Stress

History

ARTICLE ABSTRACT

“Mitocans” from the vitamin E group of selective anticancer drugs, α-tocopheryl succinate (α-TOS) and its ether analogue α-TEA, triggered apoptosis in proliferating but not arrested endothelial cells. Angiogenic endothelial cells exposed to the vitamin E analogues, unlike their arrested counterparts, readily accumulated reactive oxygen species (ROS) by interfering with the mitochondrial redox chain and activating the intrinsic apoptotic pathway. The vitamin E analogues inhibited angiogenesis in vitro as assessed using the “wound-healing” and “tube-forming” models. Endothelial cells deficient in mitochondrial DNA (mtDNA) were resistant to the vitamin E analogues, both in ROS accumulation and apoptosis induction, maintaining their angiogenic potential. α-TOS inhibited angiogenesis in a mouse cancer model, as documented by ultrasound imaging. We conclude that vitamin E analogues selectively kill angiogenic endothelial cells, suppressing tumor growth, which has intriguing clinical implications. [Cancer Res 2007;67(24):11906–13]