Estimation of Genetic Parameters of Different Wheat Genotype Traits in Chitwan, Nepal

This research was carried out to estimate the genetic parameters of ﬁfty wheat genotypes received from the Agriculture Botany Division, Nepal Agriculture Research Council (NARC), Khumaltar, Lalitpur, in the winter season of 2014/2015 at Agriculture and Forestry University (AFU), Chitwan, Nepal. The experiment was performed in an alpha lattice design with two replications in ﬁve blocks in each replication and ten plots in each block. The analysis of variance showed highly signiﬁcant diﬀerences among the genotypes for all the studied traits except spike length. This implies that, except for spike length, all other traits exhibited genetic variability. The phenotypic coeﬃcient of variation (PCV) was generally higher than the genotypic coeﬃcient of variation (GCV) for all characters. The diﬀerence between PCV and GCV was large in spike length followed by grain weight per spike, the number of grains per spike, grain yield, thousand-grain weight, and harvest index indicating that these traits are aﬀected by the environment as well. Moderate to high heritability associated with a high genetic gain was observed for days to ﬂag leaf appearance, panicle length, thousand-grain weight, grain yield, and harvest index indicating the involvement of additive gene action. Grain weight per spike, number of grains per spike, thousand-grain weight, biological yield, and harvest index were signiﬁcantly correlated with grain yield indicating their important contribution to grain yield. WK 2525, WK 2437, and WK 2506 were three promising genotypes in terms of grain yield.


Introduction
Wheat (Triticum aestivum L.) is the staple food and the most widely grown cereal crop in the world with 717.2 million mt planted in 2013/2014 [1]. Wheat fulfills 21% of the total calories and 20% of the protein requirements of more than 4.5 billion people in 94 developing countries [2]. World wheat production is based almost entirely on two modern species: common or hexaploid bread wheat (Triticum aestivum, 2n � 6x � 42, AABBDD) and durum or tetraploid wheat (T. turgidum subsp. durum, 2n � 4x � 28, AABB). In Nepal, wheat is the third staple food after rice and maize in terms of production area, production, and productivity [3]. It is grown in the winter season in Nepal and is cultivated from the Terai (100masl) to a high mountain (2300 masl). At present, the area under wheat production in Nepal is 7,54,243 ha with a total production of 17,27,346 mt and productivity of 2.29 t ha −1 [3]. In Chitwan, wheat is cultivated on a total area of 8750 ha with a total production of 30,125 mt and productivity of 3.44 t ha −1 [3]. Mirza et al. [4] concluded that an improvement of 35 to 50% in wheat yield was achieved by the introduction of new high yielding cultivars in the country. It is the genetic makeup of a variety that is expressed by a favorable environment and produces different yields in different environments. Selections of improved and high yielding genotypes of different cereals with a wide range of adaptation to agroclimatic conditions are essential to increase yield per hectare.
Knowledge of the genetic variability present in existing crop species for the character under improvement is of paramount importance for the success of any plant breeding program. e observed variability is the result of the combined action of genetic and environmental factors. Among these two factors, only genetic factors were heritable. erefore, it is necessary to know the extent to which genetic factors contribute to the performance of a trait. erefore, the estimation of genetic parameters is important. Heredity with genetic advance is more helpful in predicting gain under effective selection [5]. us, the estimation of genetic parameters such as heritability and genetic advance can provide essential knowledge that can be decisive in predicting the transfer of traits from parental plants to offspring. PCV and GCV are other important genetic parameters. is gives an idea of whether the trait is environmentally affected or not. If the values of PCV and GCV are closer to each other or their difference is very small, it suggests that the trait is mainly due to the genetic makeup of the genotype. e greater the difference between the values of PCV and GCV, the greater the influence of the environment on the performance of the genotype. It is a very important parameter that helps to determine the influence of the environment on the performance of the genotype. Heritability and genetic advance are other important selection parameters that help plant breeders in determining the characters for which selection would be performed. Keeping in view the above perspectives, the present investigation was taken up to find out to evaluate the genotypes for yield and yield components for their performance, to estimate and analyze the genetic parameters like heritability and genetic advance present for quantitative and yield attributing traits in the wheat genotypes, and to find out the correlation among various traits present in wheat genotypes.

Research Site.
e experiment was conducted at the research farm of the Agriculture and Forestry University, Nepal, from November 2014 to June 2015. e global positioning system (GPS) of the experimental site was 27.64728°N, 84.34775°E; 27.64715°N, 84.34779°E and at an altitude of 228 m above sea level. In the experimental field with sandy loam soil texture, the pH was 6.4. e weather data of the experiment site are shown in Figure 1.

Plant Materials.
e experimental material was collected from the Agri Botany Division NARC, Khumaltar.
ere were 45 advanced wheat genotypes including five local check varieties (WK 1204, Chyakhura #1, Danphe, Munal #1, and WK 1481) from NARC for the study. Genotypes used in the research along with their source genotypes are presented in Table 1:

Experimental Details.
ere were 50 genotypes in the alpha lattice design in 2 replications with a plot size of 3 m × 1 m. Each replication comprised five blocks each consisting of ten plots. Each plot had 6 rows spaced 25 cm apart. Two deep ploughings followed by planking were done for field preparation. Well-rotted farmyard manure at a rate of 10 t ha −1 was applied at the time of field preparation. e dose of chemical fertilizers applied was 100 : 60 : 40 kg NPK per hectare. e basal dose of NPK was the half dose of nitrogen, the full dose of phosphorus, and potassium. e remaining dose of nitrogen was applied at the flowering and grain filling stage. Planting was performed on 3 rd December 2014. Irrigation was provided at the crown root initiation (CRI), flowering, and grain filling stages. Manual weeding was performed twice after 30 and 60 days of sowing. Harvesting was performed on 4 th April 2015. Each plot was harvested separately, threshed, well dried, and stored properly.

Data
Collection. Ten random plants per genotype per replication were used to record data. Observations were taken for the following quantitative traits: flag leaf area (cm), plant height (cm), peduncle length (cm), days to 50% flag leaf appearance, days to 50% booting, days to 50% heading, and days to 50% anthesis. Similarly, observations for yield attributing traits were taken for spike length (cm), the number of grains per spike, grain weight per spike (g), 1000 grain weight (g) days to physiological maturity, and grain yield (t ha −1 ) after moisture adjustment at 14% was taken. e total weight of biomass from each plot was weighed as grams per plot and then converted into kg per hectare to calculate the biological yield. Harvest index was calculated by the formula shown in e genotypic and phenotypic components of variance were computed according to formulae given by Lush [6] and Choudhary and Prasad [7] for the observed characteristics.
Genotypic variance (σ 2 g) � (TMSS -EMSS)/R, error variance � σ 2 e, and phenotypic variance (σ 2 p) � σ 2 g + σ 2 e, where TMSS is the treatment mean sum of squares, EMSS is the error mean sum of squares, and R is the number of replications. e genotypic and phenotypic coefficients of variation were computed according to Burton and Devane [8] and expressed as a percentage.
Genotypic coefficient of variation (GCV) � (σ g /X) x 100 and phenotypic coefficient of variation (PCV) � (σ p /X) x 100, where σ g is the genotypic standard deviation, σ p is the phenotypic standard deviation, and X is the general mean of character.

Broad Sense heritability (h 2 bs).
Broad sense heritability was estimated as the ratio of genotypic variance to phenotypic variance and expressed as a percentage [10].

Genetic Advance.
e extent of genetic advance to be expected by selecting 5% of the superior progeny was calculated using the following formula given by Robinson et al. [11].
GA � iσ p h 2, where i is the efficacy of selection which is 2.06 at 5% selection intensity, σ p is the phenotypic standard deviation, and h 2 is the heritability in a broad sense.

Genetic Advance as Percent of Mean (GAM).
GAM � (GA/X) x 100, where GA is the genetic advance, and X is the general mean of character.

Phenological Traits.
e mean number of days to flag leaf appearance was 63. e minimum number of days to flag leaf appearance was 50 DAS which was exhibited by WK 2495 whereas the maximum number of days to flag leaf appearance was exhibited by WK 2513 whose flag leaf appeared at 92 DAS. e mean number of days to booting was 69. e minimum day to booting was 57 DAS which was exhibited by WK 2495 whereas the maximum day to booting was exhibited by WK 2513 whose flag leaf appeared at 100 DAS. e mean number of days to heading was 75. e minimum day to heading was 65 DAS which was exhibited by WK 2495 whereas the maximum day to heading was exhibited by WK 2513 at 110 DAS. e mean number of days to anthesis was 80. e minimum day to anthesis was 71 DAS which was exhibited by WK 2495 whereas the maximum day to anthesis was exhibited by WK 2513 at 115 DAS. e mean number of days to maturity was 112. e minimum day to maturity was 101 DAS which was exhibited by WK 2353 whereas the maximum day to maturity was exhibited by WK 2513 at 138 DAS.

Growth Traits.
e mean flag leaf area was 55.75 cm 2 . e minimum flag leaf area was 26.33 cm 2 which was exhibited by WK 2505 whereas the maximum day to maturity was exhibited by WK 2515 at 103.64 cm 2 . e mean plant height was 98.5 cm. WK 2540 was the shortest genotype with a plant height of 77.25 cm whereas WK 1481 was the tallest genotype with a plant height of 125.95 cm. e mean peduncle length was 40.814 cm. WK 2437 was the genotype with the shortest peduncle length, i.e., 31 cm, whereas WK 2278 was the genotype with the longest peduncle length of 54.3 cm.

Yield and Yield Attributes.
e mean values of yield and yield attributing traits are presented in Table 2. e mean spike length was 9.91 cm. WK 2359 had the shortest spike length with a spike length of 7.2 cm, whereas WK 2555 had the longest spike length of 17.9 cm. e mean number of grains per spike was 36. WK 2550 had the highest number of grains per spike with 53 grains per spike whereas WK 2513 had the lowest number of grains per spike with 10 grains per spike. e mean grain weight per spike was 7.67 g. WK 2512 had the highest grain weight per spike with 21.3 g whereas WK 2513 had the lowest grain weight per spike with 0.745 g. e mean thousand-grain weight was 40.886 g. WK 2512 had the highest thousand-grain weight which was 86.63 g whereas WK 2546 had the lowest thousand-grain weight which was 12.189 g. e mean biological yield was 6.54 t ha −1 . WK 2388 had the highest biological yield having 10.01 t ha −1 whereas WK 2485 had the lowest biological yield having 3.65 t ha −1 . e mean harvest index was 0.237. WK 2540 had the highest harvest index with 0.404 whereas WK 2513 had the lowest harvest index with 0.036. e mean grain yield was 1.54 t ha −1 which is lower than the national average of 2.29 t ha −1 [3]. WK 2525 gave the highest yield, i.e., 2.50 t ha −1 , whereas WK 2513 gave the lowest yield with 0.27 t ha −1 .

Phenotypic and Genotypic Coefficient of Variation.
e highest values of GCV and PCV were obtained for the grain weight per spike (24.81% and 44.54%, respectively). e high difference between the PCV and GCV indicates that this trait is also highly influenced by environmental factors. ere was a very small difference between PCV and GCV values among the traits such as days to flag leaf appearance, days to booting, days to heading, days to anthesis, and days to maturity indicating that these traits are highly under genetic control. us, these traits are highly controlled by the genetic makeup of the genotype. High PCV (38.52%) and GCV (20.18%) values were estimated for the flag leaf area.
e difference between the PCV and GCV is pronounced which means that the flag leaf area is dependent on environmental factors. e difference between PCV and GCV for the trait plant height was 2.2 which means that the trait was not significantly affected by environmental factors. Similarly, the difference between PCV and GCV for the trait peduncle length was found to be 2.4 which means that the trait is mainly due to genetic factors and the environment does not play many roles in the expression of the trait.
Among the yield attributing traits, the difference between PCV and GCV was higher. is suggests that yield attributes are dependent on environmental factors and genetic factors are not sufficient for trait performance. e difference between the PCV and GCV is 14.4 for spike length, 19.7 for grain weight per spike, 10.9 for the number of grains per spike, 10.1 for 1000 grain weight, 7 for biological yield, 11.13 for grain yield, and 10.56 for harvest index. is suggests that yield and yield attributing characters are dependent on environmental factors as well.

Heritability and Genetic Advance as Percentage of
Mean. Higher broad sense heritability reveals that a large portion of variation is heritable to the offspring. e highest 4 International Journal of Agronomy  Table 3. is indicates that these traits are highly heritable to offspring. Similar reports were obtained by Khan and Naqvi [13]. e heritability of these traits is very high (>90%) which means that these traits are highly heritable. Plant height and peduncle length had higher heritability values (65.21% and 70.7%, respectively). Similar reports were obtained for the length of the peduncle by Yahaya [14]. Flag leaf area had low heritability (27.4%). is indicates that plant height and peduncle length were heritable to the offspring but the flag leaf area is not much heritable to the offspring. Among the yield attributing characters, the grain yield and harvest index (50.79% and 50.29%, respectively) had higher heritability. All yield attributing traits, i.e., grain weight per spike, number of grains per spike, 1000 grain weight, biological yield, and harvest index, had moderate heritability (30-60%). Similar results were obtained for 1000 grain weight and biological yield by Maragheh [15]. is suggests that yield attributing characters are moderately heritable to the off-springs. Spike length (13.98%) had low heritability.
For an efficient selection, the knowledge of heritability alone is not sufficient, and heritability estimates along with the genetic advance are considered more useful [16]. High heritability with high genetic advance as a percentage of mean was observed for days to flag leaf appearance (21.58%) while days to booting (19.78%), days to heading (18.29%), days to anthesis (17.39%), and days to maturity (10.75%) had high heritability with moderate genetic advance as a percentage of the mean. is indicates that these traits could be improved through phenotypic selection. High heritability with high genetic advance as a percentage of the mean was observed for peduncle length (22.40%) which indicates that the selection for this trait is effective. Similar results were obtained by [17]. High heritability with moderate genetic advance as a percentage of the mean (15.32%) was seen for the trait plant height. So, genetic improvement of these traits is effective through genetic selection. Khan and Naqvi [13] also obtained similar results under irrigated conditions. Among the yield attributing traits, moderate heritability with high genetic advance as a percentage of the mean was seen for grain weight per spike (28.48%), 1000 grain weight (23.1%), grain yield (41.02%), and harvest index (37.6%). Similar reports have been reported by Khan and Naqvi [13] for harvest index. Moderate heritability with moderate genetic advance as a percentage of the mean was seen for the number of grains per spike (19.2%) and biological yield (17.54%). Similar findings have been reported by Laghari et al. [17] for the number of grains per spike. Low heritability with low genetic advance as a percentage of the mean was seen for spike length (6.61%) [18,19], and also low to moderate heritability was found for spike length.

Correlation Coefficient Analysis.
Grain yield was positively and highly significantly correlated with grain weight per spike, the number of grains per spike, 1000 grain weight, biological yield, and harvest index shown in Table 4. Among them, the most yield correlated traits were harvest index followed by the number of grains per spike, grain weight per spike, biological yield, and 1000 grain weight. Donmez et al. [20] also found similar relation of grain yield with both harvest index and biomass. Similar results were reported for grain per spike and grain weight by Singh and Singh [21]. Grain yield had a highly significant negative correlation with days to flag leaf appearance, days to booting, days to heading, days to anthesis, days to maturity, and grain yield and was negatively and significantly correlated with plant height. is means that these traits negatively influence the grain yield.

Cluster Analysis.
All the 50 genotypes were clustered using 15 quantitative traits. e dendrogram as shown in Figure 2 revealed four clusters with a minimum of 31.86%  6 International Journal of Agronomy   International Journal of Agronomy 7 similarity level in UPGMA clustering. Wheat genotypes were classified into four cluster groups, namely, cluster 1, cluster 2, cluster 3, and cluster 4. e distance between the cluster's centroid was found the highest between clusters 1 and 4 and the lowest between clusters 2 and 4 presented in Table 5. Cluster 1 consisted of 43 genotypes which represent 86% of total genotypes. Genotypes grouped in this cluster showed the earliest appearance of flag leaf, booting, heading, anthesis, and maturity with the highest number of grains per spike, grain weight per spike, thousand-grain weight, biological yield, grain yield, and harvest index as shown in Table 6. Cluster 2 consisted of 5 genotypes including 10% of total genotypes. ese genotypes are characterized by the longest peduncle. e genotypes categorized into clusters 3 and 4 consisted of only 1 genotype in each. Cluster     International Journal of Agronomy characterized by the highest plants, the longest spike, and the smallest flag leaf area while cluster 4 took the longest period for flag leaf appearance, booting, heading, anthesis, and maturity with the largest flag leaf area, the shortest plant height and peduncle length, and also the lowest grain yield and harvest index.

Conclusion
Based on quantitative traits, WK 2495 was considered the best variety because of its earliness in flag leaf appearance, booting, heading, and anthesis. WK 2513 was the least desired genotype due to its late character and it is the lowest yield. Regarding the yield attributes, WK 2512 had the highest 1000 grain weight and grain weight per spike. WK 2525 gave the highest yield due to the difference in the genetic makeup of the genotypes. ough WK 2388 gave the highest biomass yield, its yield was significantly low because of very low grain weight per spike. From the study of genetic parameters, it was found that days to flag leaf appearance, days to booting, days to heading, days to anthesis, days to maturity, plant height, and peduncle length had high heritability with high to moderate genetic advance as a percentage of the mean indicating that these traits are effective for selection. Moderate heritability with high to moderate genetic advance as a percentage of the mean was observed for grain weight per spike, the number of grains per spike, 1000 grain weight, grain yield, biological yield, and harvest index indicating that these traits were prominent variables for selection for higher grain yield based on these traits. As revealed from correlation analysis, grain weight per spike, number of grains per spike, 1000 grain weight, biological yield, and harvest index were the most yield determinative traits. Hence, simultaneous selection of these traits might improve the grain yield of wheat.
Data Availability e authors can also make data available on request through a data access committee, institutional review board, or the authors themselves. In this case, they should name who should be contacted to request the data (e.g., the ethics or data access committee) and provide appropriate contact detail. Data will be provided by the corresponding author upon request.

Conflicts of Interest
e authors declare that they have no conflicts of interest.