Segnet Network Algorithm-Based Ultrasound Images in the Diagnosis of Gallbladder Stones Complicated with Gallbladder Carcinoma and the Relationship between P16 Expression with Gallbladder Carcinoma

The study focused on how to improve the diagnostic coincidence rate of patients with gallbladder stones and gallbladder cancer based on an optimized Segnet network algorithm and the relationship of gallbladder cancer with multiple tumor suppressor 1 (P16). 300 patients diagnosed with gallbladder cancer in the hospital were selected as the research subjects. The pyramid pooling operation was incorporated into the original Segnet network algorithm, and its performance was evaluated, factoring into the intersection of union (IoU), algorithm precision (Pre), and recall rate (Recall). After 8 hours of fasting, conventional ultrasound and contrast-enhanced ultrasound examinations were performed, and the images were evaluated by three experienced ultrasound diagnosticians. The positive signal of P16 immunohistochemical staining was brownish yellow, which was generally concentrated in the nucleus, and a small part was located in the cytoplasm. In each slice, ten visual fields were selected. Then, they were observed under a high-power mirror, and the number was counted. It was found that the optimized Segnet network algorithm increased the IoU by 7.3%, the precision by 8.2%, and the recall rate by 11.1%. The diagnostic coincidence rates of conventional ultrasound and contrast-enhanced ultrasound examinations for gallbladder cancer were 78.13% (25/32) and 87.5% (25/32), respectively. The positive expression rate of P16 in gallbladder adenocarcinoma (47.06%) was significantly lower than that of acute cholecystitis with gallbladder stones (84.38%) and gallbladder polyps (67.16%) (P < 0.05). The positive expression rate of P16 in patients with stage III and stage IV (33.33% and 40%) was significantly lower than that in patients with stages I and II (87.5% and 80%) (P < 0.05). The positive expression rate of P16 in high differentiation (86.67%) was significantly higher than that of moderate differentiation (40%) and poor differentiation (28.57%) (P < 0.05). In short, contrast-enhanced ultrasound can effectively improve the diagnostic coincidence rate of gallbladder cancer, and the expression of P16 in gallbladder cancer is closely related to tumor staging and differentiation.


Introduction
Gallbladder carcinoma, mainly adenocarcinoma, is a common malignant tumor in the extrahepatic biliary system [1]. In China, the average age of the onset of gallbladder cancer is approximately 56 years. Generally speaking, gallbladder cancer is more common in women. In addition, more than 85% of clinical gallbladder cancer patients have gallbladder stones [2]. e incidence of gallbladder cancer in patients with gallbladder stones is about 7 times higher than that of people without gallbladder stones. Among patients with gallbladder stones, the risk of gallbladder cancer in patients with a single gallbladder stone greater than 3 cm in diameter is 10 times that of patients with a gallbladder stone less than 1 cm in diameter. erefore, the cause of gallbladder cancer may be related to gallbladder stones [3]. e P16 gene, also known as multiple tumor suppressor 1 (MTS-1) gene, is a new type of tumor suppressor gene located on human chromosome 92P1. When the P16 protein is expressed at a low level, it will cause cell disorders and abnormal cell growth, and ultimately, it will lead to the formation of tumors [4]. For a long time, the main methods to diagnose gallbladder cancer included conventional ultrasound, computerized tomography (CT), endoscopic ultrasound, and magnetic resonance imaging (MRI) [5]. Conventional ultrasound can make accurate judgments on the morphological changes of gallbladder cancer, such as whether the size, location, and shape are regular, whether the bile duct is dilated, the degree of dilation, and the invasion of the adjacent tissue and lymph node metastasis, and it is recognized as the first choice to diagnose malignant tumors of the biliary system. Its diagnostic coincidence rate is approximately 80% [6]. Contrast-enhanced ultrasound can specifically diagnose tumors with rich and poor blood supply, and especially in the parenchymal stage, it can comprehensively scan the surrounding tissue of gallbladder cancer and detect lesions that conventional ultrasound cannot find [7]. Contrastenhanced ultrasound uses a contrast agent to perfuse the tissue. e peak time (TTP), peak intensity (PI), contrast agent perfusion rate, and other related parameters are recorded to determine the benign and malignant tumors. It significantly improves the diagnostic coincidence rate [8].
Deep learning can extract the essential features of the target by network training on a large amount to elevate the classification accuracy. During the training, it can not only automatically learn but also automatically modify the learning parameters [9]. In this research, a Segnet algorithm was proposed that can reduce the running time of the model while keeping the detection accuracy unchanged.
e Segnet algorithm can store the pooled index, avoid saving the feature map of the decoded part, and save memory [10]. e innovation of this study was to propose a new Segnet algorithm to process the conventional two-dimensional ultrasound image of 300 patients with gallbladder stones and gallbladder cancer. e immunohistochemical method was used to detect the expression level of the P16 protein in the cancer tissue. e primary objective of the study was to explore the accuracy, specificity, and sensitivity of contrast-enhanced ultrasound in the diagnosis of gallbladder cancer, as well as the relationship between the P16 protein expression and the tumor to fully understand the mechanism of gallbladder cancer and provide an evidencebased basis for the clinical diagnosis and treatment of gallbladder cancer.

Research Subjects.
In the study, 300 patients diagnosed with gallbladder cancer in the hospital from October 15, 2017, to April 25, 2021, were selected as the research subjects, including 135 males and 165 females, with an average age of (56.82 ± 12.74) years. Inclusion criteria include those (I) aged over 50, (II) suffering from gallbladder stones for more than five years, (III) with the diameter of stones greater than 2 cm, (IV) with the local gallbladder wall thickened, and (V) with gallbladder polyps greater than 1 cm. e clinical symptoms included dull pain, abdominal distension, indigestion, nausea, and vomiting. e experiment has been approved by the ethics committee of the hospital. e patients and their families understood the research and signed an informed consent form.

Segnet Network Algorithm.
e Segnet network algorithm mainly includes an encoding network and a decoding network. It can completely retain the feature information of the image, reduce the number of training parameters, shorten the training time, and display high-precision semantic segmentation images. e Segnet network algorithm comprises the convolutional layer, the normalization layer, the activation function, and the pooling layer ( Figure 1). e Segnet network algorithm structure has a symmetrical structure, with the left side of the network representing the encoding network and the right side representing the decoding network [11]. In the Segnet network, the pooling layer and the upsampling layer are used for image segmentation. e feature extraction of the target is completed by the left convolution layer, i.e., the encoding network. e pooling layer is used mainly to shrink the picture and perform deconvolution and upsampling operations [12]. Deconvolution is to make the classification features of the image more obvious. Upsampling is to restore the image to the same size as the input image. e encoding network extracts the features of the segmented image, transmits it to the decoding network, and outputs the semantic segmentation image ( Figure 2).
In the training process, the linear expression cannot fully meet the actual needs, and the Relu function is often used for fitting.
e Relu function is easy to calculate and fast to converge, and it is expressed as follows: When the input signal ≤0, the output is 0. When the input signal x > 0, the output is equal to the input.

Pyramid Pooling Operation.
In the original segnet network, the pooling operation will cause the loss of some high-frequency components in the image, produce a purification module, and lose the pixel position and spatial information ( Figure 3). To avoid this problem, the pyramid pooling operation is introduced. e pyramid pooling module uses different coarse and fine scales to fuse features, and the output of different scales includes feature maps of various sizes. It avoids producing fuzzy blocks as much as possible and retains the original features extracted by the convolutional neural network (CNN) (Figure 4).

Evaluation Indicators of Experimental Results.
ere are three evaluation indicators to determine the accuracy of feature extraction. e first is the intersection of union (IoU). It represents the degree of overlap between the candidate area generated when detecting the target and the original marked area, and it is expressed as follows: where T1 represents a correctly detected nontarget feature, S1 represents a nontarget feature that is erroneously detected as a target feature, and S2 represents a target feature that is erroneously detected as a nontarget feature. e second is the precision (Pre), which is the percentage of real target pixels in the detected target features, and it is expressed as follows: where T1 represents a correctly detected nontarget feature, and S2 represents a target feature that is incorrectly detected as a nontarget feature. e third is the recall rate (Recall). It is the ratio of the detected real target pixels to all the ultrasound samples of the test set and is expressed as follows: where T1 represents the nontarget feature that is correctly detected, and T2 represents the target feature that is correctly detected.    the location, number, size, shape, boundary, and thickness of the gallbladder wall were recorded. Color ultrasound diagnostic apparatus was used for contrast-enhanced ultrasound, and an abdominal probe with a frequency of 3.5 MHz was used to detect the blood flow signal and shape of the lesion and determine the blood flow velocity. After angiography, three experienced ultrasound diagnosticians evaluated the images independently.

Immunohistochemical Staining Procedure and Result
Judgment. e P16-positive gastric cancer tissue was used as a positive control, and the phosphate buffered saline (PBS) instead of P16 primary antibody was used as a negative control. e specific steps were as follows: Firstly, after being dewaxed and hydrated, the paraffin sections were rinsed with PBS (PH � 7.4) three times for 3 minutes each time. en, according to the requirements of each antibody, the tissue antigen was repaired accordingly. Each slice was immersed in 50uL peroxidase blocking solution to incubate for 10 minutes at room temperature to block the activity of endogenous peroxidase. en, it was rinsed with PBS three times for 3 minutes each time. Next, each slice was immersed in 50uL normal nonimmune animal serum to incubate for 10 minutes at room temperature. Subsequently, the serum was removed, and each slice was immersed in 50uL of the primary antibody to incubate for 60 minutes at room temperature. Again, it was rinsed with PBS three times for 3 to 5 minutes each time. After the PBS solution was removed, each slice was immersed in 50uL biotin-labeled secondary antibody to incubate for 10 minutes at room temperature. en, it was rinsed with PBS three times for 3 minutes each time. After the PBS solution was removed, each slice was immersed in 50uL streptavidin-peroxidase solution to incubate for 10 minutes at room temperature. en, it was rinsed with PBS three times for 3 minutes each time. After the PBS solution was removed, each slice was immersed in 100uL of freshly prepared diaminobenzidine (DAB) solution and observed under a microscope for 3 to 10 minutes. Next, it was rinsed with tap water and stained with hematoxylin, followed by rinsing with PBS or tap water to return to blue. DAB was used for color development. e slices were dehydrated and dried with gradient alcohol, cleared with xylene, and mounted with neutral gum. e positive signal of P16 immunohistochemical staining was brownish-yellow, generally concentrated in the nucleus, with a small part in the cytoplasm. In each slice, 10 fields of view were selected, which were then observed under a high-power mirror to count the number. More than 60% of the cells in the field of view with brown-yellow particles was defined as strong positive (+++), 30% to 60% of the cells with brown-yellow particles was defined as positive (++), 5% to 30% of cells with brown-yellow particles was defined as weakly positive (+), and no obvious brown particles in the cell were defined as negative (-). A weak positive expression or above was considered a positive expression.

Statistical Methods.
In this study, SPSS21.0 statistical software was used for statistical analysis of the result data. e calculated data that conformed to the normal distribution were represented by the mean standard deviation (x ± s), and the calculated data that did not conform were represented by the percentage (%). e comparison of counting data adopts χ 2 test. P < 0.05 indicated that the difference was significant.

Performance of the Segnet Network Algorithm.
e Segnet and the optimized Segnet with the pyramid model were trained separately. Figure 5 shows the loss value/accuracy change curve of the training set and the validation set. Figure 6 shows the semantic segmentation network. According to the result data, the optimized Segnet network algorithm of the pyramid pooling operation increased the IoU by 7.3%, the precise (Pre) by 8.2%, and the recall rate (Recall) by 11.1%. It suggested that the semantic segmentation model with pyramid pooling elevated the accuracy and speed of target extraction.

Results of Conventional Ultrasound
Examination. 25 cases of gallbladder cancer were detected by conventional ultrasound examination. e diagnosis coincidence rate was 78.13% (25/32). 4 cases of nodular gallbladder carcinoma had nodular protrusions in the gallbladder cavity, wide basement, irregular edges, and heterogeneous internal echoes. e abnormal central echoes caused by stones, air, or necrosis were noted (Table 1). In 5 cases of thick-walled gallbladder carcinoma, the gallbladder wall was locally thickened or diffusely unevenly thickened, showing hyperechoic (more common) or hypoechoic signals, and the entire gallbladder was stiff, deformed, and the wall was rough or irregular. In 5 cases of solid gallbladder carcinoma, the entire gallbladder showed disordered low-echo or medium-echo solid masses, and the dark areas in the gallbladder cavity disappeared, often accompanied by gallbladder stones. ere were 6 cases of mixed gallbladder carcinoma, and both thick-walled and nodular types were noted (Figure 8).

Results of Contrast-Enhanced Ultrasound Examination.
28 cases of gallbladder cancer were examined by contrastenhanced ultrasound, and the diagnosis coincidence rate was 87.5% (25/32). For patients with gallbladder stones combined with gallbladder cancer, the mass was large, the boundary was not clear, and the surrounding halo was visible.
ere were irregular liquid dark areas inside, the mass was adjacent to the gallbladder, and the boundary with the gallbladder was not clear (Figure 9; Table 2).

Immunohistochemical Staining Results of P16.
e positive expression of P16 was mainly concentrated in the nucleus and cytoplasm, with brownish-yellow particles, and some cell membranes were also stained ( Figure 10).

Expression of P16 in Lesions of Gallbladder Carcinoma.
In 17 cases of gallbladder adenocarcinoma, the positive expression rate of P16 was 47.06%. In 67 cases of gallbladder polyps, the positive expression rate of P16 was 67.16%. In 32 cases of acute cholecystitis with gallbladder stones, the positive expression rate of P16 was 84.38%. In 36 cases of chronic cholecystitis with gallbladder stones, the positive expression rate of P16 was 81.56%. e positive expression rate of P16 in gallbladder adenocarcinoma was significantly lower than that in patients with other gallbladder lesions, and the difference was significant (P < 0.05) (Figure 11).

e Relationship between the Positive Expression of P16
and Pathological Features. As per the clinical staging of gallbladder cancer, there are 8 cases in stage I, 5 cases in stage II, 9 cases in stage III, and 10 cases in stage IV. e positive expression rate of P16 in the patients of stages III and IV (33.33% and 40%) was significantly lower than that of the patients of stages I and II (87.5% and 80%), and the difference is significant (P < 0.05) (Figure 12).
According to the results of tumor differentiation, 7 cases were poorly differentiated, 10 cases were moderately differentiated, and 15 cases were highly differentiated. e positive expression rate of P16 in the high differentiation (86.67%) was significantly higher than that of moderate Journal of Healthcare Engineering differentiation (40%) and poor differentiation (28.57%), and the difference was significant (P < 0.05) (Figure 13).

Discussion
Gallbladder cancer is a common malignant tumor of the biliary system, and its incidence has been increasing in recent years. Gallbladder cancer combined with gallbladder stones continuously stimulates the gallbladder wall mucosa, causing the abnormal proliferation of mucosal epithelial cells and cholestasis. Gallbladder cancer lacks specific clinical manifestations in the early stage and has similar symptoms of gallbladder stones. erefore, when it is discovered, it is already in the middle and late stages when the best treatment opportunity has been lost [13,14]. At present, the early diagnosis of gallbladder cancer combined with gallbladder stones is a clinical problem that needs to be solved urgently. Conventional ultrasound examination is easy to operate, noninvasive, low in price, and easy to be accepted by patients. It is recognized as the first choice for the diagnosis of gallbladder cancer [15]. However, because of the limitations     of the technology itself, the microvessels of the lesion cannot be well-evaluated. In this study, 25 cases of gallbladder cancer were detected by conventional ultrasound, and the diagnosis coincidence rate was 78.13% (25/32), which was consistent with the research results of Rana et al. (2016) [16]. ere were a total of 7 cases of missed and misdiagnosed cases. e main reasons were as follows: firstly, there is a certain amount of bile mud and viscous bile deposited in the gallbladder, and they do not move when the body position changes. Secondly, the thickness of the normal gallbladder wall is approximately 2 mm. When it is greater than 4 mm, the stone has an arc shape, and it is easy to misdiagnose the gallbladder with a thickened gallbladder wall and gallbladder cancer as chronic cholecystitis and gallstone disease. irdly, there are many organs around the gallbladder and abundant vessels. After gallbladder cancer has infiltrated, the outline of the gallbladder is unclear, and it is easy to be misdiagnosed as a tumor in the surrounding tissue.
Contrast-enhanced ultrasound technology is an imaging technology to understand the anatomy of tumor blood vessels. It can enhance the backscattered echo of the cells and improve the diagnostic coincidence rate. However, the lowresolution results in the unclear intima of large blood vessels, and it is unable to identify tiny blood vessels [17]. In this study, 28 cases of gallbladder cancer were detected by contrast-enhanced ultrasound, and the diagnosis coincidence rate was 87.5% (25/32). Contrast-enhanced ultrasound can observe the blood flow characteristics of the lesions of gallbladder cancer patients in real time, accurately display the spatial distribution of vascular perfusion, and significantly improve the signal ratio of detection. In this study, it can accurately detect the invasive growth of gallbladder tumors, providing a theoretical basis for the diagnosis of gallbladder cancer. e P16 gene is a tumor suppressor gene directly involved in the negative feedback regulation of the cell cycle, and its level is closely related to the cell cycle [18,19]. When the P16 gene has mutations or deletions, it will lead to a low expression or the inactivation of the P16 protein, leading to cell cycle disorders [20]. In this study, the positive expression rate of P16 in gallbladder adenocarcinoma (47.06%) was significantly lower than that of acute cholecystitis with gallbladder stones (84.38%) and gallbladder polyps (67.16%) (P < 0.05). It showed that P16 was obviously missing in gallbladder cancer tissue, and the deletion and mutation of the P16 gene would lead to the occurrence of gallbladder cancer. e positive expression rate of P16 in the high differentiation (86.67%) was significantly higher than that in the middle differentiation (40%) and poor differentiation (28.57%) (P < 0.05), indicating that the positive expression of P16 protein in the gallbladder cancer tissue was related to the degree of tissue differentiation.

Conclusion
In this study, the ultrasound images based on the optimized Segnet network algorithm were used to diagnose 300 patients with gallbladder stones and gallbladder cancer. It was found that contrast-enhanced ultrasound can effectively improve the diagnostic coincidence rate of gallbladder cancer, and the expression of P16 in gallbladder cancer was closely related to tumor staging and differentiation. However, some limitations in the study should be noted. e sample size is small, which will reduce the power of the study. In the follow-up, an expanded sample size is necessary to strengthen the findings of the study. In conclusion,  Figure 13: e relationship between the positive expression of P16 and the degree of differentiation. * indicates that compared with high differentiation, the difference was significant, i.e., P < 0.05.  Figure 11: Positive expression of P16 in the lesions of gallbladder cancer. * indicates that compared with gallbladder adenocarcinoma, the difference was significant, i.e., P < 0.05.

Data Availability
e data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest
e authors declare no conflicts of interest.