Phytochemical Study on Antioxidant and Antiproliferative Activities of Moroccan Caralluma europaea Extract and Its Bioactive Compound Classes

Background Caralluma europaea (C. europaea) is a medicinal plant used in Moroccan popular medicine. Objective of the Study. The present work was aimed at identifying the chemical composition and the antioxidant and antiproliferative activities of hydroethanolic and bioactive compound classes of C. europaea) is a medicinal plant used in Moroccan popular medicine. Materials and Methods. The chemical composition was analyzed using HPLC. The antioxidant power was determined using both DPPH and FRAP assays. The antiproliferative activity was effectuated against cancerous cells using WST-1. Results The chemical analysis showed the presence of bioactive constituents such as quercetin, myricetin, and hesperetin. The polyphenol and flavonoid contents were estimated at 51.42 mg GA/g and 20.06 mg EQ/g, respectively. The EC50 values of FRAP assay of hydroethanolic, flavonoid, saponin, and mucilage extracts were 5.196 mg/ml, 4.537 mg/ml, 3.05 mg/ml, and 6.02 mg/ml, respectively. The obtained IC50 values with the DPPH test were 1.628 mg/ml, 1.05 mg/ml, 1.94 mg/ml, and 9.674 mg/ml, respectively. Regarding MDA-MB-231, saponins were highly effective even with the lowest concentration (15.62 μg/ml). The flavonoids decreased the cell viability with IC50 values of 43.62 ± 0.06 μg/ml). The flavonoids decreased the cell viability with IC50 values of 43.62 ± 0.06 μg/ml). The flavonoids decreased the cell viability with IC50 values of 43.62 ± 0.06  Conclusion The present results suggest that C. europaea) is a medicinal plant used in Moroccan popular medicine.


Introduction
Traditional medicine is attracting more interest and its use has increased over the past three decades. According to the WHO, more than 80% of African people still rely on popular medicine for their benefit care including cancer treatment [1]. Nowadays, many pharmaceutical agents have been discovered in medicinal plants. However, the potential of plants as sources for the production of new drugs is largely untapped [2]. Medicinal plants play an important role in folk medicine, and they have been described in worldwide pharmacopoeia [3].
Caralluma europaea is a wild species frequently used in Moroccan traditional medicine for its presumed anticancer activity [4]. C. europaea belongs to the Apocynaceae family, and it is widely spread in Southern Jordan and the North African coast of the Mediterranean Sea [5].
Nowadays, the world witnesses a great revolution in cancer treatment due to the large development of novel pathways of treatment. Although that resistance to classical agents continues to be a major problem in cancer therapies [10]. It was earlier reported that the medicinal plants play an alternative role in breast cancer treatment [11,12].
e objective of the present study was to screen the phytochemical composition and the antioxidant and antiproliferative effects of both aerial parts hydroethanolic and natural bioactive classes of C. europaea using the methods described in the next section.

Plant Material. Caralluma europaea was collected in
February 2016, from the Imouzzer region at the Middle Atlas Mountains, Morocco, using GPS coordinates (33°44′ North, 5°01′ West). e plant was authenticated by Professor Bari Amina, and a voucher specimen, 18I4C001, has been deposited in the herbarium of the Department of Biology, Faculty of Sciences Dhar El Mahraz, Fez University.

Preparation of Hydroethanolic Extract.
e aerial parts of the plants were washed with water. After drying in the shade, the material was ground into a fine powder using a grinding apparatus. 10 g of the powder was extracted with 100 ml of hydroethanolic solution (7 : 3 v/v ethanol: distilled water) for 45 min. e mixture was filtered, then concentrated at low temperature, and finally preserved at −80°C until use.

Determination of Total Antioxidant Compounds
e polyphenolic content (TPC) was determined using Folin-Ciocalteu reagent as previously described in the literature. e results were expressed as mg gallic acid equivalent (mg GAE)/g dry extract [17].

Total Flavonoid Content.
e flavonoid content (TFC) was determined according to the method in earlier data. e results were expressed as mg quercetin equivalent (mg QE)/g dry extract [18].

HPLC Analysis.
Polyphenolic extract of Caralluma europaea was analyzed using high-performance liquid chromatography (Agilent Technologies) equipped with a quaternary pump. e sample was detected by using a UV detector operating at 280 nm. A volume of 10 ml of the extract was injected over a C18 ZORBAX Eclipse Plus (serial number: USUXBO2265) (46 * 150 nm; 5 μm) column, at a flow rate of 1 ml/min, and the column temperature was maintained at 25°C. e mobile phase was composed of acidified water 0.1% (A) and acetonitrile (B) with a total running time of 65 min. e test of DPPH was performed using the methods reported in [19], with slight modifications. A solution of the DPPH was prepared by dissolving 100 ml of methanol and 2.4 mg DPPH. 980 μl of DPPH solution (60 μM) was mixed with 20 μl of an extract with concentrations ranging from 10 to 50 μg ml −1 . After 2 h of incubation, the absorbance was determined at 517 nm. BHT (10 to 50 μg ml −1 ) was used as a positive control. e percentage of inhibition of DPPH was calculated using the formula given as follows: where IP is the inhibition percentage, A0 is the absorbance of the DPPH solution without extract, and A is the absorbance of the DPPH solution with the extract.

Ferric Reducing Antioxidant Power (FRAP)
. e FRAP method determines the capacity of antioxidants to reduce Fe +3 to Fe +2 . 1 ml of extract was dissolved with 2.5 ml of phosphate-buffered saline and 2.5 ml of potassium ferricyanide (1%). After incubation for 20 min at 50°C, 2.5 ml of trichloroacetic acid (10%) was added. e obtained solution was centrifuged for 10 min at 3000 rpm. en, 2.5 ml of the supernatant was combined with 0.5 ml of FeCl 3 (0.1%) and 2.5 ml of distilled water. e absorbance was determined spectrophotometrically 2 Evidence-Based Complementary and Alternative Medicine at 700 nm. Ascorbic acid was considered as a reference [20].

Extraction of Some Bioactive Compound Classes from
Caralluma europaea 2.7.1. Flavonoids. e powder of C. europaea (30 g) was macerated with 100 ml of methanol (MeOH) for 72 h at room temperature. e mixture was filtered and concentrated at 60°C. e obtained residue was dissolved in distilled water and then extracted again successively three times with 3 × 30 ml (chloroform, diethyl ether, n-hexane, ethyl acetate, and nbutanol) to get respective extracts. e flavonoids contained in the last phase were recovered and then subjected to evaporation at 55°C in order to obtain the dry residue [21].

2.7.2.
Saponins. 10 g of the powder of C. Europaea was initially defatted with petroleum ether for 2 h. en, the extraction was carried out with 300 ml of ethanol for 24 h. e obtained extract was fractionated with petroleum ether and distilled water in equal proportions. 150 ml of the aqueous portion was added to n-butanol. e n-butanol fraction was separated, the remained solution was concentrated, and then the saponins were dried in an oven [22].

2.7.3.
Mucilages. 10 g of the powder of C. Europaea was macerated with distilled water for 5 or 6 h, boiled for 30 min, and kept aside for 1 h. e material was filtered through a muslin cloth. Acetone was added to the filtrate in order to precipitate the mucilage. e mucilage was separated and dried at a temperature under 50°C. e dried mucilage was powdered and stored for further use [23].

Evaluation of Antiproliferative Activity
e antiproliferative activity of natural compounds from the aerial parts of C. europaea was tested against two human breast cancer cell lines, MDA-MB-231 and MCF-7, provided by Dr. L'Houcine Ouafik (Laboratoire de Transfert d'Oncologie, Marseille). e cells grew in DMEM medium supplemented with glutamine (1%), fetal calf serum (10%), and a mixture of streptomycin/penicillin (1%). Cells were preserved at 37°C.
At the end of the treatment period, the medium was removed, and 10 μl of WST-1 reagent was added to each well. e plates were incubated again (4 h at 37°C). Cell viability was performed by absorbance reading of each well at 450 nm using a Wallac Victor X3 multiplate reader. Untreated cells were considered as a negative control. Mitomycin was considered a positive control. e IC 50 value responsible for 50% of cell growth inhibition was determined by plotting the inhibition percentage versus the concentrations (μg/ml).

Statistical Analysis.
Analysis of the dose-response curves and the IC 50 values was done using GraphPad Prism 5. Calculation of confidence limits and significance testing were made at the level of p � 0.05 (p value <0.05 was considered significant).

Qualitative Phytochemical Screening.
e results of the phytochemical analysis of hydroethanolic extracts of C. europaea revealed the presence of flavonoids, catechic tannins, triterpene saponins, mucilages, coumarins, oses, and holosides and the absence of quinones and alkaloids (Table 1).

Total Polyphenol and Flavonoids Content.
e phenolic content of aerial parts of C. europaea was determined from the calibration curve (y � 1.575x + 0.022; R 2 � 0.959). e value was 51.42 ± 0.003 mg gallic acid equivalent/g of extract.
Regarding the total flavonoid content (y � 0.814x + 0.021; R 2 � 0.953), the value was 20.06 ± 0.007 mg QE/g of extract ( Figure 1). e obtained results in this study showed a significant level of phenolic compounds contained in aerial parts of C. europaea. According to HPLC analysis, the rate of polyphenols contained in Caralluma europaea extract ranged from 0.034 to 2.772 μg/ml. e ferulic acid concentration was determined at 2.772 μg/ml, followed by quercetin with 0.77 μg/ml. e concentrations of myricetin, gallic acid, and hesperetin were estimated at 0.350 μg/ml, 0.135 μg/ml, and 0.034 μg/ml, respectively ( Table 2).

Evaluation of Antioxidant Activity Using FRAP and DPPH Assay.
e antioxidant activity of C. europaea extract was determined using two methods: DPPH and FRAP tests.
e results vary according to the used methods.

Evaluation of Antioxidant Activity Using FRAP Assay.
As indicated in Figure 3, all extracts showed lower antioxidant activities than the standard (ascorbic acid) (Figure 4). e EC 50 values of hydroethanolic, flavonoids, saponins, and mucilages Evidence-Based Complementary and Alternative Medicine 3 fractions were 5.196 mg/ml, 4.537 mg/ml, 3.05 mg/ml, and 6.02 mg/ml, respectively.

Evaluation of Antioxidant Activity Using DPPH Assay.
As shown in Figure 5, the percentage of free radical inhibition of hydroethanolic extracts such as flavonoids, saponins, and mucilages was lower than that of ascorbic acid ( Figure 6). e antioxidant activity of the flavonoids fraction (IC 50 � 1.51 mg/ml) was more pronounced compared to the other hydroethanolic extracts (IC 50 � 1.628 mg/ml), saponins (IC 50 � 1.94 mg/ml), and mucilages (IC 50 � 9.674 mg/ ml). e statistical analysis showed a significant difference between the IC 50 values of all tested extracts and that of the ascorbic acid (IC 50 � 0.23 mg/ml) (p value <0.05).

Extraction of Natural Antioxidants from C. europaea.
Genus of Caralluma is characterized by the presence of bioactive components such as glycosides and steroids, known for their various biological activities [25][26][27]. In order to investigate the potential antiproliferative effect of C. europaea natural compounds, we proceeded with the extraction of saponins, flavonoids, and mucilage. e hydroethanolic extract was also tested for its antiproliferative effect. e extraction yields of various fractions were reported in Table 3.

Antiproliferative
Activity. e antiproliferative effect of hydroethanolic extract of C. europaea and its fractions against MDA-MB-231 cell lines was evaluated using the WST-1 assay. Figure 7 shows the effect of hydroethanolic extract and its fractions at different concentrations

Discussion
Cancer is one of the most lethal diseases worldwide, requiring effective prevention and treatment measures. erefore, considerable research has been conducted to develop novel natural chemotherapeutic agents. In medicine, plant-derived compounds can be used as traditional, modern drugs, or chemical units for synthetic drugs. Antiproliferative activity models provide data to select extracts of plants with antitumoral potential for future studies [28].
Genus Caralluma arouses great interest in scientific fields due to its attractive immunostimulatory and pharmacological activities [29]. ese activities could be due to its phytochemicals including glycoside and pregnane steroid [26,27]. In Morocco, Caralluma europaea is widely used in folk medicine for its presumed anticancer activity [30]. However, no scientific data about its potential anticancer activity against cancerous cell lines were reported to the best of our knowledge. e hydroethanolic extract of C. europaea was investigated for its phytochemical compounds, the total phenolic and flavonoid contents, and the antioxidant and antiproliferative activities. e qualitative phytochemical study showed the presence of different classes of bioactive secondary metabolites, such as flavonoids, triterpene saponins, oses, and holosides, previously described to have various medicinal activities [31]. Regarding the studied activities, our results were in accordance with those reported for C. sinaica [32].
It is worth noting that C. europaea is rich in phenolic compounds; the total phenolic content of C. europaea hydroethanolic extract was 51.42 ± 0.003 mg GAE/g dry of extract. ese values were significantly higher than those of the methanolic and aqueous extract of C. adscendens, being 21.0 ± 0.59 and 18.8 ± 0.98 mg GAE/g dry extract, respectively [33].
Phenolic compounds are ubiquitous plant metabolites and the major group of compounds that contribute to the antioxidant properties; they can play an important role in the initiation of deleterious free radical actions [34]. Species of the genus Caralluma have been known for their richness in phenolic compounds [35,36].  Our study also demonstrated that the hydroethanolic extract of C. europaea was rich in flavonoids compounds. e total flavonoids in the hydroethanolic extract of C. europaea were 20 ± 0.007 mg QE/g dry.
is content is still relatively higher compared to methanolic and aqueous extracts of C. adscendens with values of 3.7 ± 1.16 and 3.1 ± 0.54 mg QE/g dry weight, respectively [33].
Flavonoids are the most important natural phenolic compounds and are thought to be responsible for the antioxidant and the antiproliferative activities of natural products [37,38]. Many species of Caralluma have been known for the presence of interesting amounts of bioactive compounds [39,40].
Flavonoids and polyphenols are natural antioxidants, known to be very active scavengers of many free radicals [41]. DPPH test is a method for estimating free radicalscavenging activity of antioxidants. e FRAP test determines the ability of these complexes to break the free radical chain by donating an atom of hydrogen [42,43]. In the current study, the antioxidant activity of C. europaea hydroethanolic extract was determined using DPPH and FRAP assays. Using the DPPH method, all tested extracts of C. europaea showed a lower antioxidant power compared to ascorbic acid. e IC 50 values of hydroethanolic, flavonoids, saponins, and mucilages extracts were 1.628 mg/ml, 1.51 mg/ml, 1.94 mg/ml, and 9.674 mg/ml, respectively, which were significantly lower than that of the ascorbic acid (0.23 mg/ml). Furthermore, the obtained IC 50 values with FRAP test concerning hydroethanolic, flavonoids, saponins, and mucilages fractions were 5.196 mg/ml, 4.537 mg/ml, 3.05 mg/ml, and 6.02 mg/ml, respectively. ese results can        Potential antiproliferative and anticancer activities of some species of Caralluma were reported in many earlier research works.
Antiproliferative activity of plants may be attributed to the presence of alkaloids, glycosides, saponins, and polyacetylenes [46]. A previous phytochemical study on Caralluma species suggested the presence of glycosides and saponins [47]. Saponins are natural compounds that have several pharmacological activities like antiproliferative properties [48,49], with pronounced effects on malignant tumor cells [9].
Numerous in vitro studies have reported the antiproliferative activity of saponins on breast cancer, cervical colon, and hepatic carcinomas. It was reported that several signaling pathways have been identified to be responsible for cell death [49,50]. e antiproliferative effect of saponins resulted in the activation of different signaling pathways [49], whereas the induction of apoptosis is the most involved pathway [51]. Saponins have an important effect on some experimental tumors, such as estrogen receptor-negative mammary carcinogenesis [52].
In the present research work, flavonoids showed a significant antiproliferative effect with IC 50 values of 43.62 ± 0.06 μg/ml. is class of molecules possesses a large range of pharmacological activities including antiproliferative activity. e flavonoids constitute the most important group of polyphenolic compounds, which have been investigated for their antiangiogenic, antiproliferative, and antiestrogenic effects [53]. Several works have reported that the flavonoids possess antiestrogenic activity and prevent the development of hormone-dependent cancers [54].

Conclusion
e findings of the present work revealed that the extract of aerial parts and the natural bioactive classes of C. europaea exhibit promoting antioxidant and antiproliferative activities.
erefore, C. europaea may constitute an important source of bioactive compounds which may lead to conceptualizing new effective drugs against cancer as well as diseases due to free radicals.

Data Availability
e data used to support the findings of this work are included within the article.