Floristic Diversity and Natural Regeneration Status of Entoto Mountain and the Surrounding Area in Addis Ababa, Ethiopia

The study was carried out at Entoto Mountain and its surrounding area, about 10 km north of the center of Addis Ababa. The purpose of this study was to assess the floristic composition and regeneration status of woody species and recommend further conservation methods. Ten transect lines were laid from south-north at 1 km interval. Along these transect lines, 62 sample plots of 400 m2 (20 m × 20 m) were laid at 1 km interval. A total of 179 plant species belonging to 107 genera and 60 families were recorded. Asteraceae (30 species) was the most dominant family. Of 179 plant species, 73 were naturally regenerated woody species representing 48 genera and 34 families. Herbs account for the largest growth form (91, 50.84%), indicating the fact that disturbance favors herb species. For the analysis of vegetation diversity, woody species composition, and density, the study area was classified into five land-use types. Of the five land-use types, degraded land-use type had low species diversity and evenness (1.48 and 0.295), and it had a low density of economically and ecologically important larger trees. However, the density of seedlings and saplings showed the normal regeneration status for the herbs and shrubs. Therefore, responsible stakeholders should give high priority for the conservation of ecologically and economically important large trees using appropriate conservation methods in the study area.


Introduction
Ethiopia is an important regional center of biological diversity, and the flora and fauna have a rich endemic element [1,2]. e country has the fifth largest flora in tropical Africa. Vegetation types in Ethiopia are highly diverse, varying from Afroalpine to desert vegetation. However, the vegetation resources of the country have been reduced due to various factors. e most prominent ones are deforestation, expansion of agricultural land, overgrazing, unsustainable utilization, invasion of exotic species, and overexploitation for various purposes such as firewood, charcoal, construction material, and timber, all spurred by rapid human population growth [3,4].
Plantations in general and Eucalyptus plantation in particular can have a catalytic effect on the regeneration of native species and can be used as a management tool for restoration of degraded forest lands [5,6]. Because of the considerable damage of the mountain forests, concern has arisen about the natural regeneration of indigenous species. Regeneration is thus defined as the reassembly of floristic and structural diversity back to self-perpetuating climax states [7]. In Ethiopia, the uncontrolled removal of trees and land disturbance, such as collection of firewood, cultivation of lands, and grazing, severely reduce the density of the species and affect regeneration [8].
In the Ethiopian highlands, which suffer from severe deforestation and biomass fuel crises, Eucalyptus is the prominent tree in government and community estate plantations because of its ready propagation through coppicing, resistance to browsing by livestock, and rapid growth rate. Currently, about 55 Eucalyptus species are available in Ethiopia [9]. However, the most common and widespread Eucalyptus species include Eucalyptus globulus Labill. e natural regeneration of plants is an important subject in both conservation biology and management [10]. Cost-effective plant regeneration, especially natural regeneration (i.e., regeneration of native plant species), is the keystone of sustainable forestry [11,12]. Natural regeneration depends on the seed bank [13]. In this study, our objective was to identify the vegetation resources and the current regeneration status of woody species of the Entoto mountain range that lies on the Northwestern Entoto, Central Yeka, Ankorcha mount, and Northeastern Yeka Abado through Gurara within the limit of the city of Addis Ababa, Ethiopia, where the integrated tourist destination development programs would take place.

Description of the Study Area.
e study area belongs to dry evergreen Afromontane forest and grassland complex (DAF) in the central highlands of Ethiopia [14]. Mount Entoto is considered to be the highest peak overlooking the city of Addis Ababa. Its altitude reaches 3,200 meters above sea level and is part of the Entoto mountain chain. is study area is located in two subcities of Addis Ababa, namely, Gullele and Yeka subcities.
e study was conducted in Entoto mountain and its surrounding area that surrounds the city Addis Ababa between latitudes 9°08′N-10°06′N and longitudes 37°47′E-37°48′E. e altitude range of the study area is between 2551 m and 3031 m a.s.l.
is study was undertaken in the Entoto mountain range that lies on the southeastern slopes of Mt Entoto, between the northern limit of the city of Addis Ababa, and the track along the mountain ridge. As can be seen from the underneath map, the elevation of the area ranges from 2,440 m to 3,196 m a.s.l. (Figure 1).

Climate of the Study Area.
From the 10-year (2007 to 2016) temperature and rainfall data collected from [15], the mean annual rainfall of the area is about 1226 ml. As far as the monthly rainfall record of the area is concerned, the area mostly receives its maximum rainfall between June and September. is is normally considered as the rainy season (kiremt) in the area. Low amount of rainfall is recorded between October and April. e average annual temperature of the area is 14°C . e maximum and minimum average annual temperatures of the area for the last ten years are 17.89 and 8.85°C, respectively. roughout this period, the relatively maximum temperature was recorded during the months of January (18.4°C), February (19.7°C), March (20.12°C), April (19°C), and May (19.15°C), which is above the mean annual temperature (17.81°C) of the stated period. e lowest temperature was recorded during the month of August (14.49°C).

Methods of Data Collection
2.3.1. Sampling Design. In this study, a systematic sampling design was used to collect data on vegetation and topographic variables. Ten transect lines consisting of 62 plots of size 20 m × 20 m (400 m 2 ) were systematically laid in south-north directions using compass. Five plots each was laid in eight transect lines and six plots each in the last two transect lines. e distance between consecutive plots along the transect lines was from 500 m to 1 km. Five transects were laid at 2 km distance and three transect lines at 1.5 km and the last two at 3 km distance from each other. Trees and shrubs were collected from the larger size plots whereas the nested small subplots (five 1 m * 1 m plots; four in the corner and one in the center of the larger plot) were used for seedling and sapling and herbaceous data collection.
For the sake of data collection and analysis, all the plots were categorized into five land uses through visual observation. ese include Eucalyptus globulus plantation forest (EPF), plantation forest (PAF), natural forest (NAF), degraded land (DEL), and riverine vegetation (RV).

Data Collection.
In each quadrat, all trees, shrubs, climber, and herbs were recorded. Plant species occurring outside the quadrat but inside the study area were also documented. In addition, all of the naturally regenerated woody species were identified and counted from each plot. Individuals were categorized into three size groups as follows [16]: seedling (height ≤1.0 m), sapling (height between 1 and 3 m), and tree/shrub (height >3 m). During the study, physiographic variables such as altitude, longitude, and latitude were measured for each plot using GPS. Taxonomic identification was made following the Flora of Ethiopia and Eritrea, Honey bee Flora of Ethiopia, and consultation with experienced taxonomic experts.

Data Analysis.
Plant species recorded in all plots were used in the analysis of the vegetation data. For analysis of vegetation data, Shannon and Wiener index of species diversity [17], species evenness, and Jaccard's coefficient of similarity were used. Shannon's index takes into account the evenness of abundance of species. e ratio of observed Shannon index to maximum diversity (H max � ln S) can be taken as a measure of evenness (E) [18][19][20]. Similarities of vegetation of the five land-use types were also compared using Jaccard's coefficient of similarity (JCS) [19]. Density of the selected plant species was compared among the five landuse types. e frequency and relative frequency of the selected dominant plant species were presented for the study area. Similarly, the growth form of all the identified species in five land-use types was presented in the diagram. All the analyzed outputs of the vegetation data were presented in the form of table and diagram to indicate the areas in terms of ecological and economic significance.

Floristic Composition and Diversity of the Study Area.
A total of 179 plant species belonging to 107 genera and 60 families were identified from the study area (see Table 1). e total numbers of individual species in their respective growth form against different land-use types are indicated in Figure 2. Herbs were dominant and represented by (91) 50.84% species, shrub by (46) 25.67%, tree by (26)14.53%, and tree/shrub by (9) 5.03% species while climber by (7) 3.9% species. From the total woody species, Eucalyptus globulus (952/ha), Juniperus procera (369/ha), and Carissa spinarum (304/ha) were the most abundant species in the study area, whereas species such as Millettia ferruginea, Ficus sur, Croton macrostachyus, and Prunus africana were the least abundant species having only one individual per ha (see Table 1). e species composition and density in each habitat generally depend on the current status of the sites. e seven species-rich families contributed (Asteraceae, Fabaceae, Poaceae, Lamiaceae, Rosaceae, Rubiaceae, and Oleaceae) 49.46% of the total plant species, and the remaining 53 families contributed 50.54% of the total plant species. Twenty endemic plant species were recorded in the study area. Of the 20, 7 herbs, 8 shrubs, 4 tree plant species, and one species were succulent (Table 1).
is showed that Entoto Mountain and its surrounding area are considered as a place with diverse flora including endemic species, and priority should be given to conserve this floristic diversity in the area.
Shannon-Wiener diversity index and species evenness, in the study area, show considerable variation among the land-use types ( Table 2).
Shannon diversity index and species evenness were highest in Riverine vegetation (2.92 and 0.475) followed by natural forest (2.92 and 0.44) and plantation forest (2.85 and 0.385). In contrast, Eucalyptus plantation forest had a Shannon diversity index and evenness of 2.60 and 0.214 followed by degraded land (1.48 and 0.295) which relatively showed lowest species richness. is may be attributed to the impact of Eucalyptus on the growth of other plant species and high disturbance by collection of firewood, animal grazing, and farming. Moreover, some of the areas have been cleared for walking paths. About 63 species (mostly herbs and shrubs) were recorded in Eucalyptus plantation forest and the lowest species richness (15 species) was recorded in degraded land ( Table 3). International Journal of Forestry Research    International Journal of Forestry Research e similarity in species composition of land-use types was above 0.50 except between natural forest and riverine forest (0.35) having low similarity. Comparatively, there was high similarity (0.74) between natural forest and degraded land (Table 3). e total density of woody species in Entoto Mountain and its surrounding area was 3374 stems/ha. In the study area, the highest density of species was recorded for Eucalyptus globulus, which was 952 individuals/ha. e first highest density of naturally regenerated woody species was contributed by Juniperus procera (369 individuals/ha) followed by Carissa spinarum which makes up 304 individuals/ha. e least dense species in the study area were Ficus sur, Millettia ferruginea, Croton macrostachyus, and Prunus africana each contributing 1-6 individuals/ha (Table 4). is may be attributed to the ecological suitability and anthropogenic impacts as the study area is the margin of the capital city of Ethiopia. e frequency gives an approximate indication of the homogeneity and heterogeneity of a stand. e most frequent woody species in the study site was Juniperus procera (90.3%) followed by Eucalyptus globulus and Rosa abyssinica (Table 5).
is may be attributed to the deliberate plantation and natural regeneration of Juniperus procera for the greening of the city of Addis Ababa. Naturally, the area is classified under dry Afromontane forest ecosystem where the Juniper is most dominant. Moreover, Eucalyptus globulus was originally introduced in the 19 th century to solve the problem of fuelwood and construction material for the surrounding people around the Entoto mountain chain.

Regeneration Status in the Study Area.
Composition and density of seedlings and saplings would indicate the status of regeneration in the study area. Information on the regeneration status 72 species was naturally regenerated in the study area. A total of 55 species were represented in the seedling class, and the total seedling density of naturally regenerated woody species was 2876 individuals' ha −1 . e sapling class was composed of 42 species; the total sapling density of naturally regenerated woody species was 3363 individuals' ha −1 . A total of 68 woody species were represented in the mature tree/shrub class and the total mature tree and shrub density was 4645 individuals ha −1 . e result showed that the floristic composition and density of the species were varying; there were seedlings or saplings of Olinia rochetiana, Olea europaea subsp. cuspidata, and Prunus africana while lacked mature woody species. is might suggest that there were exploitations of mature individuals in the study area. e composition, distribution, and density of seedlings and saplings of selected species indicate the future status of the forest. Composition of seedling population perishes before reaching sapling stage due to browsing, grazing, and trampling by wild and domestic animals. Moreover, seedlings are more vulnerable to environmental hazards and biotic factors especially at the early stages of seedling establishment [21]. e ratio of woody species seedlings to mature tree/ shrub (0.62 : 1), seedlings to saplings (0.76 : 1), and saplings to mature tree/shrub (0.81 : 1) showed the distribution of more mature tree/shrub population than that of seedling and saplings. Potential causes of seedling mortality include abiotic stresses such as shade, drought, and trampling, and biotic influences such as herbivory, root competition lack of safe site for seed recruitment, nature of seeds of certain trees which seek dormancy period, litter accumulation, pathogens, species specificity, and moisture stress or probably they might have other alternative adaptations for propagation and reproduction rather than seed germination [21]. erefore, additional work and consecutive monitoring of the natural regeneration in the study area are needed; particularly, the status of soil seed banks has to be investigated to recognize whether or not regeneration potential, other than seedlings and saplings, survives. e plantations have been subjected to natural and human-induced disturbances, which resulted in their degradation or complete destruction. e loss of forest results in soil erosion, land degradation, loss of biodiversity, and impoverishment of ecosystems. In most of the woody plants in dry Afromontane forests, the lack of persistent soil seed banks affects the formation of populations of seedlings on the forest floor [22]. Natural disturbances and human exploitation, such as careful selective cutting, may promote regeneration of the Eucalyptus globulus. However, excessive exploitation of species or clearing and conversion of the forest areas into permanent cultivation will eliminate or reduce the species composition and density especially (degraded land). e absence of soil seed banks and seedlings and removal of mature trees as well as their stumps and roots coupled with poor long-distance dispersal will have severe consequences on the regeneration of the woody vegetation.
is implies that the future existence of tropical dry evergreen Afromontane forests depends on the protection and conservation of the remaining patches of forests [8]. Entoto Mountain and its surrounding area are characterized by high density of naturally regenerated woody species. us, the naturally regenerated woody species are in a good state of regeneration. Juniperus procera, Carissa spinarum, Rosa abyssinica, and Myrsine africana are species with the highest density of naturally regenerating woody plants than the remaining woody species in the study area. Similar findings were reported in the study conducted by Debushe [23]. e probable reason for high density of Rosa abyssinica and Myrsine africana may be due to their resistance to browsing by wild or domestic animals and its low household and economic uses.
Earlier works have shown that the presence or absence of understory vegetation in a plantation is a factor of the density of the stand, the rainfall regime, and management than their origin (reference). Eucalyptus plantations have been existed for centuries without affecting the regeneration potential of some selected species like Rosa abyssinica, Rubus apetalus, Carissa spinarum, Juniperus procera, Maytenus arbutifolia, Maesa lanceolata, Myrsine africana, Laggera tomentosa, Satureja punctata, Dovyalis abyssinica, and Vernonia leopoldi in the study area. is would contribute to the rehabilitation of degraded lands partly by increasing plant biodiversity particularly, shrubs, climbers, and lianas. On top of this, less dense stands of Eucalyptus globulus harbors more regenerated plant species than the high dense stands of Eucalyptus globulus. In fact, human disturbance, such as collection of firewood, animal grazing, farming, and other activities, reduces considerably the regeneration process in Eucalyptus plantation forest.
Many authors [6,7,23] described that Eucalyptus can act as succession catalysts, facilitating the recolonization of native flora through their influence on understory microclimate and soil fertility which is in agreement with the present finding.
A study made by Debushe [23] clearly demonstrated that there is a seed source in the vicinity; establishment of forest plantations can help not only to provide wood for various purposes, rehabilitate degraded lands, and conserve soil and water but also to catalyze natural regeneration of shrubs, climbers and lianas, and some tree species, thereby enhancing plant biodiversity. A study conducted in similar areas is in agreement with the findings of the present study [24].

Conclusion and Recommendations
Entoto Mountain and its surrounding area are characterized by high density of naturally regenerated woody species and relatively in good state of regeneration. From the present study, a total of 179 plant species were recorded and identified. Of which, Asteraceae (30 species) was the most dominant family followed by Fabaceae. Of the total, 77 species were woody plant species. Some of these woody plants which dominantly occur in the study area include Eucalyptus globulus, Eucalyptus camaldulensis, Casuarina equisetifolia, Juniperus procera, and Cupressus lusitanica. In general, the study area provides important economic and social value to the rural communities living around the area, by its attraction to domestic and international tourists. To minimize the present human influence on the area and for the future management of the area in a sustainable manner, conservation and management activities should be immediately implemented by the responsible stakeholders such as Environmental Protection Authority, Ethiopian Biodiversity Institute, Forest Research Center, Forest, Environment and Climate Change Commission, and other institutions working on the related issues. Participatory management programmes should be introduced and implemented to protect locally threatened and the most economically important species from local extinction. Some of the species of conservation concern in the area include Croton macrostachyus, Ficus sur, Ficus vasta, Olea europaea L. subsp. cuspidata, Hagenia abyssinica, Podocarpus falcatus, and Prunus africana.
Raising public awareness on the use, conservation, and management of plant resources and vegetation is very important through extension programmes. Moreover, it is highly required by the responsible bodies to explore indigenous knowledge and other ethnobotanical matters on the diverse uses of plant resources to promote the sustainable use of the plant resources around Mount Entoto and the surroundings of Addis Ababa City.

Data Availability
All the data were obtained from field survey and are open to readers.

Conflicts of Interest
All the authors have declared that there are no conflicts of interest.