Synthesis of Titanium Oxide Nanoparticles Using Root Extract of Kniphofia foliosa as a Template, Characterization, and Its Application on Drug Resistance Bacteria

Biogenic methods of synthesis of nanoparticles (NPs) using plant extracts have been given a great attention due to its nontoxicity and environmental friendliness. In this study, TiO2 NPs were synthesized from titanium tetrabutoxide and extract of root of Kniphofia foliosa. NPs of TiO2 were biosynthesized at different volume compositions of titanium tetrabutoxide to the plant extract with a ratio of 1 : 2, 1 : 1, and 2 : 1, respectively. These green synthesized NPs of TiO2 were characterized by thermogravimetric analysis (TGA/DTA), X-ray diffraction (XRD), scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDS), transmission electron microscopy (TEM), ultraviolet-visible spectroscopy (UV-Vis), and Fourier transform infrared (FTIR) spectroscopy. TGA/DTA analysis has confirmed that the synthesized NPs of TiO2 were stable above the temperature of 500°C. The sharp and intense peaks at 2θ values of 25.3, 38.0, 47.9, 53.2, 54.8862, 62.7, 70.2, and 75.0 have confirmed formation of crystalline NPs of TiO2 in the sample of 1 : 1 and 2 : 1 ratios, and less crystalline samples for TiO2 NPs prepared in a 1 : 2 ratio. Comparison between FT-IR absorption bands of the plant extract and that of calcined NPs of TiO2 confirmed the purity of synthesized nanomaterials, except unavoidable adsorption of moisture on the surface of TiO2 NPs in an open air. The antibacterial activity of biosynthesized TiO2 NPs and that of ethanolic root extract of Kniphofia foliosa was investigated via the disc diffusion method against human pathogen bacteria strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Streptococcus pyogenes. Among the different ratios, TiO2 (1 : 1) NP shows better performance towards Gram-negative bacteria due to its smaller average crystalline size and uniform morphology observed in SEM image relative to the other two ratios of TiO2 NPs. Antibacterial activity of the ethanolic root extract of Kniphofia foliosa itself showed better performance towards Gram-negative bacteria than NPs of TiO2 that might be due to antibacterial activity of residue of ethanol left with the plant extract.


Introduction
The development of reliable experimental protocols for the synthesis of nanoparticles over a range of chemical compositions, sizes, and high monodispersity is one of the challenging issues in the current nanotechnology [1].
to unique properties such as gold and silver are getting synthesized through green method [2,3] for antimicrobial activities. Leaf extracts of Artemisia vulgaris [4] and aerial parts of Callistemon citrinus plant extracts [5] were used in the synthesis of silver NPs for antibacterial and antimalarial applications, respectively. Towards this end, TiO 2 NPs also were more useful in the field of chemistry and nanomedicine as a result of their unique antibacterial and antimicrobial properties as well as their chemical stability. TiO 2 NPs are incorporated in cosmetics whereby creams and ointments are prepared having these nanoparticles to prevent skin aging and sunburns [6]. Successful applications of TiO 2 NPs are due to their increased surface area, which contributes to an increase in surface energy thereby enhancing their microbial and bacterial inhibition. TiO 2 NPs have been found to be useful in treating microbial and bacterial infection diseases; thus, biosynthesis of these nanoparticles could help in dealing with bacterial infections, which have become a menace due to their resistance to available medications [7][8][9]. Several methods have been employed in the synthesis of TiO 2 NPs and other metal and metal oxide NPs which include chemical and physical means with the former being the one mostly practiced industrially. These methods however have their own demerits as they require high temperatures, are potentially hazardous, are not safe to the natural environment, incorporate toxic chemicals as a reducing and capping agent during synthesis process, and at same time, are expensive [10,11].
It has been reported that TiO 2 NPs were produced by the reaction between latex of Jatropha curcas leaves and TiO(OH) 2 . XRD and TEM revealed that the size of synthesized TiO 2 NPs was in the range of 100-200 nm. FTIR spectrum analysis of latex-capped TiO 2 NPs showed the presence of capping/stabilizing agents like protein/peptide material, which also prevents the nanoparticles from agglomeration [12]. It was also reported that TiO 2 NPs were synthesized using aqueous extracts of Eclipta prostrata leaves as a biotemplating agent. The obtained TiO 2 NPs were spherical in shape, their size ranged from 36 nm to 68 nm, and the calculated average crystalline size of biosynthesized TiO 2 NPs was estimated as 49.5 nm [13]. Previously TiO 2 NPs were biosynthesized using rice straw powder as biotemplate with titanium tetraisopropoxide aqueous solution and acetic acid. The average crystalline size of the biosynthesized TiO 2 NPs was in the range of 10-20 nm [14]. Again, TiO 2 NPs were produced using an aqueous extract of Psidium guajava leaf and TiO(OH) 2 precursor. The XRD pattern of the synthesized TiO 2 NPs showed the presence of both anatase (110) and rutile (111) crystallographic structures. The calculated average crystalline size of biosynthesized TiO 2 NPs was found to be 32.58 nm [15]. Plant extracts may act both as reducing and stabilizing agents in the synthesis of TiO 2 nanoparticles and any other metal and metal oxide different NPs. The use of different parts of plants in the biosynthesis of TiO 2 nanoparticles and their applications holds immense potentials towards the environment. The source of the plant extract is known to influence the characteristics of the nanoparticles and hence its applications. This is because different extracts contain different concentrations and different types of organic reducing agents and at same time different capping agents [16][17][18].
The genus Kniphofia (subfamily Asphodeloideae, family Asphodelaceae) comprises 70 species mainly confined to Africa with the center of diversity being South Africa [19], of which, seven occur in Ethiopia; among these seven species, five of them including Kniphofia foliosa, Kniphofia hildebrandtii, Kniphofia isoetifolia, Kniphofia insignis, and Kniphofia schimperi are endemic to Ethiopia [20]. Traditionally, the roots of Kniphofia foliosa have been used for the treatment of different ailments including menstrual pains, infertility, abdominal cramps, wounds, malaria, chest complaint, gonorrhea, and hepatitis [21]. In addition to its traditional medicinal usage for household remedy against various human ailments, the roots of Kniphofia foliosa extracts with various functional groups could also be used for the synthesis of TiO 2 NPs in order to exclude the addition of external stabilizing agents during synthesis process.
Antimicrobial resistance (AMR) is the ability of a microbe to resist the effects of medication that once could successfully treat the microbe. Bacterial resistance occurs as a result of either natural or acquired mechanism. Natural resistance results when the properties of bacteria inhibit the action of a certain antibiotics. As reported earlier, an antibiotic designed to attach to certain specific receptors on a bacterial cell is unable to act if the bacterial species does not have the receptors, while acquired resistance results due to change of bacterial species and its genetic makeup in such a manner that it decreases the action of antibiotics [22,23]. During the last decades, a rapid increment in the development of new antibacterial inorganic and organic materials has been observed as consequence of the spread of antibiotic resistant infection disease, which has become a major issue in the current health care [15,24]. Previously, several different researches were done on the antimicrobial activity of commercially available and chemically synthesized titanium oxide nanoparticles. But those methods are not environmentally friendly and at same time not cost effective. Biosynthesis of TiO 2 NPs using indigenous medicinal plant, root of Kniphofia foliosa extract, for in vitro antibacterial activity was not reported before this work. Therefore, the focus of the present study is biosynthesis of TiO 2 nanoparticles in different ratios using titanium tetrabutoxide as a precursor and Kniphofia foliosa root extract as a reducing and capping agent and then using the different ratios of biosynthesized TiO 2 nanoparticles to investigate its performance on both Gram-positive and Gram-negative human pathogen bacteria strains.

Methodology
2.1. Chemicals. Chemicals, reagents, and solvents used during this work include distilled water, absolute ethanol (99.9%, LabTech Chemicals), titanium tetrabutoxide (98%, Acros Organics), acetone (Sigma-Aldrich), dimethyl sulfoxide (DEMSO, Sigma-Aldrich), and Müller-Hinton agar (Sigma-Aldrich). All these chemicals and reagents are of analytical grade and were used in this work without any further purification.  In each case, the Erlenmeyer flask containing the two components was stirred for about 4 and a half hours without heating. Then, the solution was precipitated by adding a small drop of 1 M sodium hydroxide solution as a precipitating agent. Then, the formed TiO 2 NPs at different volume ratios were allowed to stay within a refrigerator overnight. The formed precipitate for each of the individual ratio was washed with absolute ethanol and distilled water 4 times following with centrifugation at 1000 rpm. At the end of the last centrifugation, the formed TiO 2 NPs were collected using a crucible ceramic dish and placed into a drying oven overnight at 100°C [15]. Then, after conducting the thermal stability of the biosynthesized TiO 2 nanoparticles, it was calcined at 500°C for about 3 and a half hours. Figure 1 shows the systematic biosynthetic procedure of TiO 2 NPs from its precursor (titanium tetrabutoxide) and ethanolic root extract of Kniphofia foliosa as a reducing and capping agent.

Characterization Techniques of Biosynthesized TiO 2 NPs.
Thermal gravimetric analysis (TGA) of the biosynthesized NPs was carried out using a simultaneous differential thermal analysis DTA-TGA (DTG-60H, Shimadzu Co., Japan) and was used to determine the calcination temperature. The crystalline structure and the average crystalline size of the biosynthesized titanium oxide NPs were investigated using an X-ray diffractometer (XRD-7000, Shimadzu Co., Japan) and were recorded with 2θ from 10 to 80 using CuKα (λ = 1:54056 Å) radiation operated at 40 kV and 30 mA. Biosynthesized TiO 2 NPs were characterized by field emission scanning electron microscopy equipped with energy dispersive X-ray spectroscopy (FE-SEM, JEOL-JSM 6500F, made in Japan). The morphology was analyzed using highresolution transmission electron microscopy (HRTEM, Tecnai F20 G2, Philips, Netherlands) at an accelerating voltage of 200 kV. The absorption spectra of the biosynthesized TiO 2 NPs were recorded by using JASCO V-670 UV-Vis spectroscopy equipped with a diffuse reflectance attachment for powder samples in between a wavelength scan of 200 and 800 nm. TiO 2 NPs were characterized and recorded using Fourier transform infrared spectroscopy (FTIR, Perkin Elmer 65) to analyze and detect surface functional groups of the biosynthesized TiO 2 nanoparticles at the scanning range of 4000-400 cm -1 .

Antibacterial Studies
2.5.1. Preparation of Inoculums. Nutrient broth agar of 1.5 g was prepared within 100 mL of DH 2 O and then was placed within four different conical flasks and sterilized. The prepared cultures were subcultured and were inoculated in nutrient broth and were kept on a rotary shaker at 35°C ± 2°C for about 24 hours at 160 rpm.

Inoculation of Test
Plates. Nutrient agar was prepared by taking 6 g nutrient agar and 0.8 g agar-agar followed by dissolving them within 100 mL of deionized water. The prepared agar suspension within 22 minutes was used to inoculate plates by dipping a sterile cotton wool swab into the suspension. Before applying the antibiotic disks, the plates were allowed to dry completely. The plates were incubated at 37°C for about 16-18 hours in an incubator for bacteria and were checked for the zone of inhibition [25].

Disc Diffusion
Method. The antibacterial activity of biosynthesized TiO 2 (1 : 2, 1 : 1, and 2 : 1 ratios) nanoparticles and that of ethanolic crude extract of roots of Kniphofia foliosa was investigated using the disc diffusion method using Müller-Hinton broth agar against both Gram-positive bacteria strains of Staphylococcus aureus and Streptococcus pyogen and Gram-negative bacteria strains of Escherichia coli and Klebsiella pneumonia.
Four bacterial cultures from each bacterial strains were maintained on nutrient Müller-Hinton agar at 37°C, and  3 Journal of Nanomaterials the cultures were kept in appropriate media slants and stored at 4°C (to allow diffusion) until used. The plates were incubated at 37°C for 16-18 h in an incubator and were shaken gently to allow evenly mixing of bacteria cells and agar. Then, 35 mg from each of the three ratios of TiO 2 NPs (1 : 2, 1 : 1, and 2 : 1) was taken and dissolved within 1 mL of DMSO to obtain 35 mg/mL. From each different ratio, 80 μL was taken and saturated with discs (6 mm diameter) and incubated at 37°C for about 24 hours. In antibacterial testing against each strain, triplicate measurements were collected and the average of the triplicates was used in reporting the result.
The antibacterial activity of roots of Kniphofia foliosa extracted using ethanol (ethanol absolute; 99.9%) was investigated using the disc diffusion method. This was done by taking 50 mg of the root powder followed by addition of 25 mL ethanol and then was allowed to stay for about 72 hours until the required crude extract was obtained. The crude was filtered, the solvent was allowed to evaporate, and then, the remaining crude was checked both on Grampositive and Gram-negative human pathogen bacteria strains. The antibacterial activity of both the biosynthesized TiO 2 nanoparticles and the crude extract was evaluated by measuring the diameter (mm) of the inhibition zone around the disc against the test organisms by using a ruler.

Results and Discussion
3.1. Thermal Gravimetric-Differential Thermal Analysis (TGA-DTA). Figure 2 shows TGA-DTA of synthesized TiO 2 NPs. Before calcination process was carried out, the thermal behavior of the as-biosynthesized TiO 2 nanoparticles was analyzed by means of thermogravimetric and differential thermal analysis. TGA curve shows weight loss of the biosynthesized sample whereas DTA curve indicates the energy gain or loss during the process.
Below 150°C, the weight loss was observed due to the removal of physically and chemically entrapped water molecules. In the temperature range of 150-350°C, the weight loss also occurred due to the pyrolysis and carbonization of biomass [1].
Weight loss again continued up to 483°C associated with a strong exothermic peak in the DTA curve, which can be attributed to the vaporization of carbonized residues over the surface of the biosynthesized nanosample. Similar results related to the present study were also reported [14]. After 483°C up to 900°C, no considerable weight loss was observed; therefore, as a result of thermal analysis, 500°C was used as calcination temperature during this work.
3.2. X-Ray Diffraction (XRD) Analysis. Figure 3 shows XRD of TiO 2 NPs synthesized within three different volume ratios. The formation of biosynthesized TiO 2 NPs was also confirmed by X-ray diffraction measurements. The diffraction peaks were observed at 2θ values of ≈25.3, 38.0, 47.9, 53.2, 54.8862, 62.7, 70.2, and 75.0 along with their Miller index planes of (101), (004), (200), (105), (211), (204), (220), and (215), respectively. The analysis confirms that the biosynthesized titanium oxide nanoparticles are in a tetragonal crystal structure without any impurities within the detection limits of XRD instrument and within the scanned region done from 10°to 80°. As the width of the peak increases, the size of the particle decreases, which resembles that the present biosynthesized TiO 2 material is within the range of nano [26].
The average crystalline size for the different kinds of TiO 2 NPs was estimated as 10.2, 8.2, and 8.5 nm for the 1 : 2, 1 : 1, and 2 : 1 ratios, respectively. The average crystallite size of TiO 2 nanoparticles synthesized within a 1 : 1 ratio has a relatively smaller particle size of 8.2 nm as compared to the average crystalline size of 8.5 nm for the case of a 2 : 1 ratio. This is due to the fact that the greater amount of the extract used during the synthesis process results in more capping agents/stabilizing agents that in turn effectively stabilize the biosynthesized TiO 2 nanoparticles during the synthesis process. The peaks of both of the XRD spectra are in good agreement with the literature reported before (19). As it can be observed for the XRD spectrum (a), TiO 2 (1 : 2) NPs losses  Journal of Nanomaterials their crystalline nature due to the addition of an excessive amount of the root extract of Kniphofia foliosa beyond the coating surface of TiO 2 NPs.

Scanning Electron Microscopy-Energy Dispersive
Spectroscopy (SEM-EDS). Figure 4 shows SEM images of TiO 2 NPs synthesized within three different volume ratios. SEM images revealed that the biosynthesized TiO 2 NPs were found to be spherical in shape with distinct edges. SEM images also revealed the increase of particle size with the increase of ethanolic root extract of Kniphofia foliosa. Moreover, the uniformity of the SEM image in case of TiO 2 (1 : 1 and 2 : 1) relative to TiO 2 (1 : 2) ratio implies the well association of biomolecules obtained from the root extract with TiO 2 nanoparticles during the synthesis process; and the presence of the root extract coats the surface of the TiO 2 nanoparticles thus preventing from aggregation. As it can be shown from Figure 4(a), the particles show agglomeration which results due to the presence of excess amount of root extract of Kniphofia foliosa, which was beyond the coating surface of TiO 2 NPs [27]. This image was observed within the magnification of 20 μm.
To gain a further insight into the features of the biosynthesized TiO 2 nanoparticles, the analysis was performed using EDS techniques. The absence of any foreign materials other than the required elements (titanium and oxygen) indicates the elimination of water molecules, ethanol molecules, and other organic residues during the centrifugation, drying, and calcination steps; this reveals that the synthesized TiO 2 NPs were pure as supported by the XRD analysis results [28].
EDS analysis results ( Figure 5) clearly showed that the peaks due toTi were highly intense, and at the same time, the result showed low intense oxygen peaks which may be due to the dissociation of the precursor compound (titanium tetrabutoxide) used during the synthesis of process. EDS also revealed the formation of nonstoichiometry TiO 2 NPs with oxygen vacancy, which leads to the better performance for the desired application (in the present case for antibacterial activity). Figure 6 revealed TEM image, SAED pattern, and HRTME results of the biosynthesized TiO 2 (1 : 1) NPs. TEM analysis was carried out to ascertain and gain further information 10    5 Journal of Nanomaterials about the biosynthesized TiO 2 NPs. TEM image exhibits that the morphology of the biosynthesized titanium oxide nanoparticles possesses a spherical/sphere-like structure in shape without any agglomeration.
The image in Figure 6(c) with higher magnification shows the high-resolution transmission electron microscope (HRTEM) of anatase TiO 2 NPs. The particles with a fringe width of 0.357 nm were confirmed to be anatase form of biosynthesized TiO 2 (101) nanoparticles. The lattice fringes also clearly indicate that the particles are nanocrystalline with an anatase phase form, which is also confirmed by the XRD analysis result and from the SAED pattern analysis. Figure 7 shows the UV-Vis absorption spectrum and the Tauc plot spectrum for the different kinds of biosynthesized TiO 2 NPs. The absorption spectra of the biosynthesized TiO 2 NPs reveal the reduc-tion process and formation of TiO 2 , which shows excellent agreement with those reported in literatures previously. The bandgap energy was determined based on the numerical derivative of the optical absorption coefficient using Tauc's plot method and was found to be 3.34, 3.32, and 3.37 eV for the 1 : 2, 1 : 1, and 2 : 1 volume ratios of Ti precursor salt and root extract, respectively. The variation in the E g for the different kinds of biosynthesized TiO 2 NPs is due to the variation in volume ratio between Ti precursor salt and the root extract that leads the biosynthesized TiO 2 NPs to absorb at different regions of UV-Vis light.

Ultraviolet-Visible (UV-Vis) Analysis.
Broadening of the spectrum indicates the polydispersed nature of the biosynthesized nanoparticles and the red shift of the absorption curve results in the reduction of the bandgap energy.  Journal of Nanomaterials vibration on phenolic compound, C-O bond stretching of the hydroxyl group, and out of plane C-H bending at aromatic ring, respectively, indicating and showing the presence of organic compounds such as knipholone anthrone, anthraquinone, and chrysophanol [29]. In Figure 8(b), the broad absorption band observed at 3433.85 cm -1 represents O-H bond stretching due to adsorbed moisture at the surface of TiO 2 NPs, and the weak absorption band at 2335.14 cm -1 is due to C=O bond stretching that could be emanated from the presence of adsorbed carbon dioxide on the surface of NPs. The absorption band at 1629.58 cm -1 also was due to carbon dioxide and/or due to O-H bending of molecularly adsorbed water on the surface of the synthesized material shown in Figure 8(b) [30].
The broad band centered at 567.13 cm -1 represents a characteristic peak of Ti-O-Ti bending mode of vibration which confirms the formation of metal oxygen bonding [31,32]. The absence of bands at 2926.88, 1635.14, 1319.59, 1038, and 780.44 cm -1 in Figure 8(b) shows that organic molecules used during synthesis have been removed from the TiO 2 NPs upon calcination above 500°C.
3.7. Antibacterial Study Analysis. The antibacterial activity of the biosynthesized TiO 2 nanoparticles and that of the ethanolic crude extract of the roots of Kniphofia foliosa was investigated using the disc diffusion method. Table 1 shows the antibacterial activity of TiO 2 nanoparticles biosynthesized within 1 : 2, 1 : 1, and 2 : 1 ratios, which shows the antibacterial zone of inhibition between 8 and 6 mm at the concentration of 35 mg/mL against Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Streptococcus pyogenes.
Among the different ratios of the biosynthesized TiO 2 NPs, TiO 2 (1 : 1) NPs show better performing antibacterial activity as compared to the remaining two ratios. This might be due to the smaller average crystalline size; and in addition to this, the enhancement is also due to the more uniform spherical shape of TiO 2 NPs as it can be seen from its SEM image. From the present study, it is clearly evident that the greater inhibitory action of green synthesized TiO 2 (1 : 1) nanoparticles depends not only on average crystalline size of the nanoparticles but also on the difference in volume of capping agents obtained from the root extract of Kniphofia foliosa. Biosynthesized TiO 2 NPs within a volume ratio of 1 : 2 show resistant towards S. aureus, S. pyogenes, and K. pneumonia bacteria strains except E. coli. This might be because of that the morphology of the biosynthesized TiO 2 NPs shows agglomeration which results in lowering the in vitro antibacterial activity. This might also be due to the larger average crystalline nature of the formed nanoparticles in its XRD spectrum as compared to the other ratios.
The antibacterial activity of the crude ethanolic root extract of Kniphofia foliosa was investigated by measuring   the zone of inhibition and found to be 10, 10, 6, and 6 mm for the S. aureus, S. pyogenes, E. coli, and K. pneumonia, respectively. The crude ethanolic root extract of Kniphofia foliosa shows the best antibacterial activity against Gram-positive bacteria (S. aureus and S. pyogenes). This indicates that the ethanolic crude extract of the plant was more sensitive against Gram-positive bacteria than Gram-negative bacteria [27]. The zones of inhibition by ethanolic root extract of Kniphofia foliosa against Gram-negative bacteria were smaller than that of Gram-positive bacteria. This is because Gramnegative bacteria possess additional external cell membrane/double cell membrane that allows them to resist.
Green synthesized TiO 2 NPs show antibacterial activity because biomolecules obtained from the root extract of Kniphofia foliosa are giving excess electron to TiO 2 and result in the formation of superoxide radicals O 2 -, and the superoxide radicals produce reactive oxygen species (ROS) in bacterial cell. That ROS is used to break the bacterial cell membrane. As the superoxide radical production increased, ROS production also increased. These are all of the reasons for electron production from TiO 2 nanoparticles, and as a result, TiO 2 NPs show antibacterial activity against both Gram-negative and Gram-positive bacteria strains [33].
According to several studies, it is believed that metal oxides carry positive charge while bacteria carry negative charges; this causes electrostatic attraction between bacterial cells and metal oxide NPs, which leads to oxidization and finally death of microorganisms [33]. Nanomaterials also could deactivate the cellular enzymes and DNA by coordinating to the electron-donating group. Therefore, this result showed that TiO 2 nanoparticles were effective for inhibiting Gram-positive and Gram-negative bacteria. As it can be observed from Table 1, the antibacterial activity of the synthesized TiO 2 NPs towards Gram-positive bacteria strains is low. This might be due to the nature of the bacteria strains that resist towards the synthesized material. The antibacterial effect of biosynthesized TiO 2 nanoparticles may be due to its small size, more uniform spherical shape, and more active sites of TiO 2 NPs. This also implies that TiO 2 NPs may destroy the outer membrane of the bacterial cell, directly leading to the leakage of minerals, proteins, and genetic materials, causing cell death of the corresponding human pathogen bacteria.

Conclusion
In this work, TiO 2 NPs were successfully biosynthesized using titanium tetrabutoxide as a precursor in the presence of Kniphofia foliosa root extract within different ratios. More crystalline nature and best performing TiO 2 NPs were obtained when it was biosynthesized within a 1 : 1 ratio. The biosynthesized TiO 2 NPs were characterized using different instruments. The particles were found to be thermally stable above 500°C. Crystalline analysis showed the average crystalline size as 10.2, 8.2, and 8.5 nm for 1 : 2, 1 : 1, and 2 : 1, respectively. SEM reveals the morphology as spherical, and EDS analysis also indicates presence of the elements such as Ti and O only. TEM image confirmed the spherical morphology of the biosynthesized TiO 2 NPs, and HRTEM analysis proves that the lattice spacing of 0.357 nm confirms (101) plane of anatase TiO 2 NPs. The bandgap was calculated in the range of 3.32-3.37 eV. Functional group analysis indicates the presence of various capping and stabilizing agents. The in vitro antibacterial activity of both the biosynthesized three ratios of TiO 2 NPs and ethanolic crude root extract of Kniphofia foliosa was investigated. Among the different ratios of TiO 2 NPs, TiO 2 (1 : 1) have better performing antibacterial activity. This is due to its small average crystalline size, uniform spherical shape, and large surface region, which creates electronic effects, and these effects can increase the binding strength of the nanoparticles with the bacteria cell membrane. Ethanolic crude root extract of Kniphofia foliosa has a better activity against S. aureus and S. pyogenes.

Data Availability
The data of UV-Visible absorption spectra, FTIR spectra, XRD Analysis, SEM, EDS, TEM images, HRTEM images, SAED patters, and antibacterial activity used to support the findings of this study are included within the article; and also can be released from corresponding author upon application to the Review Board of Hindawi (Journal of Nanomaterials).

Disclosure
The funder, Adama Science and Technology University, has not been involved in the editing, approval, or decision to publish this manuscript.