Novelty of Bioengineered Iron Nanoparticles in Nanocoated Surgical Cotton: A Green Chemistry

The current focus of nanotechnology is to develop environmentally safe methodologies for the formulation of nanoparticles. The phytochemistry of Zingiber officinale inspired us to utilize it for the synthesis of iron nanoparticles. GC-MS analysis revealed the phytochemical profile of ginger. Out of 20 different chemicals, gingerol was found to be the most potent phytochemical with a retention time of 40.48 min. The present study reports a rapid synthesis method for the formation of iron nanoparticles and its potential efficacy as an antibacterial agent and an antioxidant. Because of its antibacterial property, ginger extract was used to coat surgical cotton. Synthesized ginger root iron nanoparticles (GR-FeNPs) were characterized by UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction analysis, and particle size analysis. XRD confirmed the crystalline structure of iron oxide nanoparticles as it showed the crystal plane (2 2 0), (3 1 1), (2 2 2), and (4 0 0). The particle size analyzer (PSA) showed the average size of the particles, 56.2 nm. The antimicrobial activity of the FeNPs was tested against different Gram-positive and Gram-negative bacteria. E. coli showed maximum inhibition as compared with the other organisms. Antioxidant activity proved the maximum rate of free radicals at 160 µg/mL produced by nanoparticles. In addition, the antimicrobial activity of nanocoated surgical cotton was evaluated on the first day and 30th day after coating, which clearly showed excellent growth inhibition of organisms, setting a new path in the field of medical microbiology. Hence, iron-nanocoated surgical cotton synthesized using green chemistry, which is antimicrobial and cost effective, might be economically helpful and provide insights to the medical field, replacing conventional wound healing treatments, for better prognosis.


Introduction
In the modern era, it is crucial to find a substitute for conventional antibiotics because of the emergence of new multidrug-resistant bacterial strains that are able to form biofilms, decreasing the action of antibiotics [1].e recent advancements in the field of nanotechnology include the preparation of nanoparticles of specific size and shape that exhibit antimicrobial properties.e antimicrobial activity of the nanoparticles can be determined by the size, physicochemical properties, and surface area-to-volume ratio [2][3][4][5].It is reported that nanoparticles of smaller size tend to exhibit excellent antimicrobial activity.Various polyphenols and antioxidants present in the Z. officinale root play an important role in the field of medicine, and interaction of these with the metallic surface of the nanoparticles exhibits a possible antioxidant activity [4].Because of the growing concerns of society regarding health issues, consumers are paying attention before they pick any product.at is the reason for the demand of antimicrobial agents in the market.
e essential oil of Z. officinale has antimicrobial, antioxidant, antifungal, insecticidal, and anti-inflammatory properties [5].It created a special interest for choosing ginger as a plant for the preparation of iron nanoparticles.Various chemical and biological methods can be used to prepare nanoparticles, but synthesis via a green approach using Z. officinale root extract is ecofriendly, cost effective, easy, and less hazardous [6][7][8].
In comparison with the previous reports, few studies have reported on the synthesis of iron oxide nanoparticles from Z. officinale and its antimicrobial evaluation on surgical cotton.Iron is a cost-effective alternative compared with other expensive metals reported earlier as antimicrobial agents.is study's focus is to synthesize iron nanoparticles (FeNPs) from Z. officinale; confirm the formation of FeNPs by different characterization methods such as UV-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction; check the bactericidal activity of FeNPs; and coat the FeNPs on surgical cotton.Because of antibiotic-resistant bacterial strains, wound dressing of patients is difficult.us, the study may provide insight into a new path which may be an alternative for antibiotic use in the near future.

Materials and Reagents.
All the chemicals including ferric chloride [FeCl 3 ], isopropyl alcohol, DPPH [2, 2diphenyl-1-picrylhydrazyl], ascorbic acid, methanol, and antibiotic disks were of analytical reagent grade and used directly without any further purification.Ingredients for media preparation were from HiMedia.Ginger was collected from a local market in Gandhidham, Gujarat, India.Distilled water was used in all experiments.

GC-MS Analysis of Zingiber officinale Root Extract.
e root extract of Z. officinale was analyzed using HP5 Agilent Technology 5977B (Santa Clara, US) model no.7820A MS coupled to a 5977B equipped with a HP-5 fused silica capillary column (30 m × 0.320 mm × 0.25 μm film thickness).Helium gas was used as the carrier gas.GC-MS programme was set as per the method described by Dhalani et al. [9].

Preparation of the Plant Extract.
Ginger was collected from the local market and washed thoroughly with distilled water to eliminate dust on the surface, chopped into small pieces, sundried, and powdered.e extract was prepared by mixing 12 grams of dried ginger powder in 200 mL of isopropyl alcohol, and the mixture was stirred on a magnetic stirrer at 80 °C for 1h; thereafter, the extract was filtered carefully, and the supernatant collected and stored at room temperature for further use [9].

Green Synthesis of Iron
Nanoparticles.GR-FeNPs were synthesized by adding the equivalent extract to 0.01 M•FeCl 3 at room temperature and constantly stirring for 10 min.Immediate appearance of black brown color showed the reduction of Fe +3 ions, which is the first indication of the formation of iron nanoparticles.Afterwards, the liquid was poured into large Petri plates and dried at 100 °C for 24 h in a hot-air oven and cooled down the next day.e upper layer of the plate was scraped out carefully using a spatula.e fine-dried black powder of ginger iron nanoparticles was kept ready for further characterization.All nanoparticle preparations were performed according to our previous study [10,11].
2.5.Characterization 2.5.1.UV Visible Spectroscopy.UV visible spectroscopic analysis of the synthesized FeNPs was done by using 0.1 ml of sample diluted in 2 ml of deionized water.Absorbance was measured with the help of ABTRONICS Model No. LT-2900 in the range of 200-700 nm [12,13].(Fourier Transform Infrared Spectrophotometer).FT-IR spectra of dried FeNPs and plant extract were determined using a Fourier transform infrared spectroscope.

FT-IR Analysis
e synthesized nanoparticles were lyophilized and mixed with KBr pellets and further processed.An average of 9 scans were collected for each measurement with a resolution of 4 cm −1 at a range of 4000-650 cm −1 [14].

Particle Size Analyzer (PSA).
e synthesized nanoparticles were analyzed by using a particle size analyzer, which measures the particle size by its flow through a beam of light producing a size distribution from the smallest to largest dimensions.When the particles are dissolved in water, they stay in a colloidal form and flow with a velocity that depends on their size and zeta potential (Brownian movement) [15].

X-Ray Crystallography.
e crystalline structure of the synthesized nanoparticles was analyzed by powder X-ray crystallography (XRD).

Antimicrobial Activity. Gram-negative bacterial strains such as Escherichia coli MCC 2246 and Klebsiella pneumoniae MCC 2716 and Gram-positive strains such as
Staphylococcus aureus MCC 2408 and Bacillus subtilis MCC 2244 were cultured overnight in nutrient broth media at 37 °C with continuous agitation on an orbital shaker platform at 180 rpm.Simultaneously, nutrient agar media was dispersed into sterile petri plates and incubated for 24 hours at 37 °C for sterility check.e overnight broth cultures of the test organisms (E.coli, K. pneumoniae, S. aureus, and B. subtilis) were used as inoculum.Antimicrobial activity was analyzed using the agar well diffusion method.
e test culture of each organism (100 μl) was applied to each plate.

Antimicrobial Activity of Iron Nanoparticle-Coated
Surgical Cotton.FeNPs (30 µg/ml) were dissolved in methanol and sonicated for 10 minutes in an ultra sonicator.Dip coating is the precision-controlled immersion and withdrawal of a substrate into a reservoir of liquid for the deposition of a layer of material over it.A cotton piece of size 0.5 cm × 0.5 cm (substrate) was fixed to the head portion of the dip coater, which has a mechanical body that moves up and down.A small beaker with diluted NPs sample is placed 2 Advances in Pharmacological Sciences below the body, and the dip coating process was performed three times at a particular pressure and speed.e coated cotton was carefully removed using sterile forceps, dried using an air dryer, and stored in a ziplock bag for further use.Antimicrobial activity was analyzed using the agar well diffusion method.100 μl of the test culture for each microorganism was spread using a sterile glass spreader on the nutrient agar plate and left for 10 minutes for the inoculum to get absorbed into the agar medium.A small piece of FeNPs-coated surgical cotton was placed in the middle of the plate and incubated for 48 hours, and the results noted [19,20].(1)

GC-MS Analysis of Zingiber officinale Root Extract.
e GC-MS profile of Z. officinale root extract is shown in Figure 1. e retention time and area (%) of each compound are given in Table 1.Out of 20 different chemical compounds, gingerol was found to be the major component with the highest retention time of 40.368 min.e presence of gingerol might be the reason for the reduction of metal ions and bactericidal activity of the nanoparticles.

Mechanism of FeNPs Formation from Z. officinale Extract.
e process of synthesizing nanoparticles from plant extracts has been proven to be one of the most reliable, nontoxic, and eco-friendly approaches towards green chemistry and plant biotechnology.Plant extract is mixed with 0.01 M•FeCl 3 at a ratio of 2:3.e color change is due to the presence of polyphenols present in the plant extract which act as a reducing and capping agent, lowering the valency of Fe +3 to Fe 0 , as shown in Figure 2.
Earlier studies reported that the color transformation from yellow to reddish black is the primary indication of the formation of iron oxide nanoparticles [24].e aldehyde and polyphenol groups present in the leaf extract are responsible for the reduction of ferric chloride [12,25].

UV-Visible Spectroscopy (UV-Vis).
Spectroscopy is an analytical technique concerned with the measurement of absorption of electromagnetic radiation.UV-Vis spectroscopy is one of the oldest methods in molecular spectroscopy.It refers to absorption spectroscopy in the UV-visible spectral region.is means it uses light in the visible and adjacent ranges.
e absorption varies with the difference in color in the chemicals in the given samples.e UV-visible spectra of iron nanoparticles in the aqueous ginger extract are shown in Figure 3.
e absorption peak at a wavelength between 200 and 260 nm indicates the formation of iron nanoparticles (Figure 3).e sharp and intense peak is attributed to the uniform size of the particles [26,27].

FT-IR Analysis.
FT-IR analysis was carried out to evaluate the possible interaction between the biomolecules and Fe 3+ during the biogenic reduction reaction.e FT-IR data for FeNPs containing Z. officinale root extract are given in Table 2. e bond stretching at 2927.8 cm −1 is attributed to the C-H bond, 1638.3 cm −1 to the C�O bond, 1517 cm −1 to the C-C amide group at 861 cm −1 , and C-N to 1075.3 cm −1 and 760 cm −1 , which was found to be very close to 688 cm −1 , which was attributed to the presence of zero valent FeNPs as shown in Figure 4. We can observe the FT-IR data of FeNPs with a plant sample [Figure 4] by analyzing the shift in bond stretching of the C-H bond from 2922 cm −1 to 2927 cm −1 , C-C bond from 1514 cm −1 to 1517 cm −1 , and C-N bond from 1037 cm −1 to 1075.5 cm −1 .

Particle Size Analysis.
It is evident from the particle size analysis that smaller nanoparticles below 100 nm were synthesized, which might have agglomerated and resulted in larger nanoparticles.Furthermore, because of the magnetic property, the particles might have agglomerated, producing larger dimensions as depicted in Figure 5.

Antimicrobial Activity. Various Gram-positive and
Gram-negative bacterial strains were used to check the bactericidal activity of FeNPs synthesized via green chemistry.e excessive use of antibiotics has led to the emergence of new multidrug-resistant strains.It is necessary to find an alternative to antibiotics.Earlier studies reported the use of expensive metal nanoparticles as antimicrobial agents Advances in Pharmacological Sciences [31][32][33].To overcome this problem, the current study's focus was to design an eco-friendly and cost-e ective method.e results are shown in Table 3. E. coli and K. pneumoniae show more sensitivity than Gram-positive bacteria.Compared with Gram-positive bacteria, Gram-negative bacteria have a thin layer of peptidoglycan.Hence, FeNPs can easily    Advances in Pharmacological Sciences penetrate the cell wall of Gram-negative bacteria (Figure 7).Our results support the notion of earlier studies that E. coli and K. pneumoniae showed higher zones of inhibition than B. subtilis and S. aureus [26,27,34].

Antimicrobial Activity of Iron Nanoparticle-Coated Surgical Cotton.
e key outcome of the present study is the FeNPs-coated surgical cotton.e bactericidal activity of FeNPs extended to the surgical cotton, and it can be used further in wound healing, tissue therapy, and other medicinal applications.10 μg/ml of FeNPs was used to coat the surgical cotton with the help of a dip coater.e antimicrobial activity was evaluated on Gram-positive B. subtilis, S. aureus, and Gram-negative E. coli, by the standard disc di usion method.e results are given in Table 4. e results showed the radial diameter of the inhibiting zones of B. subtilis, S. aureus, and E. coli after 24 hours.e clear inhibition zones made by the FeNPs-coated surgical cotton obtained in the present study are shown in Figure 8. Antimicrobial activity was evaluated on day zero and 30 days after coating.Initially, the particles showed higher  Advances in Pharmacological Sciences antimicrobial activity, which diminished in terms of zone diameter due to the development of resistance in the microbial culture used for the study.e clear zone even after 30 days indicates bacterial growth restriction by the di used FeNPs over the surgical cotton.Furthermore, the green approach for synthesis of FeNPs can be applied on cotton fabric which could have good bactericidal activity in wound dressing [30,[35][36][37][38].    us, the function of an antioxidant system is not to remove oxidants entirely but instead to maintain an optimum level inside the body.Ascorbic acid has high amounts of antioxidants; thus, it was used as standard (Figure 9).By using the DPPH assay at different sets of concentrations, antimicrobial activity was evaluated in triplicate.e total antioxidant capacity of Z. officinale was expressed as the number of equivalents of ascorbic acid.e color of the DPPH solution in the presence of the GR-FeNPs changes gradually from deep violet to pale yellow, which allowed the visual monitoring of the antioxidant activity of the nanoparticles.e observed effect of FeNPs is in the following order: ascorbic acid > FeNPs > FeCl 3 > plant extract [Figure 8].
e study revealed that the antioxidant activity of the extract follows an increasing trend with the increase in concentration of the GR-FeNPs.DPPH activity results showed the highest free radical % scavenging potentials of 0.01 M FeCl 3 , GR extract, GR-Fe NPs, and ascorbic acid to be 74%, 71%, 89%, and 92%, respectively, at a concentration of 160 μg/mL [39,40].

Conclusion
e present work highlighted the green chemistry of synthesizing iron nanoparticles from the root extract of Z. o cinale.e production proved to be easy, cost e ective, and eco-friendly with natural reagents and less harsh chemicals.e color change was also remarkable when ferric chloride solution was mixed with the reducing agent of the plant extract.e biosynthesized FeNPs were characterized by UV-Vis spectroscopy that showed a surface plasmon resonance behaviour.e antimicrobial activity reported using green approach-synthesized nanoparticles may be further bene cial for various applications for better prognosis of several diseases, and the antioxidant activity of Z. o cinale root extract has shown tremendous results.Iron nanocoated surgical cotton would have great role for distinguished health applications by creating new gadgets such as biosensors which can be implemented for the study may enhance the e ects of conventional antimicrobials, which will probably decrease costs and improve the treatment quality.us, nanocoated surgical cotton obtained using a green synthesis approach might be a promising path in the eld of medical microbiology.Future studies are still needed to design nanochips having an antimicrobial property which can replace antibiotics in our lives.

Figure 2 :
Figure 2: Primary indication of formation of nanoparticles-color change from yellow to brown.

Table 1 :
Phytochemical GC-MS analysis of Zingiber o cinale root extract.

Table 2 :
Comparison of bond stretching of synthesized FeNPs with Z. o cinale root extract.

Table 3 :
Diameter of the zone of inhibition of GR-FeNPs against Gram-positive and Gram-negative bacteria.