Spectrum-Effect Relationship between UPLC Fingerprints and Antilung Cancer Effect of Si Jun Zi Tang

Nowadays, an increasing number of patients are seriously affected by lung cancer. Si Jun Zi Tang (SJZ), a four-herb Chinese medicine formula first described approximately one thousand years ago, is often prescribed for cancer patients as a complementary therapy. But the research on the effective materials for treating cancer using SJZ was rarely reported. To solve this problem, we evaluate the inhibitory effect of 10 samples of SJZ from different origins on PC9 cells. Ultraperformance liquid chromatography (UPLC) and hierarchical cluster analysis (HCA) along with canonical correlation analysis (CCA) and bioactivity validation were used to investigate the underlying correlation between the chemical ingredients and the antiproliferative effect of SJZ on PC9 cells. The evaluation indicated that 10 batches of SJZ could inhibit proliferation of PC9 cells and there was a notable difference in pharmacological activity between the different SJZ samples. The results of CCA and multivariate statistical analysis indicated that ginsenoside Ro and ginsenoside Rg1 might be active constituents of the antiproliferative effect as determined by spectrum-effect relationships. The results showed that bioassay and spectrum-effect relationships are suitable to associate sample quality with the active ingredient associated with clinical efficacy. And our finding would provide foundation and further understanding of the quality evaluation of traditional Chinese medicine decoction.


Introduction
Lung cancer is the leading cause of cancer-related mortality worldwide. 1.8 million people are diagnosed with lung cancer, and 1.6 million people die from this disease every year [1,2]. Malignant proliferation of tumor cells is one of the leading causes of death. Traditional Chinese medicines (TCMs) have been used to treat a variety of tumor with the obvious advantages of fewer side effects [3,4]. Si Jun Zi Tang (SJZ) was first described in the "Prescriptions People's Welfare Pharmacy" about a thousand years ago and consisted of Panax ginseng C.A.Mey., Atractylodes macrocephala Koidz., Poria cocos (Schw.) Wolf., and Glycyrrhiza uralensis Fisch. at a proportion of 3 : 3 : 3 : 2. As a representative formula for tonic TCM, SJZ has the effect of replenishing qi and strengthening spleen and is usually used to regulate gastrointestinal function, anti-inflammatory, and enhances immunity [5,6]. Furthermore, it has been found that SJZ played a crucial role in preventing tumor recurrence and metastasis, improving quality of life, and prolonging the overall survival time, across a range of lung cancer, colorectal cancer, liver cancer, and gastric cancer [7][8][9][10][11]. Our previous research has shown that SJZ significantly suppresses Lewis lung cancer (LLC) growth in LLC-bearing mice [12]. However, the chemical composition of SJZ against lung cancer remains unclear, and it is difficult to control the quality linked to the clinical effects.
UPLC fingerprint is a useful tool for identifying authenticity, assessing quality, and distinguishing the ingredients of TCMs [13].
e spectrum-effect relationship that determines the correlations between fingerprint and biological activity is a scientific method for elucidating the pharmacodynamic basis and establishing a method for controlling the quality of TCMs [14][15][16][17].
Here, we evaluated the effect of SJZ on PC9 cells by a spectral-effect relationship approach. e IC50 of SJZ on PC9 cells was determined by analyzing ten batches of SJZ from different geographic regions. UPLC was performed to generate fingerprints of SJZ. Furthermore, UPLC and correlation analysis statistical method were used in combination to elucidate the relationship between pharmacological activity and chemical properties of 10 SJZ samples for characterizing their spectrum-effect relationships.

Plant Sample Preparation.
e plant material of R, B, F, and G was ground into powder using a mill. 2.2 g SJZ powder consisted of R, B, F, and G powder (ratio 3 : 3 : 3 : 2) and were dissolved in 20 mL distilled water. e mixture was vortexed and heated to boil and kept faint boiling for 1 h. e sample was cooled, centrifuged for ten minutes at 3000 rpm, and then concentrated by a rotary evaporator.

Preparation of Reference Standard Solution.
Standard solutions were prepared by adding accurately weighed amounts of ginsenoside Rg1, Re, Rb1, Rc, Ro, and Rd, liquiritin, ammonium glycyrrhetate, and atractylenolide III dissolving with methanol to produce stock solution and stored at 4°C. Stock solutions of all the standards were diluted to the required concentrations and then mixed immediately before analyses. e final concentrations were 84.  2.6. Cell and Cell Culture. PC9 cells were cultured in the DMEM high-sugar medium supplemented with 10% fetal bovine serum, penicillin (100 U/mL), and streptomycin (100 μg/mL) at 37°C under a humidified atmosphere containing 5% CO 2 .

Cells Viability
Assay. All ten water extracts of SJZ were dissolved in the 1 mL DMEM high-sugar medium. After incubation under 5% CO 2 at 37°C for 24 h, PC9 cells (10 4 cells per well) were subsequently inoculated onto 96well plates and cultured for 24 h before onset of treatment. en, the supernatant was discarded, and cells were treated with SJZ samples over a broad dose range to establish growth curves for 48 h. After that, cells were incubated for an additional 1 h with the CCK-8 reagent (100 μL/mL medium). e absorbance was determined at 450 nm wavelength. e experiments were performed in triplicate. e proliferative inhibition rate was measured using the following formula: 2 Evidence-Based Complementary and Alternative Medicine e IC50 (50% inhibitory concentration) value was calculated by nonlinear regression analysis using Graph-Pad Prism software (San Diego, CA, USA).

HCA.
HCA is a multivariate analysis method that sorts specimens into clusters. is analysis can divide objects into specific clusters by means of maximizing homogeneity within each cluster while also maximizing heterogeneity between cluster [19,20]. In this study, HCA was used to assess correlations in the UPLC fingerprints of 10 SJZ samples and was performed with Metabo Analyst 3.0 (http://www.metaboanalyst.ca/).

2.9.
CCA. CCA is to study the correlation between two continuous variables, extracting the main principal components of them and finding their linear combinations of two sets of variables. Pearson's correlation coefficient is used to quantify the degree of colocalization between paired data. In our study, CCA was used to analyze the relevance between the peak area values from the UPLC fingerprints and IC50 from the measurement of inhibition on PC9 cells using SPSS statistics software (SPSS for Windows 17.0, SPSS Inc., USA).

Effect of SJZ on PC9 Cell
Viability. CCK-8 assays were performed to investigate the effects of SJZ on the proliferation of PC9 cells. After treatment for 48 h with different concentrations, all ten batches of SJZ exhibited growthinhibitory effects against PC9 cells which was dose-dependent (Figures 1(a)-1(j)). Values of IC50, the concentration of the extract required to inhibit cell growth by 50% of the control level, were estimated from the corresponding concentration and growth-inhibition plot. e bioactivities of samples showed various IC50 values for PC9 cells, from 0.48 to 35.95 mg/mL (Figure 1(k)). It seemed that the growth-inhibitory effect of the SJZ1 was strongest as it had the lowest IC50.

Analysis of UPLC Fingerprints.
Ten SJZ samples were analyzed by UPLC-UV. e fingerprints of their UV chromatograms are shown in Figure 2. Under optimized conditions, chromatograms were generated for all batches of SJZ (Figure 2(c)) as well as for a mixture of reference substances (Figure 2(a)) and a test sample of SJZ4 (Figure 2(b)). e professional similarities were calculated using the Similarity Evaluation System for Chromatographic Fingerprint of Herbal Medicines (Version 2012.130723). Similarities between the reference fingerprint and each chromatographic profile of the ten batches of SJZ samples were evaluated by calculating the correlation coefficient.
ere were similar chemical profiles across different batches, and nine common peaks were found between 1 and 30 min time intervals by comparing ultraviolet spectra and UPLC retention times for the ten chromatograms. By comparing with the fingerprints of the reference substances, nine common peaks (a, b, c, d, e, f, g, h and i) were identified as liquiritin, ginsenoside Rg1, Re, Rb1, Rc, Ro, and Rd, ammonium glycyrrhetate, and atractylenolide III. And the highest peak in the fingerprints was peak a. As shown in Table 1, some differences were noted among data collected for the common peaks, such that the coefficients of variance (RSD%) for all common peaks were larger than 34.92%, which indicated that the content of chemical constituents was variant in different batches of SJZ from different origins.

Results of Hierarchical Clustering Analysis.
A heat map is a graphical representation of data where each colored cell corresponds to an individual value contained in a matrix. Adjacent by HCA, the nine common peaks across ten SJZ samples were visualized by a heat map, and two clusters could be detected from Figure 3. Cluster 1 was composed of S1-S2, and cluster 2 consisted of S3-S10. In the heat map, the deeper color indicates the peak area of the SJZ samples of higher intensity. e results suggested that the content of chemical constituents contained in samples in cluster 1 was higher than that in cluster 2. e differences in the chemical ingredients could be related to the different geographic locations.
e results showed that the SJZ samples from different origins had similar chemical fingerprint, and HCA could initially separate the samples at the chemical level.

Analysis of the Spectrum-Effect
Relationship. CCA was used to study the spectrum-effect relationships between IC50 and area values of nine common peaks. As shown in Figure 4, the correlation coefficients demonstrated that eight peaks, namely, b, c, d, e, f, g, h, and i, were negatively correlated, while peak a was positively correlated. e value of IC50 which refers to the concentration to inhibit cell growth by 50% of the control level was negatively correlated with antitumor bioactivity of the SJZ sample. e results suggested that these compounds could inhibit the proliferation of PC9 cells. Eight correlated peaks, b, c, d, e, f, g, h, and i, have been identified previously as ginsenoside Rg1, Re, Rb1, Rc, Ro, and Rd, ammonium glycyrrhetate, and atractylenolide III, respectively. Ginsenoside Ro was the most correlated (R � − 0.862) to the biological effect, and the bioactivity of ginsenoside Rg1 was the second highest (R � − 0.576). erefore, the above results indicate that ginsenoside Ro and ginsenoside Rg1 are the major antiproliferative constituents.

Discussion
TCM decoction is an oral liquid preparation prepared by decocting several Chinese herbal medicines according to the theory of "Jun-Chen-Zuo-Shi" and appropriate dosage ratio. It has the characteristics of good absorption, quick onset, and flexible composition, which fully embodies the essence of syndrome differentiation and treatment of TCM [21,22]. erefore, it is especially important to control the quality of TCM decoction because it directly affects clinical efficacy. However, due to the multitarget and multicomponent characteristics of TCM decoction, it is difficult to control the quality of TCM decoction and it has become a hot topic in the field of decoction research. Recently, many studies have proved that the spectral-effect relationship should be an effective method to control the quality of TCMs because it can connect chromatographic fingerprints with biological activity to explore quality markers related to clinical efficacy [23]. So, to ensure the quality and therapeutic consistency of TCM decoction, this approach may be a promising strategy to control its quality. e experimental results presented here indicate that ginsenoside Ro and ginsenoside Rg1 may be the main active components for inhibiting the proliferation of PC9 cells.
Consistent with this work, it has been reported that ginsenosides Ro and Rg1 could inhibit various types of cancer cells. Ginsenosides Ro could suppress autophagy by inhibiting autophagosome-lysosome fusion to sensitize esophageal cancer cells to 5-Fu-induced cell death [24] and inhibit the metastatic dissemination of colon cancer cells HT29 by suppressing the expression of p-ERK1/2 in HT29 cells [25]. Additionally, ginsenosides Ro is one of the  bioactive constituents of ShenMai, which is an adjuvant therapy for cancer by inhibiting human organic aniontransporting polypeptides (OATP)1B [26]. Meanwhile, ginsenosides Rg1 could exert cytotoxic activity against human lung cancer cell lines A549, H1264, H1299, and Calu-6 [27] and suppress thyroid cancer proliferation and migration by enhancing the expression of Cx31 [28]. Moreover, ginsenosides Rg1 could sensitize hepatoblastoma cells to DNA-damaging agent, making ginsenosides Rg1 a promising chemotherapeutic agent [29], and it is also expected to become a preventive vaccine adjuvant for lymphoma by activating dendritic cells [30]. us, ginsenosides Rg1 and ginsenosides Ro may be used as new markers for quality control of SJZ treating lung cancer in the future.
Our findings suggested that spectrum-effect relationship should be a useful tool to associate the "quality marker" with clinical efficacy of TCM decoction, and it also provided a paradigm for the quality control of other anticancer TCM decoction. However, the two compounds cannot be applied as indicators of the overall quality control of SJZ because antiproliferative effect on PC9 cells is only one of its various clinical effects. Moreover, we mainly focused on compounds with a strong UV signal due to instrument limitations (PDA detector). Ginsenoside Rg3 played an important role in the prevention and treatment of cancer, but due to its low content, it could not be detected in this research [31,32]. erefore, further research is needed to determine whether ginsenosides Ro and Rg1 can be used as quality control markers for treating other diseases and whether there are other potential active components against lung cancer in SJZ. Next, to explore more active constituents or metabolites, mass spectrometry analysis will be considered for this research.

Data Availability
e data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest
e authors declare that they have no conflicts of interest.