Effects of Opened and Closed Spillway Operations of a Large Tropical Hydroelectric Dam on the Water Quality of the Downstream River

Water quality downstream of a hydroelectric dam is potentially affected by dam operations and other land uses in the river basin. Previous short-distance studies below the large Bakun Dam indicated poorer water quality during closed spillway. However, the extent of the impact is still unknown. Such knowledge is essential for mitigating the impact of the dam..us, the objectives of this study were to determine the water quality up to a distance of 210 km under two spillway operations, namely, closed and opened spillways, and also to determine the changes in water quality from the predam condition. Physicochemical parameters were measured at 15 stations along the Rajang River. Results of this preliminary study indicated that there were significant differences in eight out of nine water quality parameters between opened and closed spillway operations with opened spillway showing better water quality. During closed spillway, as we approached the dam, there was an increasing acidity and a decreasing oxygen content. Furthermore, as the water flows downstream, the unhealthy DO level (<5mg/L) extended up to 165 km and the linear model showed an increasing DO rate of 0.09mg/L per km. With opened spillway, DO decreased exponentially from 9.74mg/L towards the downstream direction to 7.67mg/L..e increasing turbidity and TSS in the downstream direction indicate contributions from erosion due to other land uses..e river is polluted with organics as indicated by COD of Class IV or V with sources from the dam and the activities in the river basin. Compared to the predam condition, the regulated river is less turbid but warmer and higher in ammonia. Closed spillway led to lower DO and acidic water. However, opened spillway water pH and DOwere similar to those in the predam condition. .us, it is recommended that DO be consistently high enough for the health of sensitive aquatic organisms downstream.


Introduction
Energy from hydroelectric dams is a beneficial alternative to fossil fuels as it is renewable.Abundance of rainfall in a tropical country such as Malaysia provides the water flow needed for hydropower generation.us, the largest dam in Malaysia, the Bakun Hydroelectric Dam, was constructed across the longest river, the Rajang River, providing an installed capacity of 2400 MW [1], which started operation in November 2011.Just like most rivers in the world, the Rajang River also functions as a means of transportation, source of food and water, and habitat to various floras and faunas.However, the building of dams has been reported to impact the agricultural land and wildlife habitats of the flooded area, and downstream from the dam, floodplain hydrology, movement of sediments, channel structure, ecology, and biodiversity have also been impacted [2][3][4].In addition, the structural design and operation of a dam determine the flow regime and affect the downstream river in various ways including the water quality.In the Bakun Dam Reservoir, water quality studies during and after the impoundment [1,5,6] showed the impact of damming the Rajang River where dissolved oxygen decreased as depth increased.When the hypolimnion water is released downstream through spillways and turbines, there is a potential impact on sensitive aquatic organisms.It has been reported that dams can affect the downstream river up to hundreds of kilometers [7].It is therefore important to minimize the impact of the damming of a river on the ecosystem downstream.For downstream of Bakun Dam, knowledge on water quality is scarce.A downstream study conducted up to 32 km distance showed that there was a large difference between water quality during opened spillway and closed spillway [8].Results of another study conducted at the Pelagus area, more than 150 km downstream from the dam, showed low dissolved oxygen when the spillway was closed [9].us, more studies are needed downstream to assess the water quality under different operation regimes and the extent of the impact in terms of distance which is essential for management purpose.
erefore, the present study focused on determining the physicochemical parameters of water quality along the Rajang River downstream of Bakun Dam under two different operation regimes, namely, opened and closed spillways, covering a distance of approximately 210 km.In addition, a comparison was made with the water quality of the Rajang River prior to the impoundment.

Materials and Methods
e study area was located at Rajang River, Sarawak, below Bakun Dam, from Long Baagu to Kapit Town.Fifteen stations along the river were selected for this study as shown in Figure 1.Table 1 shows the global positioning system (GPS) coordinates, location description, weather condition, distance of each station from the dam, and the time of sampling conducted at each station.e selection of first four stations was based on major villages: station 5 was based on the town area, stations 6 to 13 were based on the major tributaries of the river, station 14 was located 1 km below the rapid, and station 15 was located about 1 km from the coal loading jetty near the Kapit Town area.e distance along the river from Bakun Dam to the furthest station above Kapit Town was approximately 210 km. e sampling trips were conducted on 12 April and 6 September 2016.For each trip, all the stations were covered on the same day.On 12 April 2016, the spillway was opened to release water in addition to the discharge through the turbines, whereas on 6 September 2016, the spillway was closed.e spillway was built for the purpose of flood control.
e discharge of water during opened and closed spillways and the total volume of discharge are shown in Table 2.
At every station, the in situ parameters measured were the temperature, pH, dissolved oxygen (DO), turbidity, and transparency.e measurements of temperature, pH, dissolved oxygen, and turbidity were carried at in situ by using a multiparameter sonde instrument (YSI 6920 V2-2).e transparency of water was measured by using a Secchi disk with a measuring tape.Triplicates of water samples were taken at the subsurface of the river water by immersing a 1 L acid-washed polyethylene bottle that was rinsed with the river water about 2 to 3 times without leaving any air bubbles.ey were labelled and kept in a cooler box for ex situ analyses which included total suspended solids (TSS), chemical oxygen demand (COD), nitrite-nitrogen (NO 2 -N), nitrate-nitrogen (NO 3 -N), and ammonia-nitrogen (NH 3 -N).COD was analysed by using the closed reflux, titrimetric method; NO 2 -N was analysed by using the Hach 8507 Diazotization Method, NO 3 -N was analysed by using the Hach 8192 Cadmium Reduction Method, and NH 3 -N was analysed by using the preliminary distillation step followed by Nessler's method.e analyses of NO 2 -N and NO 3 -N were determined by using Hach methods [10], and the concentrations were determined using DR900 in the field.e TSS, BOD, COD, and NH 3 -N were analysed according to the methods outlined in Standard Methods [11].
e analysis of TSS was done by filtering at most 1 L of water through a 1.0 µm pore-sized glass filter paper (Sartorius), dried in an oven at 105 °C to constant weight.e final weight of the TSS was calculated by using the following equation: where W f � final weight of the dried filter paper with residue (mg), W i � initial weight of the dried filter paper (mg), and V � total volume of water filtered (L).
As for COD and NH 3 -N, after the river water was collected in triplicates in a 120 mL acid-washed polyethylene bottle, it was added with 1 mL of concentrated H 2 SO 4 and kept cool at 4 °C to preserve the water sample to be analysed in the laboratory.e analysis of COD was done according to the closed reflux method.To every 2.5 mL of the water sample in a culture tube, 1.5 mL of digestion solution and 3.5 mL of H 2 SO 4 were added.e tubes containing water samples and reagents were digested at 150 °C for at least two hours.ey were left to cool and transferred to a conical flask.A few drops of ferroin indicator solution were added to the conical flask.Every water sample in the conical flasks was titrated with 0.025 M FAS until the colour changed from bluish green to reddish brown.COD was calculated by using the following equation: where A � mL FAS used for the blank, B � mL FAS used for the sample, M � molarity of FAS (0.025 M), and 8000 � milliequivalent weight of oxygen × 1000 mL/L.e standard method NH 3 -N involved two steps which were the preliminary distillation step and Nessler method [11].Instead of using distilled water, ammonia-free water was used in the whole analysis to avoid external contamination of ammonia.e procedure started by taking 100 mL of water samples and adding 10 mL of borate buffer, and the pH was adjusted to 9.5.e water samples were distilled using a semiautomatic distillation unit (VELP Scientifica, UDK 139).
e distillate was added with 10 mL of boric acid, and the pH was adjusted to 7 by using 1 N NaOH.e distillate was then  Journal of Chemistry made up to 100 mL.After the preliminary step, the Nessler method was used.e samples were prepared by filling 25 mL of the sample into a mixing cylinder.e blank was prepared by filling ammonia-free water into the mixing cylinder.ree drops of the polyvinyl alcohol dispersing agent, 3 drops of mineral stabilizers, and 1.0 mL of the Nessler reagent were added to each mixing cylinder.e solution was mixed and stabilized for one minute.e sample absorbance was measured at 425 nm using a DR3900 spectrophotometer, and concentration was determined using a calibration curve.
NO 2 -N and NO 3 -N were analysed in the field according to Hach [9] using a DR900 colorimeter.NO 2 -N was analysed by taking 10 mL of water into a sample cell.e NitriVer 3 reagent powder pillow was added into the sample cell.e cell was swirled and left to react for 20 minutes before taking the final reading.
e blank was prepared by using the original sample water.NO 3 -N on the contrary was done using 2 reagents, namely, the NitriVer 3 reagent powder pillow and NitraVer 6 reagent powder pillow.
e water sample (15 mL) was taken and placed into a mixing cylinder.
en, the NitraVer 6 reagent powder pillow was added into the cell.e cell was shaken for 3 minutes before left to react for 2 minutes.en, 10 mL of the sample was taken for the mixing cylinder and poured into a sample cell.e NitriVer 3 reagent powder pillow was added and mixed thoroughly for 30 seconds.e sample was left to react for 15 minutes before obtaining the final reading.e blank was prepared using the original sample water.
Differences in water quality parameters at the 15 stations were tested using the one-way ANOVA.e paired t-test was also conducted to compare the means of the two spillway operations.Correlation analysis was conducted.All data analyses were done using the SPSS version 24 package.e linear model (Equation ( 3)) and exponential model (Equation ( 4)) were explored for their suitability to estimate the change in DO as a function of distance during closed and opened spillways: where C is the concentration of DO (mg/L), a and b are constants in the regression fit, and D is the distance from the dam (km).

Results and Discussion
3.1.In Situ Water Quality Parameters.Temperature is important for the ecological behaviour and distribution of aquatic organisms as temperature influenced their psychological processes such as food consumption, digestion, and immunity [12].
e temperature during opened spillway ranged from 26.88 °C to 27.80 °C where the highest mean was recorded at station 10 (123.4 km from Bakun Dam) and the lowest mean was recorded at station 5 (38.2 km from Bakun Dam) (Figure 2).e temperature during closed spillway ranged from 27.09 °C to 27.93 °C where the highest mean was recorded at station 15 (210 km from Bakun Dam) and the lowest mean was recorded at station 5 (38.2 km from Bakun Dam).During opened spillway, stations 1 to 5 showed lower temperatures than closed spillway due to the flow of colder water from the spillway of Bakun Dam as it was a rainy season.Statistical analysis also shows that, during opened spillway, the mean temperature of the whole stretch studied was significantly lower than that during closed spillway (p < 0.05) (Table 3).e fluctuation in temperature during each trip was due to the impact of the time of sampling in the day coupled with the weather condition, whereby temperature was observed to be higher in the afternoon than morning.
e energy transport process and heat fluxes influenced the temperature of the water surfaces [13].However, comparing stations studied at about the same time in the day gives an indication of the general trend of temperature as the water flows downstream along the Rajang River.For instance, during opened spillway, comparisons on the pairs of stations, 2 and 12, 4 and 14, and 5 and 15, gave an indication of an increasing trend of temperature as distance from the dam increased; similarly, during closed spillway, the pair of stations 5 and 11, though not as much increases, is significant (p < 0.05).
e pH during closed spillway ranged from 5.60 to 6.46, whereas during opened spillway, it ranged from 6.25 to 6.48 (Figure 3).Statistical analysis of the mean pH of the two spillway operations shows that, during closed spillway, pH was significantly lower than that during opened spillway (p < 0.05) (Table 3).In both occasions, as we approached the dam from downstream, pH decreased with the lowest pH observed at station 1 (4.3 km) which is the nearest station to the Bakun Dam.In addition, during closed spillway, the water pH was lower than that during opened spillway up to a distance of 178 km away from the dam after which the pH values were similar to those during opened spillway.pH during both trips falls in the range of 4.93-7.36near the water intake point in the reservoir [6].During opened spillway, at the beginning in station 3, as the water flows downstream, pH stayed relatively constant.e higher pH during opened spillway was similar to that reported in [8] when the water was studied up to a distance of 32 km from the dam.e pH values of stations 4 and 5 during closed spillway were significantly higher most likely due to increasing photosynthesis in response to nutrients from settlements along the river and the dilution of tributary river water.
is is supported by the significant correlation 4 Journal of Chemistry between pH and DO (r � 0.755, p < 0.05) as DO is produced during photosynthesis and carbon dioxide is consumed in the process.In both trips, station 5 showed a significantly higher pH value than station 4 due to the inflow from a big tributary (Belaga River).During closed spillway, there was more fluctuation in pH than during opened spillway, indicating higher susceptibility to the surrounding environmental influence such as diurnal variation in solar radiation which affected pH through algae photosynthesis and respiration and also inputs from settlement and tributaries.In terms of classification, during opened spillway, the pH values complied with Class II according to NWQS at all the stations (Table 4) [14].However, during closed spillway, the pH values at stations 1, 2, 3, and 6 dropped below 6.0 and thus were classified as Class III, while the other stations were classified as Class II.e pH value classified into Class III and below will negatively affect the sensitive fish species such as a deterioration in their appetite, growth capacity, and their tolerance to toxicity [15].Dissolved oxygen (DO) is an important parameter for the health of sensitive aquatic organisms.During opened spillway, the DO concentrations were high, ranging from 7.67 mg/L to 9.74 mg/L (Figure 4).e highest mean was recorded at station 1, the nearest to Bakun Dam, and the lowest mean was recorded was at station 15, the furthest from the dam.As we approached the dam, there was an increasing trend in DO.However, during closed spillway, the opposite was observed, whereby the DO values ranged from 3.50 mg/L to 6.02 mg/L with the highest mean recorded at station 15, the furthest from Bakun Dam, and the lowest mean recorded at station 1, the nearest to the dam.As we approached the dam from station 15, there was a general decreasing trend in DO with a few exceptions such as stations 2, 3, and 5. Comparing the values of DO, during opened spillway, all the stations showed higher DO than closed spillway and the difference between the means was significant (p < 0.05) (Table 3).When the spillway was opened, the highest DO value was observed at station 1 due to the rapid aeration from the flip bucket spillway design which contributed to the additional oxygen gas bubbles from the atmosphere entering the water [16].
e DO level during opened spillway started decreasing against distance due to the decrease in reaeration as distance increased and also mixing with lower DO water.In spite of that, the DO level still exceeded 7.0 mg/L which is ideal for the sensitive aquatic organisms.During closed spillway, the low DO at station 1 was due to the low DO content water from the reservoir flowing out through the turbines of the dam.According to the water intake structure, it was designed to withdraw from the top 10 m.However, the DO in the Bakun Reservoir, near the water intake, had been shown to drop sharply from about 4 m depth and by 6 m depth, and it was anoxic [6].DO during the closed spillway gradually increased along the downstream of the river as the water is aerated and diluted with higher DO river water and from the tributaries.Even then, the DO continued to be less than 5 mg/L up to about 165 km from Bakun Dam.Although the tropical aquatic organisms can withstand low DO levels, the ideal DO required for the sensitive aquatic organism's growth is at the minimum of 5 mg/L [17].Stations 2 and 14 were below rapids where higher reaeration occurred resulting in an increase in DO from station 1 to station 2 and from station 13 to station 14.From station 4 to station 5 and from station 12 to station 13, the increase was due to the inflow of higher DO water from Belaga River and Peraran   Besai River, respectively.Low DO downstream of a dam is also reported below Nam Ngum I Reservoir where along the 5 km stretch, monthly measured DO was mostly below 5 mg/L [18].e DO during opened spillway was classified as Class I, while during closed spillway, the DO was classified as Class II from station 13 to station 15 and Class III from station 1 to station 12 (Table 4).During closed spillway, the linear model used to estimate the change in DO during closed spillway shows that the rate of increase was only 0.009 mg/L per km from station 1 to station 15 inclusive of rapids reaeration and inflows from big tributaries along the way.As for opened spillway, the loss rate in DO (−0.010 mg/ L per km) estimated from data of stations 6 to 12 using the linear model was similar to that during closed spillway.However, from station 1 to station 12, that is, up to a distance of 154 km away from the dam, the exponential decay model fits the DO data better than the linear model with the coefficient of distance, b, of 9.648 and exponent, a, of −0.001 as shown in Figure 5. e turbidity during opened spillway ranged from 48.23 NTU to 90.26 NTU, and the highest mean recorded was at station 15, the furthest from the dam, while the lowest mean recorded was at station 1, the nearest to the dam (Figure 6).ere is a general trend of increasing turbidity at a rate of 0.15 NTU per km as the water flows downstream.During closed spillway, the turbidity values ranged from 47.97 NTU to 229.65 NTU with the highest mean recorded at station 15 and the lowest mean observed at station 2. Station 2 turbidity was the lowest due to the slower flow of water than the other stations which means less turbulence, and thus, deposition of solids took place.Similar to opened spillway, turbidity values during closed spillway showed a general increasing trend with the increasing distance from the dam.However, there were significantly higher turbidity values recorded at some stations such as stations 7, 8, 11, to 15 due to the rain which contributes to the high turbidity as the interaction of rainfall and the exposed soil causes erosions of sediments contributing to the addition of silts, clays, and suspended solids which increases the turbidity of water [11].
is is supported by the high correlation between turbidity and TSS (r � 0.857, p < 0.05).In this study, the turbidity of the river falls in the range between 50 mg/L and 250 mg/L.However, the turbidity measurements conducted in the past studies stated that the turbidity is less than 50 mg/L at the depth of 10 m to 20 m in the dam reservoir in  6 Journal of Chemistry 2015 [5,6].us, the spillway and turbine outflow of Bakun Dam is not the source of the high turbidity downstream.e means between the two trips for turbidity were significantly different from each other (p < 0.05) (Table 3).During opened spillway, the turbidity at all of the stations exceeded 50 mg/L except station 1.For closed spillway, all the stations also showed turbidity exceeding 50 mg/L except station 2. us, according to NWQS, all stations during the two trips exceeded Class II with the exception of station 1 during opened spillway and station 2 during closed spillway which are classified as Class II (Table 4).

Ex Situ Water Quality Parameters.
e total suspended solids (TSS) during opened spillway ranged from 17.0 mg/L to 90.2 mg/L, whereas higher values ranging from 26.0 mg/L to 130.0 mg/L were observed during closed spillway (Figure 7) with the means between two trips significantly different from each other (p < 0.05) (Table 3).e trends of TSS during both opened and closed spillways were similar to that of the turbidity (Figure 6).During both opened and closed spillways, increasing TSS were observed from station 3 onwards.is is due to the erosion from numerous cleared forest logging activities and oil palm plantation development activities.During closed spillway, TSS increased significantly from station 7 to station 8 and from station 12 to station 13 due to heavy rain during the sampling time which resulted in resuspended sediments.In addition, the sharp increase in TSS from station 12 to station 13 was also due to erosion from a large logging area with exposed soil.During opened spillway, there was a significant increase in turbidity from station 4 to station 6. is is due to the input from the large Belaga tributary where upstream there was active logging and oil palm plantation development activities resulting in soil exposure to rainfall events as it was the rainy season.e heavy rain generated the surface runoff which moved sediments into the river increasing the TSS as supported by a previous study in Baleh River which showed that there was a tenfold increase in TSS after the rain compared to that before the rain [19].A previous study at the Pelagus area also reported a high TSS concentration of 380 mg/L in the Rajang River observed after raining the night before sampling.Significantly higher TSS at station 15 were mainly due to the soil erosion from the exposed land due to logging activities [20] and the exposed soil at the coal mining loading bay.Station 15 is also below the coal mine loading bay where such an activity increases soil exposure and erosion.TSS during open spillway were mostly Class II except for stations 2 and 3, which were Class I, and stations 6, 8, 12, 14, and 15, which were classified as Class III (Table 4).During closed spillway, six stations (1, 2, 3, 4, 6, and 10) were classified as Class II, while nine stations (5, 7, 8, 9, 11, 12, 13, 14, and 15) were classified as Class III of NWQS.
Chemical oxygen demand (COD) measures the amount of oxygen required to oxidize the chemically oxidizable compounds.During opened spillway, the COD concentration ranged from 61.3 mg/L to 173.3 mg/L as shown in Figure 8. e highest mean recorded was at station 1 which is nearest to the dam and the lowest mean recorded was at station 10, 123.4 km away from the dam.ere was a significant decrease from station 1 to station 4 (p < 0.05) followed by no significant difference between stations 4 and 5 (p < 0.05).e decrease was countered by the input from the Belaga tributary and wastewater inflow from Belaga Town.During closed spillway, the concentration of COD ranging from 53.3 mg/L to 130.6 mg/L was lower than that during opened spillway at most of the stations.Overall, the COD during opened spillway was significantly higher than that during closed spillway (p < 0.05) (Table 3).e COD during closed spillway peaked at station 5, indicating organic waste inputs from the Belaga Town and Belaga tributary where there are human settlement, oil palm activities, and logging camps.Sources of organic substances are from both the Bakun Dam outflow and the development in the river basin itself.According to past research, the COD concentration in the Bakun Reservoir ranged from 6.7 mg/L to 118.7 mg/L from 0 to 30 m depth in 2013 [5], and BOD 5 exceeded Class II at 0-20 m depth [6], indicating oxygen demand due to organic materials in the reservoir.Organic materials are also Journal of Chemistry attributed to the domestic wastewater and runoff from the settlement near to Bakun Dam, logging camps, oil palms, and towns and villages along the main Rajang River and its tributaries as shown by other studies [6,9,21,22].COD of household wastewater from a residential area in Kuching has been reported to be 165 mg/L [23].ere is a significant correlation between COD and NO 3 -N (r � 0.638, p < 0.05) and COD and NO 2 -N (r � 0.390, p < 0.05), indicating organic waste.e closed spillway turbine outflow COD of 93.3 mg/L in the present study is close to that in the previous study, but the opened spillway COD is higher than the value reported (80 mg/L) [8].In comparison with the range of COD (43.8 mg/L to 61.4 mg/L) in the past study in Sembrong Dam, Johor, in 2015 [24], the range of COD concentrations in the present study in Rajang River is observed to be higher for both opened and closed spillways.During opened spillway, all stations except stations 10 to 13 have COD concentration higher than 100 mg/L, and they fall into Class V of NWQS (Table 4).Stations 10 to 13 on the contrary were classified as Class IV.During closed spillway, all stations are in Class IV (50-100 mg/L) except six stations (3, 4, 5, 11, 12, and 15) with concentrations exceeding 100 mg/L, and thus, they are classified as Class V of NWQS.Ammonia-nitrogen (NH 3 -N) is one of the three main forms of nitrogen which indicates the status of organic nutrients enrichment and the condition of the water bodies [3,25].During closed spillway, the NH 3 -N values ranged from 0.12 mg/L to 0.37 mg/L.e highest mean was recorded at stations 5 and 6, while the lowest mean was recorded at station 3. e NH 3 -N during opened spillway ranged from 0.06 mg/L to 0.49 mg/L as shown in Figure 9. e highest mean recorded was at station 5 (38.2 km from Bakun Dam), and the lowest mean recorded was at station 9 (104.3km from Bakun Dam).During the opened spillway, the NH 3 -N concentration was significantly higher in stations 1, 5, and 15. e input from spillway contributes to the high concentration of NH 3 -N which was also observed in a previous study on the 32 km stretch [8].e past research conducted in the Bakun Dam showed that the area nearest to the water intake has a range of 0.016 to 0.35 mg/L for NH 3 -N [5].Station 5 was significantly higher than station 4 during both trips due to the waste input from Belaga Town and Belaga River through the runoff and waste from commercial and residential areas.Main nitrogen-ammonia sources along the river are residential areas, sewage systems, and commercial areas [26].Station 15 on the contrary has significantly higher ammonia-nitrogen due to the waste from active coal mining activities.e overall means for NH 3 -N between two trips were not significantly different from each other (p < 0.05) (Table 3).e NH 3 -N during opened spillway was classified as Class II for all stations except for stations 1, 5, and 15 which were classified as Class III (Table 4).During closed spillway, all the stations were classified as Class II, except for stations 5, 6, and 15 which were classified as Class III.Stations 5 and 15 consistently fall into Class III.
Figure 10 shows the concentrations of nitrate-nitrogen (NO 3 -N) during opened and closed spillways.
e concentration of NO 3 -N during opened ranged from 0.04 mg/L to 0.13 mg/L.e highest mean recorded was at station 2, and the lowest mean was recorded at stations 10, 13, and 14.When the spillway was opened, stations 1 to 4 NO 3 -N was observed to be not significantly different from one another and the highest among the stations due to the formation of NO 3 -N from the oxidation of ammonia in the presence of high DO (Figure 4), which is supported by the significant and high correlation between NO 3 -N and DO (r � 0.867, p < 0.05), with the corresponding decrease in NH 3 -N (Figure 9).During closed spillway, the NO 3 -N values, ranging from 0.00 mg/L to 0.04 mg/L, were lower at all the stations.e low NO 3 -N at stations 1 and 2 concurs with previous observation at the station near the water intake in the Bakun Reservoir, where the values less than 0.004 mg/L from 0 to 10 m depth [27], 0.008-0.020from 0 to 10 m depth [6], and 0.023-0.027from 0 to 15 m depth were observed [5].e highest mean was recorded at stations 5, 10, and 15, while the lowest mean was recorded at station 9. e higher valued stations were most likely contributed by domestic waste.e means for NO 3 -N between two trips were significantly different from each other (p < 0.05) (Table 3).e concentration of NO 3 -N in all stations for the two trips complied with Class II of NWQS for Malaysia (Table 4).
e nitrite-nitrogen (NO 2 -N) is less dominant than NO 3 -N in river as NO 2 -N is the intermediate compound when ammonia is oxidized to NO 3 -N [28].NO 2 -N during opened spillway ranged from 0.002 mg/L to 0.008 mg/L as shown in Figure 11.e highest mean recorded was at station 15, and the lowest mean recorded was at station 7. Spikes were observed at stations 5 and 8. From station 11, the reading began to increase until station 15.During closed spillway, the NO 2 -N values at almost all the stations were lower (0.000-0.005 mg/L).e highest mean was recorded at station 15, while the lowest mean was recorded at station 13.Similar to NO 3 -N, the Bakun Dam in past research has concentration of NO 2 -N more than 0.004 mg/L [5].During closed spillway, only station 15 was observed to have significantly higher NO 2 -N concentration more than 0.004 mg/L. is is due to the presence of coal mine in the area.Excavation and mining of coals from coal mines cause possible leaching of nitrogen elements into the river [29].In addition, DO increased as distance from the dam increased providing oxygen needed for the oxidation of ammonia to nitrite, as supported by the significant correlation between NO 2 -N and DO (r � 0.403, p < 0.05), and subsequently to nitrate.us, this increases the concentration of NO 2 -N in the river particularly at station 15. e means for NO 2 -N between two trips were significantly different from each other (p < 0.05) (Table 3).e concentration of NO 2 -N at all stations for the two trips complied with Class II of NWQS for Malaysia (Table 4).
A comparison of water quality of Rajang River in the present study based on the dam operations, namely, opened and closed spillways, with that before building the Bakun Dam is shown in Table 5. e present study shows that when the spillway was closed, there was a lowering of pH, DO, and TSS and an increase of temperature and NH 3 -N.When the spillway was opened, there was also a lowering of TSS with pH and DO being comparable with that before damming. is indicates that damming has the advantage of lowering TSS in the water.In addition, the impact on the aquatic life downstream could be mitigated by opening the spillway which will raise the DO and pH.

Conclusions
is study shows that there is a significant effect of the operations of Bakun Dam spillway on the water quality of the 210 km stretch of the downstream river towards Kapit Town.With closed spillway, as we approach the dam, there was a general decreasing trend in pH and DO, indicating that the water was increasing in acidity with less oxygen content and it became much more acidic and much less in DO than opened spillway water.e impact of unhealthy DO stretched to a distance of about 165 km from the dam.As the water flows downstream from the dam, it is more susceptible to other influences such as input from settlements, tributaries, and diurnal changes in solar radiation when the spillway was closed than when opened except for parameters such as turbidity and total suspended solids from large tributaries where there is erosion during opened spillway which coincided with the rainy season.e river suffers organic pollution as indicated by COD which falls either into Class IV or into V with opened spillway showing worse condition than closed spillway.e high ammonia in opened spillway water and from the Belaga area was quickly oxidized to nitrate due to the abundance of oxygen.Comparisons of spillway operations with natural river prior to the dam construction show that, during closed spillway, there was a decrease in pH and DO, and under both spillway operations, there was a decrease in TSS but an increase in temperature and ammonia.When the spillway was opened, pH and dissolved oxygen are comparable with the natural river prior to dam construction.As such, it is recommended that, during closed spillway, DO should be increased for the health of sensitive aquatic organisms along the river downstream.

Data Availability
e raw data and processed data used to support the findings of this study are available from the corresponding author upon request.

Figure 1 :
Figure 1: e location of the 15 sampling stations along the Rajang River, below Bakun Dam to Kapit in Sarawak, Malaysia.

Figure 2 :
Figure 2: Temperature at the 15 stations downstream of Bakun Hydroelectric Dam during opened spillway (o) and closed spillway (•).e same letter indicates no significant difference (p > 0.05).

Figure 3 :
Figure 3: pH at the 15 stations downstream of Bakun Hydroelectric Dam during opened spillway (o) and closed spillway (•).e same letter indicates no significant difference (p > 0.05).

Figure 7 :Figure 6 :
Figure 7: Total suspended solids at the 15 stations downstream of Bakun Hydroelectric Dam during opened spillway (o) and closed spillway (•).e same letter indicates no significant difference (p > 0.05).

Figure 8 :
Figure 8: Chemical oxygen demand at the 15 stations downstream of Bakun Hydroelectric Dam during opened spillway (o) and closed spillway (•).e same letter indicates no significant difference (p > 0.05).

Table 1 :
Sampling stations with distance from Bakun Dam, GPS coordinates, location description, time, and weather conditions during opened and closed spillways.

Table 2 :
Discharge from turbines and spillways during opened and closed spillways on the two sampling dates.

Table 3 :
Mean and standard deviation of the water quality parameters in the Rajang River during opened and closed spillways.
bMeans in the same row with the same letters are not significantly different (p > 0.05).

Table 4 :
[14]sification of water quality parameters for all stations during opened and closed spillways according to NWQS[14].

Table 5 :
[30]r quality parameters of the present study compared with the values before building the Bakun Dam[30].