Investigation of Acid-Base Indicator Property of Plumbagin from Plumbago zeylanica Linn

There has been an increasing interest in the search for colour indicators of natural origin for titrimetric analysis. This is due to some challenges associated with the currently used synthetic ones. This study evaluates and validates the acid-base indicator property of plumbagin isolated from Plumbago zeylanica Linn. Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) was isolated from the roots of Plumbago zeylanica Linn using silica gel chromatography and characterized using spectroscopic methods in comparison with those reported in the literature. Its acid-base indicator property was evaluated alongside phenolphthalein and methyl orange, after it was found to exhibit a sharp change in colour at various pH ranges. The plumbagin indicator was successfully used to assay ibuprofen powder and tablets (400 mg) using the British Pharmacopoeia (2013) method. Data obtained were analyzed statistically by Student's t-test and one-way ANOVA in GraphPad Prism (version 5.01, 2010). Analysis of the use of the plumbagin indicator in acid-base titrations between strong acids and strong bases and between weak acids and strong bases has been evaluated and validated according to the ICH guidelines. Plumbagin use in ibuprofen powder and tablets has also been verified. Plumbagin has been validated for use as an indicator suitable for different acid-base titrations and the analysis of ibuprofen.


Introduction
Titrimetric analysis remains an important analytical technique in pharmaceutical analysis. It enables keen monitoring of the acid and base contents of raw materials, reaction mixtures, and resulting finished products in the industrial setting [1]. is method has several advantages including time and labour-saving, high precision, and determination of the purity of compounds in the absence of reference standards [1,2]. In the past, this method has also been used to analyse and estimate the degraded products of drugs [2].
In acid-base titrations, acids are titrated with bases, and vice versa. ey are also referred to as neutralization reactions, with the equivalent point detected either by use of colour indicators or potentiometrically with a glass electrode [3,4]. ey employ colour indicators to detect endpoints of acid-base neutralization reactions. Colour indicators show a sharp change in colour, in response to pH change in an acid-base titration reaction. Most indicators are either weak organic acids or basic dyes that accept or donate electrons [1,5]. Acid-base indicators of natural origin have proven to be better alternatives in titrimetric analysis, as they are readily available, easy to extract, cheaper, less toxic, and environmentally friendly, unlike synthetic colour indicators which are associated with high cost and toxicity to the environment and users [4,6,7]. Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) is a natural organic dye found in plants of the families Plumbaginaceae, Droseraceae, Ebenaceae, Iridaceae, Ancistrocladaceae, Drosophyllaceae, and Nepenthaceae [8,9].
Al-Nuri et al. [10] suggested that plumbagin may have acid-base indicator property in a study to spectrophotometrically quantify the compound in different parts of Plumbago europaea L. [10]. Hence, plumbagin isolated from Plumbago zeylanica Linn roots was evaluated for acid-base indicator property in this study. We hereby report our findings which reveal that plumbagin shows a prominent colour indicator property in acid-base titrations.

Collection and Authentication of Plant Materials.
Roots of Plumbago zeylanica Linn were collected in September 2018 from the Physic Garden of the Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology (KNUST). It was authenticated at the Department of Pharmacognosy, KNUST, with voucher number 003/10/07.

Isolation of Plumbagin.
e powdered roots (2.4 kg) were cold macerated with ethyl acetate (10 L) for 5 days. e extract obtained on decantation was filtered by gravity using Whatman ™ cellulose filter papers (1442-125, Grade 42, 2.5 μ, 12.5 cm), and the filtrate was concentrated with a rotary evaporator at a temperature of 40°C and controlled vacuum pressure, to a 2.5% w/w dry dark-brown solid mass. e resulting slurry from the dry dark-brown solid mass for column chromatography was prepared by dissolving the dried extract in methanol, while adding small amount of silica. e mixture obtained was continuously stirred to allow for evaporation of the methanol to obtain a yellowishorange solid product. e borosilicate chromatography column (90 cm × 6 cm) (Merck, Germany) with a fitted disc support was dried packed with silica (70 : 230 mesh size; stationary-phase material). Crude plumbagin (2.7 g) was eluted with a mobile-phase composition of ethyl acetate (15% v/v) in petroleum ether (85% v/v) followed by recrystallization from hot petroleum ether to obtain orange crystals (1.5 g). For the recrystallisation, crude plumbagin (2.7 g) was transferred into a conical flask (50 mL) followed by addition of petroleum ether. e mixture was heated to 60°C which dissolved the crude product completely. e resulting solution was allowed to stand at room temperature (28°C) which afforded gradual formation of needle-like crystals of plumbagin. e crystals were filtered by gravity using Whatman ™ cellulose filter papers (1442-125, Grade 42, 2.5 μ, 12.5 cm) and allowed to dry at room temperature (28°C). e pure isolate's identity was established with melting point determination, 1D NMR (proton and carbon-13), and 2D NMR (COSY, HMBC, HSQC, DEPT-135) spectroscopy with the support of infrared (IR) and ultraviolet-visible (UV-Vis) spectroscopy techniques.

Screening of Plumbagin for Indicator Property.
Plumbagin (0.05 g) was rendered completely soluble in methanol (50 mL) and diluted to the 100 mL mark with the same solvent in a volumetric flask to obtain a concentration of 0.05% w/v. Solutions of different pH (1-13) were prepared serially using 0.1 M·HCl, 0.1 M·NaOH, and distilled water (for pH 7) in test tubes. Plumbagin (5 drops) was added to the prepared solutions at room temperature, and observations were made for colour changes. e actual pH of the solutions where sharp colour changes were observed was determined with a calibrated pH meter as the working pH range of the plumbagin indicator.
2.6. Titrimetric Analysis (Colour Indicator). Standard 0.1 M·HCl (10 mL) was titrated with standard 0.1 M·NaOH (strong acid/strong base) at room temperature using methyl orange as the standard indicator and the plumbagin solution under investigation. e endpoints from use of both indicators were determined visually. Replicate determinations were made, and the data obtained were statistically analyzed by one-way ANOVA in GraphPad Prism (Version 5.01, 2010).
Similarly, 0.1 M·CH 3 COOH solution (10 mL) was titrated with 0.1 M·NaOH (weak acid/strong base) using phenolphthalein as the standard indicator and the plumbagin solution under investigation. e endpoints were determined visually by colour changes of the indicators used. Replicate titrations were carried out, and the results were statistically analyzed by one-way ANOVA in GraphPad Prism (Version 5.01, 2010).

Assay of Ibuprofen BP Powder and Tablets.
e British Pharmacopoeia method was used to assay ibuprofen BP powder (0.4500 g) and tablets (equivalent weight of powder containing 0.4500 g) [12]. e prepared ibuprofen solutions were titrated with standard 0.1 M·NaOH (titrant). Replicate analysis was carried out, and the obtained results were analyzed statistically by one-way ANOVA in GraphPad Prism (Version 5.01, 2010).

Validation of Indicator Property of Plumbagin.
e use of the plumbagin indicator in titrations involving HCl/ NaOH (strong acid/strong base) and CH 3 COOH/NaOH (weak acid/strong base) and in the assay of ibuprofen BP powder and tablets (400 mg) has been validated according to the ICH guidelines. e parameters evaluated were accuracy, precision (repeatability, interday precision, and intraday precision), robustness, and specificity.

Characterization of the Isolate (Plumbagin).
Pure plumbagin (1.5 g) was obtained as orange needle-like crystals with a melting point of 78-80°C. e retardation factor (R f ) was obtained on a silica gel-coated TLC plate developed with the mobile phase of petroleum ether-ethyl acetate (70% v/v : 30% v/v) as 0.82. e UV-visible spectrum showed two absorption maxima at 265 nm and 420 nm, identical to that reported by Annan et al. [11]. e IR spectrum revealed presence of a broad band at 3293 cm − 1 indicative of an OH group. Strong peaks observed at 1161.60 cm − 1 and 1639.66 cm − 1 are indicative of free and hydrogen-bonded carbonyls, respectively. Vibrations at 1605.48 cm − 1 are due to aromatic C�C. A weak absorption at 3038.4 cm − 1 indicates -C-H stretches of sp 2 hybridized carbons of an aromatic system. Vibration occurring at 1227.90 cm − 1 is indicative of a phenolic CO group.
In the 1 H NMR (CDCl 3 ) spectrum, a doublet peak occurred at δ 2.18 (3H, d) and is due to the three protons of the methyl group present. A singlet peak at δ 6.79 is assigned to the quinoid ring proton at position C-3. Multiplet signals observed at δ 7.24-7.61 (3H, m) resulted from the three aromatic protons at positions C-6, C-7, and C-8, respectively. A singlet peak occurring at δ 11.94 (1H, s) is attributed to the intramolecularly hydrogen-bonded phenolic proton. e 13 C NMR (CDCl 3 ) spectrum revealed a total of 11 carbons in the compound. Two peaks occurring at δ 184.73 and δ 190.24 are indicative of a free carbonyl (C-1) and hydrogen-bonded carbonyl (C-4), respectively.

Acid-Base Titrimetry.
Plumbagin indicator solution of 0.05% w/v concentration was prepared in methanol ( Figure 2(a)). Colour changes observed in different pH solutions resulted in identification of plumbagin as an indicator. Drops of methanolic solution of plumbagin gave a yellow colouration in the acidic medium and pink colour in the basic or alkaline medium. e phenolic OH group attached to the naphthoquinone nucleus is protonated in the acidic medium, leading to the reduction of electron cloud density, which results in absorption at wavelengths of 248 nm (λ max ) and 420 nm (hypsochromic shift and hypochromic effect) (Figure 2(b)). Alternatively, the phenolic OH is ionized in the alkaline medium (1), resulting in the increase in electron cloud density and absorption at 275 nm (λ max ) and 520 nm (bathochromic shift and hyperchromic effect) (Figure 2(c)). e working pH range of the plumbagin indicator was determined to be 8.02-10.07.
Statistical analysis using one-way ANOVA showed no significant difference in the results obtained from use of methyl orange and plumbagin in titration of HCl with NaOH (p � 0.7730). Similarly, no significant difference is seen with the results obtained from use of phenolphthalein and plumbagin in titrations of CH 3 COOH with NaOH (p � 0.0896) ( Table 1).
Indicator errors of the two standard indicators as well as plumbagin were determined under similar conditions. Statistical analysis by Student's t-test did not show a significant difference between methyl orange and plumbagin. No significant difference was observed with indicator errors of phenolphthalein and plumbagin as well.

Validation of Use of Plumbagin
Indicator. e accuracy of an analytical method is the closeness of the test results obtained to the mean or the theoretical true value [1]. e accuracy of the plumbagin indicator was determined in titrations involving 0.1 M·HCl/0.1 M·NaOH and 0.1 M·CH 3 COOH/0.1 M·NaOH, by comparing endpoint results obtained with those from use of methyl orange and phenolphthalein, respectively. Statistical analysis of endpoints using a two-tailed Student's t-test (in GraphPad Prism) showed no significant difference in endpoint values obtained with the use of plumbagin and methyl orange indicators (p � 0.7730) (Figure 3(a)). No significant difference was also observed with the use of plumbagin and phenolphthalein indicators (p � 0.0896) (Figure 3(b)). e indicator errors of   plumbagin and the standard indicators were also compared statistically using Student's t-test at a confidence interval of 95% in further evaluation of accuracy. e results showed no significant difference in indicator error compared to those of methyl orange (p � 0.9674) (Figure 3(c)) and phenolphthalein (p � 0.0896) (Figure 3(d)). Accuracy was further evaluated with percentage recovery obtained from the assay of ibuprofen powder using plumbagin as an indicator. e percentage recovery ranged between 98.80% and 100.81%, with a mean of 99.94%. e obtained results fall within the acceptance criteria of 98.0%-102.0% (Table 2).
Precision refers to the closeness of agreement of results under defined conditions. It is expressed as standard deviation (SD) or relative standard deviation (RSD) [13]. Evaluation of precision by repeatability was achieved using plumbagin (0.10%) in titrations between 0.1 M·HCl and 0.1 M·NaOH and between 0.1 M·CH 3 COOH and 0.1 M·NaOH. e RSD for ten replicate determinations was obtained to be 0.48% and 0.61%, respectively, which agrees with the acceptance criteria ≤2.0% (Table 3). e RSD obtained for percentage purity and content of ibuprofen powder and tablets (400 mg) was 0.41% and 0.68%, respectively, which also agrees with the acceptance criterion.
Evaluation of precision by intermediate precision was achieved through statistical analysis of results obtained by the same analyst on different experimental days (interday   precision) and results obtained by the same analyst on different experimental times (intraday precision). e RSD obtained for both interday and intraday precision results was found to comply with the acceptance criteria ≤2.0%. Statistical analysis by one-way ANOVA showed no significant differences (p ≥ 0.05) ( Tables 4 and 5). e robustness of an analytical method refers to the degree of reproducibility of test results obtained from same samples under varied or altered conditions [3].
is was evaluated using 50%, 100%, and 150% plumbagin, representing 0.05%, 0.10%, and 0.15%, respectively, with different drops of a particular concentration of plumbagin, and by different analysts on the same experimental day. e RSD of the results obtained in all the analysis complied with acceptance criteria ≤2.0%. Statistical analysis of results obtained by one-way ANOVA failed to produce significantly different results on alteration of test conditions (p ≥ 0.05) ( Tables 6-8).
Specificity is the ability to unequivocally assess an analyte in the presence of expected components [14]. is was achieved through the establishment of presence of plumbagin in the indicator solution and attribution of titration endpoints to plumbagin, and not the solvent used to prepare the indicator solution (methanol). Five drops of plumbagin and methyl orange indicators were added to volumes (10 mL ± 0.1) of 0.1 M·NaOH, 0.1 M·HCl, and 0.1 M·CH 3 COOH in separate conical flasks. e solutions were titrated with 0.1 M·HCl and 0.1 M·NaOH, respectively, and colour changes were observed. Methanol did not affect colour changes in the analyte solutions, and as such, endpoints of the reactions between the titrants and analytes could not be monitored visually (Table 9). Colour changes therefore observed in the titrimetric analysis were attributed solely to the plumbagin indicator.
Stability of a colour indicator solution enables the prediction of the period within which the indicator is fit for its intended purpose or use [1]. e stability of plumbagin was determined with freshly prepared 0.05% indicator solution in titration of 0.1 M·HCl/0.1 M·NaOH over a 3-month duration with replicate determinations on weekly basis. e RSDs for all the determinations complied with the acceptance criteria ≤2.0% (Figure 4).

Conclusion
e acid-base indicator property of plumbagin has been evaluated and validated in accordance with the ICH guidelines. Plumbagin which was easily extracted from its   International Journal of Analytical Chemistry    source was found to play a dual role as an alternative indicator to methyl orange and phenolphthalein in titrations involving strong acids/strong bases and weak acids/strong bases. Hence, it can be used as an indicator in analytical laboratories and could be a suitable substitute for methyl orange and phenolphthalein for such titrations hitherto mentioned in this study.
Data Availability e data are available from the Pharmaceutical Chemistry Laboratory, Faculty of Pharmacy, KNUST, Ghana. However, the data used to support the findings of this research are within this article.

Conflicts of Interest
e authors declare that there are no conflicts of interest.  Week Endpoint Figure 4: Endpoints obtained from a three-month study of use of freshly prepared 0.05% plumbagin solution.