Determination of the Lipophilicity of Ibuprofen , Naproxen , Ketoprofen , and Flurbiprofen with Thin-Layer Chromatography

*e lipophilicity is an important parameter that influences the activity of the drugs in the human body. *e reversed phase high performance thin layer chromatography was applied to determine the LogP values of ibuprofen, ketoprofen, naproxen, and flurbiprofen. *e stationary phase used in the study was silica-gel coated plates. *e mobile phase was the mixture of acetonitrile and water in different proportions. *e content of acetonitrile varied in 5% increments from 50% to 80%. *e Rm0 values were determined for the compounds with a known LogP and for the analyzed substances (ibuprofen, naproxen, ketoprofen, and flurbiprofen). *e LogP values were calculated for the analyzed compounds using the regression curve Rm0 � f(Log P) parameters for the compounds with the known lipophilicity. Flurbiprofen is characterized by the highest LogP value: 3.82. *e lowest one is noted for ketoprofen: 2.66.*e determined LogP values of tested compounds were similar to the values calculated by the software.


Introduction
Lipophilicity is defined as the affinity of a molecule or moiety to a lipophilic environment.It is an important parameter described by the partition coefficient (P).It describes the partition of the compound between aquatic and nonpolar solvent.It is commonly assessed by the distribution in a liquid-liquid system (with the shake-flask method) or liquidsolid (using chromatographic techniques).Lipophilicity is a complex effect of molecular interactions both between solute and solvent as well as interactions between solvent molecules in each phase.e substance, when dissolved in the solvent, disrupts its structure, which causes the breakdown of intermolecular bonds.e substance must create a free space in a given volume of solvent.It requires a certain amount of energy and results in a clear correlation of the lipophilicity with the volume or surface of the dissolved molecule [1].
Lipophilicity affects the biological activity of a drug because it plays a significant role in drug interactions with the receptor, the pharmacokinetics of the molecule, its toxic action, and in the pharmaceutical aspect-the solubility of the substance [2].
e chemical substance undergoes a series of biophysical and biochemical transformations in a human body.
ey can be divided into three phases: pharmaceutical (drug form and its release), pharmacokinetic (drug transport), and pharmacodynamic phase (interaction with the receptor).e lipophilicity affects the pharmacokinetic processes in living organisms especially the distribution process.All substances that penetrate into the body's cell encounter lipophilic barriers (cell membranes).Only molecules with appropriate affinity for lipophilic biological membranes will pass through them.It results in the distribution of the drug in the human body, and the substance will reach the receptors.is feature is highly correlated with passive transport across cell membranes.
Various extraction systems can be used to determine lipophilicity.e n-octanol/water system is often used to determine the partition coefficient which is usually denoted as the logarithm of P (Log P). e n-octanol/water system is used in a classic shake-flask method [3].e determination of the Log P in the n-octanol/water system is widely used in chemistry, medicine, pharmacology, and toxicology to describe the transport through biological membranes, solubility, toxicity, and absorption of substances [4].However, it has some disadvantages-it is time-consuming and requires relatively more amount of the reagents when compared with chromatographic methods.Chromatographic division of the compounds with reversed-phase thin-layer chromatography (RP-TLC) between the nonpolar stationary phase and the aqueous mobile phase is similar to the division in the biological system.e stationary phase RP-18 (octadecylsilanized silica gel) is stable and resistant to many eluents.It is the most common stationary phase for RP-TLC and RP-HPLC.Its surface has anisotropic properties, similar to biological membranes, which cannot be attributed to the octanol phase in extraction methods.Long hydrocarbon chains on the surface of the adsorbent, forming a hydrophobic film, allow the analyte's molecule to penetrate to various depths, which more accurately corresponds to interactions with the body membranes.For this reason, reverse-phase chromatography should better correspond to biological lipophilicity than the static and isotropic extraction system [1].
ey are also applied topically in the treatment of traumatic, overload, or inflammatory changes in periarticular tissues and muscles as well as for the treatment of inflammations in the oropharyngeal cavity or the dental pain.Due to the analgesic activity, the investigated drugs are used in the treatment of muscular pains, after tooth extraction, after surgery, in neuralgia, in root syndromes, in discopathy, and also in migraine [5][6][7].
e concentration of the investigated solutions was 1%. e analyzed substances were dissolved in methanol.10 µL was transferred onto the plate.e chromatograms were developed in the horizontal chamber and visualized with the UV254 lamp.

2.2.
e Calculation of the Data.Using the following equation, the R f values were calculated: where x is the distance from baseline traveled by solute and y is the distance from baseline traveled by the solvent.e R f were used to calculate the parameter according to Bate-Smith and Westall equation [8,9]: e R m values were extrapolated to pure water (R m0 ) by the equation for the following linear function R m � f(c) [10]: where a is the slope of the curve which indicates the rate at which the solubility of the compound increases in the mobile phase [8], C is the concentration of the acetonitrile, R m0 is the R m of the compound when the concentration of ACN is zero (extrapolated value).e equations are listed in Table 1.
e precision was within the range 2.92-8.76%and 3.65-8.42for the compounds with known lipophilicity and the analyzed ones.e method was linear (|r| > 0.989).
e R m0 and log P values for the compounds with the known lipophilicity are listed in Table 2.
e calculated values for the inflammatory drugs are listed in Table 3.

Results and Discussion
All the equations listed in Table 1 have a negative slope.It implies that with the increase of the organic modifier concentration in the mobile phase, the R m value for less lipophilic compounds decreases more slowly than for more lipophilic derivatives.is means that lipophilic compounds are more sensitive to changing the polarity of the mobile phase.
e negative value of the slope in TLC equation suggests an increase in the migration of substances per unit of growth in the content of the organic solvent.e highest values of the slope characterize the compounds that are most susceptible for the change of the content of organic solvent in the mobile phase.e highest values are observed for flurbiprofen and ibuprofen, whereas the lowest values were observed for isatin (Table 1).
Lipophilicity is a valuable parameter of the drug which affects its activity in the human body.e Log P value of the compound indicates the permeability of the drugs to reach the target tissue in the body.All the investigated compounds are lipophilic because the Log P > 0 (or P > 1) (Table 3).It implies that they prefer the organic phase, and it reflects in their physical properties-they are practically insoluble in water.According to PubChem database, the lowest solubility in water is reported for flurbiprofen (8 mg/l) for which the highest Log P TLC value is reported in our study (Table 3).Ibuprofen and naproxen have similar solubility values which are 21.5 mg/l and 15.9 mg/l, respectively.e highest solubility was noted for ketoprofen (51 mg/l), and the least Log P TLC value is reported in our study.All the solubility values are reported for room temperature [13].e most lipophilic compounds are flurbiprofen and ibuprofen.Lower Log P values of ketoprofen and naproxen result from the presence of an additional oxygen atom, which is an acceptor of hydrogen bonds.It lowers the lipophilicity of these compounds compared to ibuprofen and flurbiprofen, which are free of additional oxygen.Ibuprofen is more lipophilic because of the presence of 2-methylpropyl moiety.Flurbiprofen has the highest Log P TLC value due to the presence of a fluorine atom.Flurbiprofen is more lipophilic than ketoprofen because it possesses the fluorine atom and the phenyl rings which are not bonded together by an oxygen atom (Figure 1).Li et al. [14] determined the lipophilicity of flurbiprofen in n-octanol/water system.Its Log K oct value was 4.24.Pyka et al. reported the following Log P values for ketoprofen, naproxen, ibuprofen, and flurbiprofen: 3.12, 3.18, 3.97, and 4.16, respectively [12].ey were determined also for the n-octanol/water technique.e Log P TLC values determined in our study are lower (Table 3).However, the strong linear and statistically significant correlation between these values is observed and is shown in Figure 2. e TLC technique is based on the interaction with the stationary phase (C-18).e hydrogen bonds play a significant role in the dissolution process.Substances which molecules can form hydrogen bonds (in this case with n-octanol) will be preferred in the partitioning process.An important conclusion can be drawn that the experimentally determined lipophilicity parameters depend on the applied system [1].
Pyka et al. [12] reported also the Log P values for flurbiprofen, ibuprofen, naproxen, and ketoprofen calculated with the various numeric methods such as A Log Ps, IA Log P, C Log P, Log P Kowwin , x Log P, and MI Log P. ese techniques take into consideration the impact of the atoms or group of atoms on the lipophilicity.IA Log P and Log P Kowwin are based on the molecular structures with measured lipophilicity.Having compared the values obtained in our study (Log P TLC ) with the computational Log P values reported in the study of Pyka et al. [12], the strong linear correlation is observed for A Log Ps, C Log P, and Log P Kowwin .e correlation coefficients (R 2 ) are 0.9427, 0.9614, and 0.9970 for Log P Kowwin, C Log P, and A Log Ps, respectively.e closest theoretical Log P values to the values Log P TLC determined in our study are observed for C Log P (Table 4).C Log P is the technique that estimates the interactions of the new fragments.It is more accurate than statistical methods because it is based on the established chemical interactions [12].e linear correlation is also observed for x Log P (R 2 0.8354).e weaker correlation is observed for the IA Log P (R 2 0.6451).
e lowest one is noted for mi Log P (R 2 0.4859).e values of Log P determined with the n-octanol/water system are similar to computational ones.It might be caused by the fact that some of the computational techniques are useful for the prediction of the partitioning between n-octanol/water (A Log Ps or Log P Kowwin ).
e lipophilicity of the compounds has the impact on the chromatographic analysis.Increase in the lipophilicity results in the longer retention of the compounds in the reversed-phase system both on the column and on the TLC plate.Ketoprofen and naproxen are the drugs that are characterized with lower Log P values which implies the faster elution than flurbiprofen and ibuprofen.In HPLC method reported by Gallo et al. [15], ketoprofen and naproxen were eluted from the column after ca.16 minutes; however, ketoprofen was eluted first.Faster elution of ketoprofen than naproxen was reported also by Zakeri-Milani et al. [16].Flurbiprofen and ibuprofen were eluted after 21-23 minutes.Guo et al. [17] reported also faster elution of ketoprofen enantiomers than flurbiprofen.e lipophilicity is a significant factor that influences the distribution of the substance in the human body.e values of Log P TLC for the analyzed compounds were also compared with their volume of distribution.It can be noticed that with the increase in the Log P TLC of the analyzed compounds, their volume of distribution increases.For ketoprofen which has the lowest Log P TLC value, the volume of distribution is 0.10 l/kg.e highest values are noticed for ibuprofen and flurbiprofen: 0.18 l/kg and 0.17 l/kg, respectively [18][19][20].
e lipophilic character of the compounds is also confirmed by the affinity of the drugs to bind with plasma proteins.All investigated compounds are the weak acids, and it results in binding with albumins [21].For ketoprofen, ibuprofen, flurbiprofen, and naproxen, the 98-99.9% of the drug is bound to the plasma proteins [21][22][23][24].e other barrier that drugs encounters in human body is blood-brain barrier (BBB).Ajome-Cat et al. reported that nonsteroidal antiinflammatory drugs crossed the BBB efficiently and they interact with microglial cell.ey inhibit the COX activity but also repress the expression of the genes associated with the microglial activation [25].Novakova et al. reported in in vitro tests that ibuprofen passes the barrier easily; however, it depends on the presence on the plasma proteins.e presence of 7.5% serum in in vitro model reduced the permeability of the drug [26].
e high lipophilicity of the investigated profens reflects also their use in the clinical practice.ey may be administered topically on the skin (naproxen, ibuprofen, and ketoprofen) as well on the mucous membranes (flurbiprofen).
e topical administration of the drug provides the targeted therapy, and the substance penetrates in the site of action and might be used by the patients which do not tolerate the nonsteroidal anti-inflammatory drugs orally [27].
e nonsteroidal anti-inflammatory drugs are divided into three categories due to their affinity to COX-1 and COX-2 [28].Knights et al. [28] reported that ibuprofen competitively binds to COX active site but dissociates rapidly from the active site of the enzyme.Its action towards  4 Journal of Chemistry COX-1 and COX-2 is rapid and reversible [29].On the other hand, flurbiprofen is a slow tight-binding molecule.It competes poorly at the beginning with the arachidonic acid but then binds tightly in a time-dependent manner.Both flurbiprofen and ibuprofen have the same conformation in the active site.However, they are in the different categories.e factor that differentiates them is the speed and efficiency binding to the aminoacids of the active site of COX [28].ese two drugs have the highest Log P values (Table 3) which may cause the high affinity to COX binding sites created by Arg120, Tyr355, and Glu524 [30].e investigated drugs possess the carboxyl group in their structure contrary to the selective COX-2 inhibitors which do not possess carboxyl moiety and the interaction with Arg120 is not necessary [28].e most lipophilic flurbiprofen has the highest affinity to COX-1 and COX-2 described by IC 50 .e reported affinity of the investigated drugs to COX-1 and COX-2 is different, and its activity differs between used cell lines [28].

Conclusions
e HPTLC technique is suitable for the determination of the lipophilicity of ibuprofen, ketoprofen, naproxen, and flurbiprofen.ere is a strong correlation between the Log P values obtained with n-octanol/water and chromatographic method and also with the Log P values calculated with a software.e lipophilicity of the compounds influences the physicochemical and biological properties.

Figure 1 :Figure 2 :
Figure 1: e molecular structures of the analyzed compounds.

Table 3 :
e R m0 , Log P, and Log P calc values of the investigated compounds.

Table 1 :
e chromatographic equations of the R m � f(c) function for the analyzed compounds.

Table 2 :
e R m0 and Log P values of the compounds with a known lipophilicity.

Table 4 :
[14]perimental values of Log P and the theoretical values of Log P[14]of the investigated compounds.
[12]lues reported by Pyka et al.[12]; Log P TLC , the Log P determined in our study with TLC technique; Log P oct/water , the Log P value determined with the n-octanol/water method.