Improvements in the Rheological Properties , Impact Strength , and the Biodegradability of PLA / PCL Blend Compatibilized by Electron-Beam Irradiation in the Presence of a Reactive Agent

In this study, we blended poly(ε-caprolactone) (PCL) into poly(lactic acid) (PLA) and compatibilized these PLA/PCL blends by electron-beam irradiation in the presence of a reactive agent to overcome drawbacks of PLA. To produce compatibilized blends, mixtures of the PLA/PCL/reactive agent were prepared using a twin-screw extruder and exposed to electron-beam radiation at room temperature. Glycidyl methacrylate (GMA) was chosen as the reactive agent to achieve interfacial cross-copolymerization between PLA and PCL phases. Morphological, rheological, and mechanical properties and biodegradabilities of blends were investigated. *e morphological study showed significantly improved interfacial adhesion for compatibilized blends, and this was supported by FTIR analysis and a rheological study. Impact strengths, thermal stabilities, and rheological properties of PLA/PCL blends were improved by compatibilization, and the biodegradabilities of compatibilized PLA/PCL blends were greater than that of pure PLA.


Introduction
Poly lactic acid (PLA) is an important bio-based and biodegradable polymer of high tensile strength and modulus, which now can be found in various industrial items, and is produced from nonfossil renewable resources by fermentation of polysaccharide [1].However, PLA has some drawbacks, which include low melt viscosity, low thermal stability, and brittleness, which limits its potential applications.Furthermore, although PLA is biodegradable, it degrades at a relatively low rate, and this leaves cope for improvement for industrial composting solid waste treatment.To overcome these shortcomings of PLA, we considered blending it with poly(ε-caprolactone) (PCL), which is a soft biodegradable polymer.However, as is the case for most polymer blends, PLA and PCL are immiscible and incompatible, and considerable efforts have been made to enhance compatibility between these two polymers to achieve acceptable PLA/PCL blends.As a result, many compatibilization strategies have been devised, such as reactive compatibilization or methods based on the addition of a polymeric compatibilizer [2][3][4][5][6][7][8][9][10][11][12][13][14].
e enzymatic hydrolyzability of PLA/PCL blend compatibilized by the addition of PLLA-CL decreased with increasing PLLA-CL content; however, tensile strength, Young's modulus, and elongation at break were increased by compatibilization.For the reactive compatibilized PLA/PCL blends, Semba et al. and Wang et al. [5,6] reported that the biodegradability and mechanical properties such as tensile strength, Young's modulus, elongation at break, and impact strength of blends were increased by reactive compatibilization.
Recently, exposure of polymer blends to high-energy radiation at room temperature has also been introduced to improve PLA/PCL compatibility [15][16][17][18].High-energy radiation has several advantages; for example, it provides continuous operation, requires little time, and causes less atmospheric pollution; curing is performed at ambient temperature, and because it enables process control, it offers design flexibility [19].
In this work, we blended PCL and PLA and compatibilized mixtures by exposing them to electron-beam irradiation (20 kGy) in the presence of glycidyl methacrylate (GMA) with the aim of reducing brittleness and increasing thermal stability, melt viscosity, and biodegradability of PLA.Morphological, rheological, and mechanical properties of compatibilized PLA/PCL blends were measured and compared with those of uncompatibilized blends.In addition, the biodegradabilities of compatibilized PLA/PCL (90/10) and (70/30) blends were determined under specific composting conditions [20].

Materials and Methods
2.1.Materials.PLA (NatureWorks ® PLA Polymer 4032D) of density 1.24 g/cm 3 and a melt flow index of 6.5 g/10 min (measured at 190 °C at a load of 2.16 kg) was purchased from NatureWorks LLC, and PCL (TONE-787) of the melt flow index 0.5 g/10 min was obtained from Dow/Union Carbide.Glycidyl methacrylate (GMA) and N,N-dimethyl formamide (DMF) were supplied by Sigma-Aldrich (WI, USA).

Preparation of PLA/PCL/GMA Mixtures.
e blend ratios of PLA to PCL of 90/10 and 70/30 (weight percent) were used in the study, and the amount of GMA content was fixed to 3 parts per hundred parts of resin (phr) based on the total mass of PLA and PCL.PLA, PCL, and GMA were mixed in a plastic bag before being extruded in a twin-screw corotating extruder (Ikegai, PCM-45, Japan).e extruder was operated at 200 rpm at a constant feed rate of 5 kg/h, and barrel and die temperatures were controlled at 190 °C and 185 °C, respectively.e extrudate was cooled in chilled water and cut into pellets of ˂1 mm diameter.e pellets were then dried for 24 hours at 50 °C prior to electron-beam treatment.PLA/PCL blends without GMA (uncompatibilized PLA/PCL blends) were also prepared for comparison purposes [21].

Electron-Beam Irradiation.
e pellets of PLA/PCL/GMA mixtures were irradiated using a commercial electron-beam accelerator (ELV−0.5,BINP, Russia, at a maximum beam current of 40 mA and beam energy of 0.5-0.7 MeV) under a nitrogen atmosphere.e irradiation dose was controlled by varying beam currents from 0.5 to 10 mA and conveyor speeds from 1 to 2 m/min.Radiation doses were measured by dosimetry (GENESYS 20, ermo SCIENTIFIC Co.) using dosimetry film (B3 WINdose Dosimetry, GEX Co.).e acceleration energy used was 0.7 MeV, and the effective penetration depth was ∼2 mm for a substrate of density 1 g/cm 3  [16,21,22].e compatibilized blends produced were dried in an oven at 50 °C for 12 hours to eliminate residual radicals.

Separation of Blends.
For FTIR analysis, compatibilized and uncompatibilized PLA/PCL (70/30) blends were separated into components using N,N-dimethyl formamide (DMF), which dissolves PLA but not PCL [6,23].e separation and purification was carried out as follows; first, a blend was immersed in DMF for 24 hours at room temperature.
e solution was then filtered, and the dissolved polymer was precipitated by adding water.To obtain high-purity precipitate, the process was repeated a second time.Unreacted GMA was removed because it is soluble in water.e precipitate so obtained was vacuum dried at 50 °C for 24 hours.
2.5.Characterization.Morphological analysis was carried out by scanning electron microscopy (SEM, Hitachi model s-4200, Japan) on cryofractured surfaces of blends.Infrared spectra were obtained by Fourier transform infrared spectroscopy (FTIR; Excalibur Spectrometer FTS 3000MX, Bio-Rad, USA) by grinding a constant weight of the dry sample with KBr and then pressing the powder mix to form transparent discs.Rheological properties were measured using a rotational rheometer (Physica MCR 30; Anton Paar GmbH, Germany).e equipment was run in parallel plate geometry with a plate of diameter 25 mm and a gap of 1.5 mm at 190 °C.Dynamic frequency sweep testing was performed at a strain of 2% in the angular frequency range 0.5 to 500 rad/s.Dynamic time sweep testing was also used to examine the thermal stabilities of blends at an angular frequency of 1.0 rad/s and a strain of 2% [24].
e mechanical properties of pure PLA and blends were determined using a tensile tester (Instron 4464; Instron, USA), and impact strengths were measured using an impact tester (Model 43-76; TMI Test Machines Inc., USA). e tensile and impact strength test specimens were prepared by injection molding (Nissei 35-oz, Japan) at a cylinder temperature of 200 °C and mold temperature of 40 °C.Tensile testing was performed at room temperature with a gauge speed of 10 mm/min and a gauge length of 25 mm.e tensile and impact strengths mentioned are the mean values of four independent tests.Biodegradability studies were performed as per ISO 14855 under aerobic controlled composting conditions [20,25].Biodegradability was defined as the percentage of carbon from test materials mineralized to carbon dioxide during composting.ree 3 liter vessels were used for each test material.Each vessel contained 30 g of the test material in a 300 g mature compost inoculum (Biocom, Kanglim Organic Company, Korea).e entire apparatus was placed in a dark incubator and maintained at 58 ± 2 °C for 45 days.Amounts of carbon dioxide produced from test materials were calculated by subtracting the total amount evolved in blank vessels containing only compost inoculum.

Morphologies of Blends.
e morphologies of PLA/PCL blends compatibilized or not by electron-beam treatment in the presence of GMA were compared.Figure 1 b) and 1(d)).In addition, the diameters of dispersed PCL particles were smaller in compatibilized blends, which were attributed to reduced interfacial tension difference between the two phases.ese morphological results indicate that interfacial adhesion between the PLA matrix and the PCL dispersion was substantially enhanced by electron-beam treatment, presumably caused by electronbeam initiated cross-copolymerization between PLA and PCL at PLA/PCL interfaces [21,26].

FTIR Analysis.
e FTIR analysis was performed to support the results of the morphology study, that is, to provide evidence of increased compatibility due to crosscopolymerization by electron-beam irradiation.Figure 2 presents the FTIR spectra (3200-650 cm −1 ) of pure PLA, pure PCL, and the separated substances from uncompatibilized and compatibilized PLA/PCL (70/30) blends.e spectrum of pure PLA (Figure 2 [27].e spectrum (Figure 2(c)) of the separated substance from the uncompatibilized blend was similar to that of the pure PLA (Figure 2(a)) and showed characteristic PLA bands, such as C�O stretching vibration at 1745 cm −1 and CH 3 stretching at 2994 and 2947 cm −1 .However, C�O stretching vibration of PCL at 1723 cm −1 and CH 2 stretching at 2864 cm −1 were absent.ese results indicate the absence of an interfacial reaction between PLA and PCL.On the contrary, the spectrum (Figure 2(d)) of the separated component from the compatibilized blend displayed strong absorption bands corresponding to C�O stretching of both PLA and PCL at 1746 and 1725 cm −1 , respectively, which were shifted a little to higher wave numbers compared to   those of pure PLA (1745 cm −1 ) and PCL (1723 cm −1 ).In addition, we also observed CH 2 and C-C (crystal phase) of PCL at 2864 cm −1 and 1293 cm −1 of PCL.ese FTIR results support that electron-beam-initiated cross-copolymerization had occurred in the presence of GMA at the PLA/PCL interface [21].
3.3.Rheological Properties.Dynamic rheological properties of polymers and their blends are highly dependent on polymer molecular eight and blend structure and compatibility [2,7,10,28].In the present study, we first determined the optimum radiation dose required to compatibilize PLA/PCL blends and then investigated the storage moduli and complex viscosities of the compatibilized blends prepared at optimum dosage.For this purpose, we prepared compatibilized PLA/PCL (90/10) blends irradiated at various doses (5−200 kGy) in the presence of GMA and then measured their complex viscosities in the angular frequency range 0.5-100 rad/s.Change in qualitative behaviors with radiation dose indicated that some chemical reactions, such as grafting, crosslinking, and chain scission, had been induced.As shown in Figure 3, the complex viscosity of the compatibilized PLA/PCL blend increased on increasing dosage to 20 kGy.However, blend complex viscosity decreased when the radiation dose was increased over 20 kGy, which was considered to have been caused by chain scission [19,29].In addition, complex viscosities of all compatibilized blends were higher than those of the uncompatibilized blend.Exceptionally, the compatibilized blend irradiated at 200 kGy had lower complex viscosity than uncompatibilized PLA/PCL blend in the high-frequency range due to decreased molecular weight and increased molecular weight distribution, which were caused by chain scission.Based on these results, we chose 20 kGy as the optimum dosage for preparing compatibilized PLA/PCL blends.
Figure 4 shows changes in the storage moduli of pure PLA, pure PCL, uncompatibilized blends, and blends compatibilized by electron-beam irradiation at 20 kGy.In the absence of increased interfacial adhesion, the storage moduli of PLA/PCL blends would be expected to lie between the values of the pure PLA and PCL.As shown in Figure 4, the storage moduli of uncompatibilized PLA/PCL (90/10) and (70/30) blends lay between those of pure PLA and PCL over the frequency ranges of 20−500 rad/s and 7−500 rad/s, respectively, but were higher than pure PLA and PCL below 20 rad/s (PLA/PCL (90/10) blend) and 7 rad/s (PLA/PCL (70/30) blend), respectively (arrows in Figure 4). is increase in the storage moduli of uncompatibilized blends might be caused by hydrogen bonding interaction between ester carbonyls and hydroxyls of PCL and PLA at the interface [30].However, modulus values between those of pure PLA and PCL at higher frequencies indicate that hydrogen bonding forces were negligible compared with dynamic shear force portion required for deformation [30]. is suggests that interfacial adhesion induced by hydrogen bonding was not enough to transfer exerted stress at the interface between PLA and PCL phases.Interestingly, below 2 rad/s, the modulus of the uncompatibilized PLA/PCL (70/30) blend was higher than that of the uncompatibilized PLA/PCL (90/10) blend despite a higher PCL content, presumably because total interfacial area had been increased, and thus, the amount of interfacial hydrogen bonding force had been increased.
In contrast to uncompatibilized blends, the storage moduli of all compatibilized blends were higher than those of pure PLA and PCL over the entire frequency range tested, indicating that compatibility of the PLA/PCL blend has been increased by electron-beam irradiation.In addition, high interfacial adhesion between continuous and dispersed phases can induce morphological stability by acting as a morphological network structure and thus increase blend moduli as is observed for cross-linked polymers.Moreover, the storage moduli of compatibilized PLA/PCL (90/10) and (70/30) blends were about 900 and 640 times higher, respectively, than those of pure PLA at an angular frequency of 0.5 rad/s.
Changes in the complex viscosities of pure PLA and PCL and of all blends as a function of angular frequency are shown in Figure 5. Pure PLA and PCL exhibited a long Newtonian viscosity plateau region and uncompatibilized PLA/PCL (90/10) and (70/30) blends showed weak shear thinning behavior.Complex viscosity of the uncompatibilized PLA/PCL (70/30) blend lays between those of pure PLA and PCL due to lack of compatibility, whereas that of the uncompatibilized PLA/PCL (90/10) blend was higher than pure PLA below an angular frequency 1 rad/s due to the effect of hydrogen bonding, as mentioned above.
For compatibilized blends, however, complex viscosities were much higher than those of pure PLA or pure PCL and showed strong shear thinning behavior over the whole frequency range.In fact, complex viscosities of compatibilized PLA/PCL (90/10) and (70/30) blends were 18 and 11 times higher than that of pure PLA at 0.5 rad/s, respectively.
ese rheological results provide additional evidence of enhanced compatibility due to electron-beam-induced cross-copolymerization between PLA and PCL [21].In addition, the enhanced melt complex viscosities and storage moduli of electron-beam-treated PLA/PCL blends would  Advances in Materials Science and Engineering make them suitable for applications, such as thermoforming, blow molding, and foaming, which require high melt viscosity and elasticity.

ermal Stability Investigation.
It is well known that thermal degradation reaction occurs during the melt processing of polymers and that this a ects their nal properties and those of their blends [2].us, the thermal stability of polymers is important for long-term melt processing, such as that required for extrusion processes.To investigate thermal stabilities of pure PLA and PCL, and of PLA/PCL blends, oscillatory complex viscosities were measured as a function of time at a strain of 2% and an angular frequency of 1 rad/s at 190 °C.Complex viscosities (η * ), normalized by their initial complex viscosities at t 0 (η * o ), are presented as a function of time for pure PLA and PCL and uncompatibilized PLA/PCL blends in Figure 6. e normalized complex viscosities (η * (t)/η * o ) of pure PLA and PCL decreased to 0.62 and 0.83, respectively, after 30 min indicating PCL was more stable than PLA during melt processing.
ese decreases corresponded to a loss in molecular weight caused by thermal degradation in the rheometer.Furthermore, the normalized complex viscosities of uncompatibilized PLA/PCL (90/10) and (70/30) blends were higher than that of pure PLA due to the blending of PCL.
To observe the e ect of compatibilization on the thermal stability of blends, we also normalized complex viscosities of compatibilized PLA/PCL (90/10) and (70/30) blends and compared them with those of uncompatibilized blends (Figure 7).e normalized complex viscosities of uncompatibilized PLA/PCL (90/10) and (70/30) decreased to 0.77 and 0.69, respectively, after 30 min at 190 °C, whereas those of compatibilized PLA/PCL (90/10) and (70/30) increased to 1.44 and 1.60, respectively.is increase in the normalized complex viscosities of compatibilized blends indicates improved thermal stability.Interestingly, the normalized complex viscosities of compatibilized blends were higher than 1.A similar result for the normalized complex viscosity of a compatibilized blend was reported by Al-Itry et al. [2].From these results, we conclude the thermal stability of PLA is improved by the addition of PCL and by the compatibilization treatment, which would provide more stable melt processing for industrial manufacturing operations.
3.5.Mechanical Properties. Figure 8 presents the impact strengths of pure PLA and PLA/PCL blends as determined by the notched Izod impact test.e impact strength of pure PLA was relatively low (28.8J/m) due to its brittleness [31].
e impact strength of uncompatibilized PLA/PCL (90/10) blend was 1.8 times higher (51.9 J/m) than that of pure PLA, whereas that of the uncompatibilized PLA/PCL (70/30) blend (31.9 J/m) was similar despite a higher content of soft PCL, which was probably due to the greater particle size of PCL than that of the uncompatibilized PLA/PCL (90/10) blend.As shown in Figure 1(c), the uncompatibilized PLA/PCL (70/30) blend had much larger PCL particles than the PLA/PCL (90/10) blend (Figure 1(a)) and had voids at interfaces between the PLA matrix and the PCL particles, which result in local stress concentration in a broader region, and this caused more rapid fracture progress [32].Interestingly, the impact strengths of compatibilized blends Advances in Materials Science and Engineering were markedly higher than those of uncompatibilized blends.More speci cally, the impact strengths of compatibilized PLA/PCL (90/10) and (70/30) were 2.9 and 3.1 times higher, respectively, than that of pure PLA. Figure 9 shows the tensile moduli of pure PLA and compatibilized and uncompatibilized PLA/PCL blends.For uncompatibilized blends, tensile moduli decreased as PCL content increased and were lower than that of pure PLA, whereas compatibilized blends had tensile moduli similar to that of pure PLA due to increased compatibility.

Biodegradability.
e ASTM de nes "biodegradable" as being "capable of undergoing decomposition into carbon dioxide, methane, water, inorganic compounds, or biomass, predominantly due to the enzymatic actions of microorganisms" [20].Figures 10 and 11 show the results of our biodegradability studies on pure PLA and PCL and compatibilized PLA/PCL (90/10) and (70/30) blends.e test apparatus was calibrated using cellulose as the reference material and was found to biodegrade by 91% after 45 days.Advances in Materials Science and Engineering e biodegradabilities of pure PLA and PCL were 22 and 65%, respectively, after the same period (Figure 10).Initial time lags (time required for the hydrolysis of polymer into diffusible oligomers before the onset polymer mass loss or microbial utilization) of pure PLA and PCL were approximately 14 and 2 days, respectively [20].
e lower biodegradability and longer initial time lag of pure PLA might be problematic when PLA is composted with easy compostable waste under industrial composting conditions [20,33].e biodegradabilities of compatibilized PLA/PCL (90/10) and (70/30) were increased to 41 and 50%, respectively, and initial time lags were 12 and 7 days, respectively.Cellulose and PCL had very high biodegradation rate at the earlier composting period, whereas PLA had very low biodegradation rate over the entire test period.Compatibilized PLA/PCL blends had high biodegradation rate after 20 composting days, presumably due to the fact that, in PLA/PCL blends, PCL degraded first to create holes which accelerated the degradation of PLA [34,35].

Conclusions
In this work, PCL was blended into PLA at the loading of 10 or 30% by weight to try to overcome the limitations (brittleness, low melt viscosity, thermal stability, and relatively poor biodegradability) of PLA.PLA/PCL blends were compatibilized by electron-beam irradiation in the presence of GMA.Morphology study showed that compatibilization diminished PCL (dispersed phase) particle sizes in blends and markedly reduced voids at PLA/PCL interface.FTIR analysis provided supporting evidence of an interfacial reaction between PLA and PCL.Furthermore, the storage modulus and complex viscosity of the compatibilized PLA/PCL (90/10) blend were about 900 and 18 times higher, respectively, than those of pure PLA at an angular frequency of 0.5 rad/s.Compatibilization increased the normalized complex viscosities η * (t)/η * o (t � 0)   of uncompatibilized PLA/PCL (90/10) and (70/30) from 0.77 to 0.69 to 1.44 and 1.60, respectively, and impact strengths of these compatibilized blends were 2.9-and 3.1-fold higher, respectively, than that of pure PLA. e biodegradability of the compatibilized PLA/PCL 70/30 blend was more than twice that of PLA (50% vs. 22%) after composting for 45 days.From these results, it can be concluded that blending PCL with PLA and electron-beam compatibilization in the presence of GMA reduced the brittleness of PLA and enhanced the storage modulus, melt viscosity, and thermal stability.In addition, the biodegradability of PLA was also increased by blending of PCL.It is believed these enhancements substantially increase the utilities of PLA/PCL blends.

shows the 2
Advances in Materials Science and Engineering SEM images of cryofractured surfaces of compatibilized and uncompatibilized PLA/PCL (90/10) and (70/30) blends.e SEM images of uncompatibilized blends (Figures 1(a) and 1(c)) showed voids between the dispersion phase (PCL) and the continuous phase (PLA) and the removal of some particles from the PLA matrix.On the contrary, no voids or PCL dislodgement was observed in compatibilized blends (Figures 1( (a)) showed absorption bands corresponding to C�O stretching vibration at 1745 cm −1 , CH 3 stretching at 2994 and 2947 cm −1 , asymmetric bending absorption of CH 3 at 1453 cm −1 , and C-O-C stretching at 1181, 1084, and 1043 cm −1 [27].On the contrary, the spectrum of pure PCL (Figure 2(b)) showed absorption bands corresponding to C�O stretching vibration at 1723 cm −1 , the CH 2 stretching at 2942 and 2864 cm −1 , and C-O and C-C (crystal phase) stretching absorption at 1293 cm −1