Detection of Enterocytozoon bieneusi in White Yaks in Gansu Province, China

Enterocytozoon bieneusi, the most common zoonotic pathogen of microsporidiosis, has been found in various animals and humans, but no information is available concerning the prevalence and genotypes of E. bieneusi in white yaks (Bos grunniens). In the present study, 353 faecal samples from white yaks in Tianzhu Tibetan Autonomous County, Gansu Province, Northwestern China, were collected and examined by PCR amplification of the internal transcribed spacer gene to estimate E. bieneusi prevalence and identify their genotypes. Of the 353 faecal samples, 4 (1.13%) were tested E. bieneusi-positive. Sequences analysis revealed that two known genotypes, namely, I (n = 1) and BEB4 (n = 2), and a novel genotype, namely, WCY1 (n = 1), were found in this study. Among them, genotype WCY1 was clustered into Group 1, and genotypes I and BEB4 belonged to Group 2. The present study firstly indicates the existence of E. bieneusi in yaks in Gansu Province, Northwestern China. This is also the first record of E. bieneusi in white yaks. Effective measures should be taken to control E. bieneusi infection in white yaks, other animals, and humans.


Introduction
Microsporidiosis is caused by Microsporidia, which have been recognized as opportunistic pathogens for humans and animals [1,2]. More than 1500 microsporidian species in 190 genera have been found since 1985 [3]. Among them, 14 species in 8 genera have been identified in humans [4]. Of these, Enterocytozoon bieneusi is the most common causative agent of human infection [5], with the symptoms of chronic diarrhea and other enteric disease [6].
At present, over 200 distinct genotypes of E. bieneusi have been identified based on ribosomal internal transcriber spacer (ITS) region of the rRNA gene [7]. These genotypes could be divided into several genetically isolated clusters, including a large cluster named as "the zoonotic genotypes" (Group 1) and some other groups, the so-called "host adapted groups" (Groups 2 to 9) [7]. It is surprising that some of the genotypes (I, J, and BEB4) in Group 2 have also been found in humans in recent years [8][9][10], so investigations of E. bieneusi prevalence in different hosts and assessing their zoonotic potential are important.
In China, the prevalence and genotypes of E. bieneusi have been reported in humans, some animals, and wastewater [11][12][13]. The yak is a unique bovine species and is a valuable, semiwild animal, living at high altitudes. The majority of yaks are distributed in China. As a unique yak breed, the white yak lives only in the Tianzhu Tibetan Autonomous County (TTAC), Gansu Province, Northwest China. However, no information regarding E. bieneusi prevalence in white yaks is available. Therefore, the objective of the present study was to estimate prevalence and genotypes of E. bieneusi in white yaks in China.  Province, Northwest China, were collected. One faecal sample was collected from each examined white yak, and the basic information was recorded in Table 1. All samples were collected using a sterile disposable glove and then were stored in box with ice immediately and sent to the laboratory.

Sequencing and Phylogenetic
Analyses. The positive secondary PCR were sequenced by Genscript Company (Nanjing, China). The sequences were confirmed by the program Clustal X 2.0 and aligned with reference sequences of E. bieneusi available in GenBank to determine the genotypes. Neighbor-joining (NJ) method was used to reconstruct the phylogenetic tree, and bootstrap analysis was performed using 1,000 replicates ( Figure 1). The novel genotype(s) of E. bieneusi was named based on the established nomenclature system.

Nucleotide Sequence Accession Numbers.
The representative nucleotide sequences of this study have been deposited in the GenBank database under accession number KU598221-598223.
In this study, three ITS genotypes (two known genotypes and a novel genotype) were identified in white yaks in China, with BEB4 being the predominant genotype, which is consistent with that reported in black yaks in Qinghai [6]. The present study suggests that the three genotypes (I, BEB4, and WCY1), identified in this study, together with the genotypes J, CHN11, and CHN12, previously found in black yaks in Qinghai, are endemic in yaks in China. Both the known genotypes BEB4 and I identified in this study were also previously reported in nonhuman primates (genotypes BEB4 and I) and cats (genotype I) in China [19]. Sequence analysis indicated that the novel E. bieneusi genotype (WCY1) is closely related to genotype GD-9 (KF305586) from cynomolgus monkey, with three nucleotide differences, and it has four nucleotide differences relative to the sequence of genotype PSD-16 (KJ668720) from dog. The remaining genotypes (KU598222 and KU598223) have identical sequences to the reference genotypes BEB4 (AY331008) and I (KT984486) available in GenBank. Of the three genotypes, genotype WCY1 belonged to Group 1, which implied that the white yaks may be a potential source of human infection. Although the remaining genotypes I and BEB4 were classified into Group 2, we must be cautious because the cattle-specific genotypes (I, BEB4, and J) have been found in humans [8][9][10]. In conclusion, the present study demonstrates the occurrence of E. bieneusi in white yaks for the first time. Moreover, the present study also indicates that the genotypes I, WCY1, and BEB4, found in this study, together with the genotypes J, CHN11, and CHN12, previously identified in black yaks in Qinghai, are endemically prevalent in yaks in China.

Conflicts of Interest
All the authors declare no conflicts of interest.