The Maternal Brain: An Organ with Peripartal Plasticity

The time of pregnancy, birth, and lactation, is characterized by numerous specific alterations in several systems of the maternal body. Peripartum-associated changes in physiology and behavior, as well as their underlying molecular mechanisms, have been the focus of research since decades, but are still far from being entirely understood. Also, there is growing evidence that pregnancy and lactation are associated with a variety of alterations in neural plasticity, including adult neurogenesis, functional and structural synaptic plasticity, and dendritic remodeling in different brain regions. All of the mentioned changes are not only believed to be a prerequisite for the proper fetal and neonatal development, but moreover to be crucial for the physiological and mental health of the mother. The underlying mechanisms apparently need to be under tight control, since in cases of dysregulation, a certain percentage of women develop disorders like preeclampsia or postpartum mood and anxiety disorders during the course of pregnancy and lactation. This review describes common peripartum adaptations in physiology and behavior. Moreover, it concentrates on different forms of peripartum-associated plasticity including changes in neurogenesis and their possible underlying molecular mechanisms. Finally, consequences of malfunction in those systems are discussed.


Introduction
In all mammalian species the peripartum period is one of the most plastic periods throughout a female's life. During pregnancy and lactation, numerous changes on the physiological, cellular, and molecular level occur, which particularly distinguish a lactating mother from a nulliparous female and which prepare the female for the challenges of motherhood. Those dramatic changes in maternal physiology, plasticity of the maternal brain, and maternal behavior will not only help to ensure the survival of the offspring, but also act in concert for physiological and mental health of the mother [1][2][3][4]. However, the peripartum period represents also a time of high risk for women to develop physiological and mental disorders that are particularly associated with those peripartum adaptations. Thus, 0.5-5% of pregnant women will develop preeclampsia after 20 weeks of pregnancy [5,6] and about 18% will be diagnosed with gestational diabetes between week 24 and 28 of pregnancy [7]. A varying high percentage of women will also be affected by perinatal mental disorders such as postpartum blues (30-75%) [8], the more long-lasting postpartum depression (10-22%) [9,10] and postpartum anxiety (5-12%) [11,12], or in even more serious cases postpartum psychosis (1-2%) [13]. Although some of the physiological adaptations that occur throughout pregnancy and lactation are well known, the mechanisms underlying the above-mentioned medical conditions are largely unexplored. Also, comorbidities of mental and of somatic disorders which might have originated during pregnancy are, in our opinion, underestimated. Therefore, the following review will describe the common adaptations that occur on the physiological, molecular, and behavioral level during the sensitive period around and after pregnancy and describe regulatory mechanism and potential causes for peripartumassociated disorders.

Physiological and Molecular Adaptations during the Peripartum Period
One of the first essential steps to ensure the proper development and survival of the future offspring is placentation and thereby the formation of the fetoplacental unit. Placentation is a two-stage process of coordinated invasive vasculogenesis (i.e., the formation of a branching network of vessels with chorionic villi of fetal origin) and later angiogenesis (i.e., the modification of the existing vascular network) [14][15][16]. The key event for a proper placental development and for pregnancy to proceed normally is the invasion of the decidual stroma of the maternal spiral arteries by the fetal cytotrophoblast [17]. After invasion, the cytotrophoblast will secrete angiogenic factors like vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) [18]. VEGF is generally expressed by all cells of the fenestrated endothelium and stimulates the proliferation and survival of endothelial progenitor cells (EPCs). Given the enhanced needs in maternal blood supply during pregnancy, it is obvious that VEGF represents a crucial factor particularly during that time by stimulating vasculogenesis and angiogenesis. Inducing a generalized vasodilatation VEGF ensures the integrity of the maternal endothelium but moreover plays an important role in the proper placental development. In more detail VEGF promotes vasculogenesis and transformation of the maternal spiral arterioles from small caliber resistance vessels to large caliber capacitance vessels. This remodeling is essential for the adequate perfusion of the fetoplacental unit and consequently the exchange of nutrients, oxygen, and waste between the mother and the developing fetus (for review see [19,20]). Although the function of PlGF during pregnancy is not fully understood yet, it seems to act to amplify VEGF-induced processes described above [21]. Aside from placental factors regulating vasculogenesis, some cytokines have been shown to be essential for pregnancy-associated events like trophoblast invasion and vasculogenesis (for review see [22]). Indeed, pregnancy is characterized by fundamental changes in maternal cytokine levels, to enable the survival of the fetus, which may be viewed as a semiallogenic graft [23]. Thus, pregnancy might be considered as a state of mild, controlled inflammation, however, ensuring the maintenance of a delicate balance between antiinflammatory (i.e., Il-4 and IL-10) and proinflammatory (i.e., TNF , IL-1, IL-6 and IL-8) cytokines.
Aside from these changes that occur during early stages of pregnancy, there are numerous adaptations at the level of the mother's brain towards the end of pregnancy and into the period of lactation. Respectively, the response of the hypothalamic-pituitary-adrenal (HPA) axis to a variety of stressors has been shown to be severely attenuated in mothers [24,25]. The reduced peak HPA axis activity is predominantly the result of numerous central changes in excitatory and inhibitory pathways mainly within the hypothalamus. Several animal studies revealed that the pattern of excitatory inputs to the hypothalamus is altered during pregnancy [26]. Hence, a reduction in the noradrenergic tone within the nucleus paraventricularis (PVN) and a reversed opioidergic system contribute to inhibit the HPA axis activity around birth [26][27][28][29] (and see [30][31][32][33][34] for review). Furthermore, CRH mRNA expression in the PVN as well as CRH binding in the adenohypophysis is markedly reduced during pregnancy and lactation, leading to a diminished CRH production and release by PVN neurons [28,[35][36][37][38]. In spite of the dampened action of those excitatory systems, inhibitory systems like the oxytocin (OXT) and prolactin (PRL) system are highly activated during the peripartum period. Accordingly, OXT and PRL mRNA expression, OXT-receptor (OXT-R) and PRL-receptor (PRL-R) expression in the PVN and nucleus supraopticus (SON) [39][40][41][42], and OXT release [43][44][45] are increased during that time. In addition, the hypothalamic oxytocinergic system undergoes fundamental structural and functional reorganization with respect to dendritic branching and synaptic plasticity (for details, see Section 3). Clearly, peripartum-associated changes in the OXT and PRL system are essential in mediating reproductive functions such as the promotion of labour, lactogenesis, milk ejection, and maternal behavior [46][47][48][49]. Furthermore, those changes are a crucial feature to protect the late pregnant and lactating mother from overresponding to stressors (see [1-3, 34, 50, 51] for review).
Contrary to the discussed attenuated HPA axis response to physiological and psychological stressors, several mammals, including humans, rats, mice, and sheep, show an increase in basal circulating glucocorticoid levels during lactation [35,[52][53][54]. This lactation-associated hypercortisolism/hypercorticism might be due, at least in part, to an increased expression of vasopressin (AVP) in the PVN of the hypothalamus [37] and a simultaneous enhanced sensitivity of the pituitary to this neuropeptide [28].

Structural, Functional, and Molecular Plasticity of the Brain during the Peripartum Period
During the peripartum period the maternal brain undergoes multiple macroscopic, microscopic, cellular, and molecular changes. It is not surprising that brain regions that are particularly affected by peripartum-associated modifications are mostly those that can be summarized as the "maternal circuitry. " Some of these brain regions are crucial for the onset, maintenance, and regulation of maternal behavior (i.e., nest building, grooming, and protection of the young); others control memory, learning, and responses to fear and stress. One part of this "maternal circuitry" is the maternal motivational system, which has been nicely described by Numan in "motivational systems and the neural circuitry of maternal behavior in the rat" [55]. Briefly, Numan describes the hormonal-primed medial preoptic area (MPOA) with the adjacent bed nucleus of the stria terminalis (BNST) as central region to induce the onset of maternal behavior by suppressing fear responses to pup odors on the one hand and activating the nonspecific motivational system (i.e., the mesolimbic dopamine system) on the other hand. Brain regions involved in the avoidance of pups as seen in virgin rats are the main and the accessory part of the olfactory bulb (OB), the medial amygdaloid nucleus (MeA), the anterior hypothalamic nucleus (AHN), and the periaqueductal grey (PAG). The nonspecific motivational system is composed by the ventral tegmental area (VTA), the nucleus accumbens (NAc), and the ventral pallidum (for review see [55]). Further important brain regions that can be assigned to the "maternal circuitry" include the hypothalamic nuclei, the PVN, and SON, which are central for the regulation of anxiety and stress and the maintenance of maternal behavior (see [1,34,56] for review). Neurons of the hypothalamus project centrally to the limbic system, that is, the hippocampus, which interconnects to regions of the frontal lobe, that is, the medial prefrontal cortex (mPFC). Whereas the hippocampus is crucially involved in learning and memory processes not only in the context of pregnancy and lactation, the mPFC seems to be central for the perception, appraisal, and the regulation of peripartum-relevant stimuli and acts in concert with the hippocampus to regulate cognition during the peripartum period [57] ( [58,59] for review).
The following chapter concentrates on peripartumassociated changes in neural-glial interactions, synaptic plasticity, dendritic morphology, and adult neurogenesis in the selected above-mentioned brain regions.

Peripartum-Associated Volume Changes of the Brain.
Clearly, one of the most easily observable changes that occur during the peripartum period is a change in maternal brain size, which has been shown both in humans [60] and rodents [61]. In their clinical study, Oatridge et al. recruited a total of nine healthy (control) mothers and five preeclamptic (PE) women. By analyzing brain volume via T1-weighted MR before pregnancy (controls), during pregnancy (controls), shortly before delivery (PE), six (PE) and 52 weeks postpartum (control and PE), they were able to show that brain size was significantly reduced whereas the lateral ventricular size was increased in both groups, respectively. This effect, which started with placental implantation and reached its maximum at term, has been shown to be even more pronounced in mothers that suffered from preeclampsia during pregnancy [60]. The observed peripartum changes in brain size seem to be interspecific, as we recently showed that absolute and relative brain weight are reduced on lactation day (LD) 14 in rats, reflecting the results in humans. In more detail, we revealed that hippocampal volume is significantly smaller in lactating compared to nulliparous females. Interestingly, the mentioned lactation-associated effect on brain weight and hippocampal volume was reversed, when rats were exposed to chronic restraint stress between LD2 and LD13 [61]. Unfortunately, the physiological importance of the abovementioned findings in humans and rodents are not well understood at present and the underlying mechanisms still need to be elucidated.
Volume changes have not only been reported in the hippocampus, but also in other brain regions with a significant role during the peripartum period. Correspondingly, the volume of the pituitary underlies pregnancy-and lactationassociated changes. Although the pituitary enlarges during the course of pregnancy, probably due to hyperplasia of PRL cells [62], it decreases up to eight month after delivery in humans [63][64][65] or, respectively, seven days after delivery in rodents [66][67][68], when the number of PRL cells reaches prepregnancy levels [69]. Similar effects have also been observed in the MPOA and SON of pregnant/lactating rats. Respectively, cell body size (referring to soma and perikaryon) of MPOA neurons has been shown to be increased in late pregnant rats when compared to ovariectomized or diestrus rats. Interestingly, treatment with the pregnancy-mimicking regimen of progesterone and estradiol induced the same changes as seen during natural pregnancy. Given this and the fact that the area of the soma returned to prepregnancy levels with the onset of lactation [70] shows the impact of pregnancy and its attendant hormonal exposure on these changes, while during lactation the cues from pups seem to primarily maintain maternal motivation. Likewise to the pregnancy-associated changes in MPOA, the SON of lactating rats increases in volume due to hypertrophy of OXT somata and dendrites [71][72][73].
Morphological adaptations have furthermore been observed in another hypothalamic structure, namely, the PVN. The group around Cortés-Sol analyzed the inner capillary diameter (ICD) of 800 capillaries from magnocellular and parvocellular regions of the PVN in diestrus nulliparous female rats or at 2 weeks of lactation. In this study they were able to show that nulliparous rats presented mostly capillaries with small ICD, whereas lactating rats exhibited capillaries with larger ICD. Interestingly, the space occupied by the neurovascular compartment, such as neurons, astrocytes, and other glial cells, did not change with lactation [74], suggesting that peripartum-associated changes in angiogenesis do not exist at the level of the PVN, at least not in lactation. However, alterations with long-term impact on the neurovascular compartment might occur earlier during the peripartum period. Respectively, human studies have shown a significant increase in the number of circulating EPCs with the progression of pregnancy [75], an effect that was absent in women with preeclampsia [76,77]. This is an important finding, as the peripartum-associated changes in EPCs and the microvasculature of the PVN might play an important role in enhanced availability of the neurohypophyseal hormones AVP and OXT during the peripartum period by increasing their cytoplasmatic transport from the luminal to the abdominal side of the membrane. Indeed, OXT plasma concentrations have been shown to be diminished in women with gestational hypertension [78]. As hormones like AVP and OXT have been shown to be implicated in mechanisms of cell volume regulation in rodents [79], it might be that PVN OXT neurons modulate their own capillary blood supply and vice versa. Given the fact that the OXT system in the hypothalamus undergoes intriguing morphological plasticity during the peripartum period, which will be discussed in one of the following paragraphs, such a mechanism seems to be indeed very likely.

Peripartum-Associated Receptor Plasticity in Different
Relevant Brain Regions. During the peripartum period the maternal brain undergoes marked changes in receptor expression. Unsurprisingly, brain regions and neuronal systems that are affected the most by these alterations are those known for their importance in different aspects of maternal behavior. With their rat model of high licking/grooming (LG) and low LG rats, Meaney and coworkers have significantly contributed to a better understanding of the correlation between maternal care and receptor plasticity in different neuronal systems. In a couple of elegant studies they did not only reveal that an increase in estrogen-receptor (ER ) expression in the MPOA on LD6 was correlated with an increased level of LG behavior in the high LG group, but also that the maternal behavioral phenotype was epigenetically transmitted over generations by a cytosine methylation process across the ER 1b promoter [80,81]. Interestingly, the level of ER expression seems to occur as a function of reproductive experience as seen in mice, rats, and sheep [82][83][84]. In more detail, ER expression in the MPOA, but also the MeA, the PVN, and SON has been shown to be increased four days before parturition in multiparous ewes [84], which might explain the increased estrogen responsiveness to induce maternal behavior in multiparous dams [85]. The effect of parity on ER expression seems to be long-lasting, as an increase in the MPOA and MeA has been observed in middle-aged cycling females several month after pregnancy [82].
Similar to ER , OXT-R expression levels have been found to be increased in several distinct "maternal" brain regions like the MPOA, VMH, BNST, lateral septum (LS), central amygdala (CeA), and the PVN of rodents (see [86] for review). The observed alterations in OXT-R distribution pattern reflect the crucial role of the OXT system to orchestrate different aspects of maternal behavior, including maternal care. Indeed, there is a close relationship between maternal care and OXT-R mRNA expression levels in those brain regions. Accordingly, dams characterized by a high maternal responsiveness to pups (i.e., high LG) have significant higher OXT-R levels in the MPOA, the CeA, the LS, and the BNST compared to mothers that show low levels of LG behavior [87]. Importantly, lowering OXT-R expression by repeated exposure to restraint stress between pregnancy day (PD)15 and PD21 or inhibiting OXT-R action by administration of an OXT-R antagonist on LD3 eliminated the described differences in maternal behavior [87,88]. Similarly, hypothalamic OXT-R expression has been shown to be essential for the lactation-associated anxiolysis, as central application of an OXT-R antagonist in the PVN increased anxiety in lactating but not virgin or male rats [44]. Given the fact that both OXT and PRL are key players during the peripartum period, acting synergistically to regulate maternal care, anxiety, and stress, it is not surprising PRL-R expression is comparably altered during that time. An upregulation of PRL-R mRNA has been shown in many brain regions known for their importance in regulating maternal behavior like the MPOA, the BNST, and VMH during different timepoints within the peripartum period (i.e., PD12, 2 h postpartum, LD7-10) [89,90]. Marked changes have been found in the PVN and the SON of rats, where the proportion of OXT neurons expressing the long form of the PRL-R was significantly increased during pregnancy and lactation [41], providing evidence that PRL directly and specifically regulates the activity of OXT neurons. Likewise to the above described studies regarding the OXT system, lowering PRL-R activity by chronic administration of PRL-R antisense oligonucleotide increased anxiety-related behavior and inhibited the PRL-mediated attenuated responsiveness of the HPA axis, thus explaining the parallel stress-hyporesponsiveness during lactation [91,92]. Aside from the regulatory role of receptor plasticity in the stress-induced HPA axis activity, peripartum-associated alterations in receptor expression also seem to be essential in the dampened sensitivity of the adrenocortical negative feedback during that time. Indeed, in vitro binding assays using 3[H] dexamethasone as radioligand revealed a reduced glucocorticoid receptor binding capacity in the hippocampus of rats during the first two weeks of lactation [93].
Aside from the onset and display of maternal care, altered anxiety, and stress responses during lactation, the expression of maternal aggression is an important feature ensuring sufficient protection and thus survival of the offspring. Studies in rats revealed that peripartum-associated fluctuations of the vasopressin receptor V1a (V1a-R) in brain regions of the "maternal circuitry" are substantial for the timely and finetuned expression of maternal aggression. Accordingly, the level of V1a-R binding in the BNST, MPOA, LS, and CeA has been shown to be positively correlated with the level of aggressive behavior throughout pregnancy, parturition and lactation [94]. Further proving the specific importance of the peripartum-associated V1a-R upregulation in the control of maternal aggression, bilateral infusion of a selective V1a-R antagonist into the BNST (daily between LD1 and LD6) significantly reduced maternal aggression, while it did not affect the frequency of arched back nursing [95].
In summary, the multiplicity of studies revealing changes in receptor expression of hormonal systems in specific maternal brain regions highlights the importance of these changes during the peripartum period. Together with other peripartum-associated forms of plasticity as will be discussed in the following sections, such changes in receptor expression act synergistically to positively influence the behavioral repertoire, physiological and psychological wellbeing of the mother, and thus survival of the offspring.

Peripartum-Associated Synaptic Plasticity of the OXT System in the Hypothalamus.
Classically, synaptic plasticity such as long-term potentiation or depression is defined as longlasting functional modification within preexisting synapses [96]. However, synaptic transmission can also be significantly altered by structural synaptic plasticity. During the peripartum period, this form of synaptic plasticity occurs in the hypothalamo-neurohypophysial SON and magnocellular PVN, when OXT neurons undergo an extensive neuronal and glial remodeling [97]. Thus, it has been shown that under conditions that stimulate OXT release, such as parturition and lactation, there is an increase in the numerical density of axosomatic and axodendritic synapses on OXT neurons in the SON as seen in tissue of rats that have nursed their pups for 11 days [98]. These changes, which occur rapidly within 24 h of stimulation [99] ( [100] for review), are concomitant with a glial retraction of OXT somata and dendrites leading to a juxtaposition of a significant portion of the plasmalemma [73,101,102]. The reduction in the astrocytic coverage of postsynaptic elements is an essential prerequisite for the above mentioned formation of new synaptic contacts during the peripartum period and alters neuronal function directly via the modification of synaptic transmission and indirectly by preparing neuronal surfaces for synaptic turnover. Once stimulation is over, astrocytic processes will again cover the OXT surfaces as seen under basal conditions in different adult neuronal tissues (see [103] for review). Aside from the peripartum-associated changes in the number of synapses, there have also been rodent studies revealing an increase in the number of shared synapses in OXT neurons [71,97,100,102], further amplifying the synaptic input. As well as for the above-mentioned changes in the number of spines, the synaptic coupling of OXT neurons takes place rapidly within two hours after stimulation [104]. Another important aspect of synaptic plasticity in the hypothalamus that occurs with the onset of lactation is a remodeling of afferent inputs that control the activity of the OXT system. Although under basal (i.e., nonlactating) conditions about 35% of synapses in the SON are GABAergic [71], 22% are glutamatergic [71], and 10% are noradrenergic [105], this distribution changes during the stimulatory influence of lactation. Hence, analysis of brain sections from lactating rats that had suckled litters for at least 10 days revealed that there is an increase in axosomatic and axodendritic GABAergic synaptic contacts, accounting for about 50% of all SON synapses in lactating rats [71,98]. The origin of the GABAergic input to the magnocellular neurons arises from local interneurons [106,107], as well as from adjacent hypothalamic areas [108,109]. Although, the origin of the glutamatergic afferents is comparable with those of GABA, morphological remodeling is less pronounced in glutamatergic synapses with only a 3% increase compared to basal conditions [71,102]. Although, noradrenergic innervation of OXT and AVP neurons is equal in nulliparous females, there is a significant increase in noradrenergic innervations of OXT neurons during lactation [105]. The fact that finetuned morphological plasticity in the OXT system appears fairly rapid and disappears once stimulation is over raises of course the questions of its functional implications. It has been speculated that the increased number of synaptic contacts as well as an increase in both inhibitory and excitatory inputs is an important mechanism for the unique pattern of electrical activity that characterizes OXT neurons during lactation, that is, their pulsatile firing ability, leading to the bolus release of OXT (see [110,111] for review). Furthermore, increased inhibition by GABA might be needed to ensure that activation of OXT neurons only occurs by lactationrelevant stimuli, but not others. Indeed, this possibility seems to be likely, as stress-induced stimulation of OXT neurons by injection of hypertonic saline has been shown to be reduced during the peripartum period [112].

Peripartum-Associated Neuronal Plasticity in the Hippocampus and the SVZ/OB. Another important contribution
to maternal neuroplasticity is provided by the mechanism of adult neurogenesis. Although the production of new neurons throughout adulthood has been suggested in several brain structures including the neocortex, piriform cortex, amygdala, striatum, substantia nigra, dorsal vagal complex, and the hypothalamus of mammals (see [113,114] for review), peripartum-associated changes have only been revealed in the two main neurogenic regions, namely, the subventricular zone (SVZ) of the lateral ventricles and the subgranular zone (SGZ) of the hippocampal dentate gyrus (DG). The SVZ gives rise to neuroblasts migrating a fairly long distance via the rostral migratory stream (RMS) to the OB, where they differentiate in one of the two main types of interneurons in the OB, granule cells (GC), or periglomerular neurons. Stem cells in the granule cell layer of the DG only migrate a short distance after proliferation and differentiate to one of the three main cell types in the hippocampus, neurons, astrocytes, or oligodendrocytes.
To date, the functional implication of adult neurogenesis during the peripartum period is not fully understood; however, given the fact that newly generated cells fully integrate in mature preexisting circuits it is likely that they significantly contribute to an enhanced plasticity and responsiveness to specific stimuli in a new environment with changing demands during this susceptible time. The following paragraph will discuss the state of the art of peripartum-associated alterations in adult neurogenesis and their possible contribution to maternal behavior and vice versa. The underlying regulatory mechanisms will be highlighted in Section 5.
Numerous studies have been performed during the last decade, increasing the understanding of the mechanisms controlling adult neurogenesis and its functional implications during pregnancy and lactation. Taking the broad consensus of all these studies it appears that the peripartum-associated changes in adult neurogenesis are not only dependent on the neurogenic region looked at, but also are species-and time-dependent. Hence, cell proliferation in the SVZ has been shown to be increased on PD7 in mice, but not at later time points during pregnancy (i.e., PD14 and PD21) [115,116], whereas in rats the complete opposite picture occurred [117]. The observed differences might be explained by methodological differences, that is, the number of BrdU injections and the amount of BrdU injected [118]. However, the species-dependent discrepancy might also be due to the level of functional importance the olfactory system plays in relation to maternal behavior in both species. The observed increase in cell proliferation on PD7 might lead to the integration of a greater number of newly generated interneurons at parturition, when the olfactory demands are high and might thereby contribute to the onset of maternal behavior in mice. Inhibition studies seem to be an applicable, insightful tool to investigate the importance of SVZ/OB neuroplasticity in relation to maternal behavior. However, as the methods disrupting neurogenesis differ a lot with respect to their specificity and thus to their extent in outcome, care must be taken when interpreting their results. Accordingly, it is not surprising that partially controversial results that are achieved are dependent on the approach used. By genetically ablating newly born neurons in the forebrain of mice Sakamoto et al. were able to show that new mothers showed abnormal maternal behavior and significant impairments in retrieval behavior, leading to the death of pups within 24-72 h after delivery [119]. Similar results were achieved by the group of Larsen who used the antimitotic drug bromocriptine to inhibit SVZ mitogenesis in PD7 mice, consequently reducing 6 Neural Plasticity the number of neurons in the OB on LD2. Although, retrieval behavior in the home-cage was unaffected, pup retrieval was severely impaired when the mothers were tested in a novel environment [116]. It has to be kept in mind though that both genetically targeted ablation and the use of antimitotic drugs lack specificity of targeting neurogenic regions. Thus, it is not precluded that the observed changes in behavior seen in those studies are due to disruption of processes in other neurogenic regions like the hippocampus. Contrary to the above-mentioned results, a study using focal gamma irradiation which specifically disrupts neurogenesis in the SVZ/OB circuitry did not result in an impairment of maternal behavior [120]. Given the fact that adult-born neurons are fully responsive to odor stimuli at two weeks of age [121] and the fact that olfaction plays a key role in the establishment of maternal behavior, learning and bonding in mammals (including mice, rats, sheep, goats, and humans) [122] (and see [123,124] for review) suggests that the integration of adult-generated neurons in the preexisting olfactory circuitry might be rather required for odor learning and distinguishing than for maternal behavior itself. However, given the varying effects observed, further studies are highly desired to finally elucidate the contribution of peripartum-associated changes in SVZ/OB neurogenesis with relation to maternal behavior.
Although, the importance of pregnancy-stimulated neurogenesis in the SVZ with respect to lactation has been beautifully demonstrated by the studies of Shingo et al. [115], there is no proof so far that lactation itself would affect adult neurogenesis in the olfactory system in rodents. However, it seems that pup presence/maternal behavior has the potential to induce lactation-associated changes in SVZ neurogenesis in sheep and rodents. Respectively, a stimulatory effect of interaction with the young has been observed in sheep during the early postpartum period [125]. Comparable observations were made by Furuta and Bridges in their experiments in rodents, when they exposed nulliparous female mice daily to foster pups in order to induce maternal behavior. When they compared maternal nulliparous females with those in which daily fostering was not efficient to induce maternal behavior, they found an increase in the number of BrdU+/NeuN+ double-labeled neurons in the SVZ of the former group [126]. Interestingly, comparing studies analyzing olfactory and/or hippocampal neurogenesis suggests that parturition and interaction with the young affects both cell proliferation and cell survival in a different manner across the neurogenic zones. Thus, contrary to the documented results regarding adult neurogenesis in the OB, there is little or conflicting evidence that pregnancy is associated with changes in hippocampal neurogenesis. Although BrdU+ cells and BrdU+/DCX+ cells have been shown to be reduced in mice on PD14 [127] and differentiation and migration of adult generated neurons have been shown to be increased in a rat model of hormone-simulated pregnancy [128], no changes in cell proliferation have been reported in mice and rats during early [115,117,129] or other late stages [117,129] of gestation. However, there is a significant change in hippocampal neurogenesis during the postpartum period. In all species studied so far, cell proliferation is severely diminished during early and midlactation, an effect that is restored by the time of weaning [61,125,[130][131][132]. Similarly, one-week survival of newly born cells has been shown to be decreased, whereas two-week survival was unchanged in lactating rats [131]. Whether cell survival is altered at parturition or the first days postpartum, as seen in the OB is not known so far. In summary it seems that maternal experience, but particularly the presence of pups, has a suppressive, albeit temporary effect on hippocampal neurogenesis, as nicely revealed in lactating female rats. Respectively, removal of pups immediately after birth, thus preventing nursing and the associated rise in CORT levels, restored cell proliferation in dams to the level of nulliparous females [131]. Interestingly, the observed effect may not be based on nursing alone, as similar results have been observed in male California mice, a species known to show biparental care. In more detail, Glasper et al. showed that hippocampal neurogenesis was reduced at the time of weaning in parenting fathers when compared to nonparenting fathers [133]. However, the effect of parenting on hippocampal neurogenesis in males seems to be highly species dependent as other studies in prairie voles and inbred C57BL mice showed a stimulatory effect of parenting on neuron production [134,135]. Given the fact that environmental enrichment is typically believed to have beneficial effects on neuroplasticity (for review see [136]) and the fact that maternal/paternal experience implicates such enrichment, the results regarding a reduction in hippocampal neurogenesis at the first glance seem to be surprising. However, aside from pup presence the main stimulus for the observed changes are the actual physiological conditions associated with the peripartum period, as nulliparous rats that were exposed to pups actually showed an increase in cell proliferation [132]. The detailed molecular mechanism underlying peripartum-associated alteration in SVZ/OB and hippocampal neurogenesis will be the subject of Section 5 and we would kindly refer the reader to the respective section. To date the exact functional contributions of maternal experience-induced changes in hippocampal structure are largely unknown; however, the fact that being a mother can remodel neuronal systems in such extent indicates that they play an important role during this sensitive time frame and might be fundamental for the maternal physiological and mental health (see also Figure 1 for graphical presentation of peripartum-associated changes in hippocampal neurogenesis.)

Peripartum-Associated Changes in Dendritic Morphology and Spines in Different Relevant Brain
Regions. The effects of parenting on neuronal plasticity are not only limited to alterations in cell proliferation and survival, as discussed above, but also extend to complex morphological changes, including alterations in the number and density of spines, as well as dendritic architecture.
Over the last decades, several studies in rodents revealed peripartum-associated alterations in different relevant brain regions like the hippocampus, the OB, the mPFC, and the MPOA, as well as the PVN and the SON of the hypothalamus. Thus, pregnancy has been associated with a reduced complexity of CA3 pyramidal neurons of the hippocampus as seen in rats [129]. Although CA1 pyramidal neurons do not show these pregnancy-dependent changes [129], they are altered during lactation as after weaning. Respectively, primiparous rats exhibit a reduction in dendritic length and fewer dendritic branch points on pyramidal CA1 and CA3 neurons, when compared with nulliparous or multiparous females [137]. The underlying mechanisms for the observed shrinkage are not well understood; however, it might be due to the lactation-associated hypercorticism, as studies in male rats revealed dendritic atrophy in hippocampal pyramidal cells after chronic stress-induced elevation of glucocorticoids [138]. Interestingly, not only the dendritic architecture seems to be modulated by motherhood, but typical neurogenic regions, like the hippocampus and the OB, also undergo remodeling of dendritic spines. Kopel et al. performed numerous elegant studies in mice revealing that dendritic spines of adult born granule cells (abGCs) underlie changes during lactation that might contribute to olfactory encoding during that time and thereby have a direct impact on mother-pup interactions. By injecting a lentivirus that encoded synaptophysin which was fused to GFP into the stem cell niche of the SVZ, they were not only able to show that abGCs of lactating females show an increase in the density of presynapses, but also that their spine stability and integration into the bulbar network are increased during motherhood [139]. Similar changes seem to occur in the hippocampus, where an increase in spine density on apical dendrites of CA1 pyramidal neurons and DG granule cells has been observed in late pregnant and postpartum rats [131,140]. While the increased spine density during pregnancy seems to be mediated by estrogen as suggested by studies in nulliparous rats that showed comparable changes after a hormone-stimulated pregnancy [141], such a regulation is unlikely during lactation as estrogen levels drop after parturition. Thus, it might be that pregnancy-generated spines either maintain throughout lactation or that environmental enrichment in form of maternal experience is beneficial for dendritic spine growth as seen before in humans [142] (and see [136] for review). Given the fact that dendritic arborization of these CA1 neurons has been shown to be diminished during lactation as mentioned before, the observed increase in dendritic spines might be seen as compensatory mechanism to increase the efficacy of synaptic inputs despite the reduced dendritic length and number of branching points in those dams. Definitely, the increase in spine density is only transient during lactation and dependent on the presence of pups, as there is a decline in primiparous rats after weaning [137]. However, the persistence of dendritic spines might also depend on the reproductive experience, as multiparous females have been shown to have more dendritic spines after weaning compared to primiparous mothers [137,143]. Given the fact that the CA1 region of the hippocampus is connected with the mPFC, the before mentioned peripartum-associated alterations in hippocampal spine density may also implicate changes in the forebrain. Indeed, dendritic architecture of the mPFC cortex undergoes an intense remodeling during lactation. By analyzing layers 2 and 3 pyramidal neurons of late lactating rats (LD20-24) Leuner and Gould were able to show that dendritic length and branching, as well as the number of spines on apical and basal dendrites, were increased, which directly coincided with an improved behavioral flexibility as assessed by an attentional set shifting task [57], a task which has been previously shown to depend on the PFC [144]. The onset of such dendritic changes in the mPFC seem to occur even earlier during lactation, as we made similar observations when we compared 3D reconstructions of neurons in the infralimbic mPFC of early lactating (LD3-5) and nulliparous rats (own unpublished results). These results are astonishing, given that the adverse hormonal environment during lactation and high glucocorticoid levels have been associated with a diminished dendritic architecture and spine density in the mPFC of nulliparous rats [145,146]. However, the effect of altered glucocorticoid levels during lactation might be highly region-specific as an increase in mushroom spines on apical and basal dendrites has been reported in lactating rats that have been chronically treated with high doses of CORT (40 mg/kg/day) between LD2 and LD23 [147]. Either way, it seems that the postpartum period or the associated increase in neuronal activity by the stimulatory effect of pup-contact [148,149] is able to buffer against the adverse basal hypercorticosterone environment and promotes dendritic growth in the mPFC. One hormone that might play a key role in this respect is OXT, which is known to be released during mother-infant contact in humans and rodents (for review see [150]) leading to an activation of the prelimbic prefrontal cortex [148]. We have previously shown that chronic stress during pregnancy in rats impairs the peripartum-associated increase in OXT activity in the PVN [151]. Given the fact that a reduction in the mean apical dendritic length in the mPFC has been associated with increased levels of anxiety in male rats [152] and chronic stress has been shown to increase anxiety during lactation [151], it would be interesting to see if dendritic morphology is a prerequisite of the peripartum-observed anxiolysis and if OXT might play a role in dendritic remodeling of the mPFC during that time.
As already discussed in one of the previous sections, the OXT system of the hypothalamus undergoes tremendous peripartum-associated changes with respect to synaptic plasticity. Aside from those changes in synaptic transmission, neuronal-glial remodeling, and rearrangement of synaptic inputs, morphologic changes of OXT neurons have been reported in lactating rodents. Dendritic trees of the magnocellular neurons of the SON undergo cytoarchitectonic reorganization during lactation that are very much evocative of those reported in the hippocampus as described before and can effectively contribute to lactation-induced neuronal plasticity by altering the electrical and integrative properties of OXT neurons during that time. In a number of elegant studies in rats Stern and Armstrong revealed that the dendritic trees of OXT neurons in the SON shrink during lactation. The reduction in total dendritic length was accompanied by a loss of dendritic branching on middle order branches (100-200 m from soma) of these neurons [153]. Given the broad consensus that an increased dendritic arborization and length usually leads to beneficial outcomes in behavior (i.e., reduced anxiety and increased cognitive performance) [57,152], these results might initially be surprising; however, the observed changes might contribute to the specific properties of the OXT system during lactation. Hence, a diminished branching would increase the postsynaptic space and thereby alter the electrical properties and the efficacy of synaptic inputs, which reach the soma to determine the output of the neuron [154]. This in fact might allow the high-frequency bursting activity pattern typically seen in OXT neurons during lactation leading to the milk ejection reflex [155]. Moreover, the dendritic shrinkage might involve a loss in synaptic contacts, leading to neurons that will deal with fewer, but more specific inputs in a more specific way. Indeed, the response of OXT neurons to nonspecific/not pup-related stimuli has been shown to be reduced during lactation [156,157].
We would also like to refer the reader to Table 1 that summarizes the findings discussed in this section.

Behavioral Adaptations during the Peripartum Period
During the peripartum period there is a whole set of cognitive and behavioral changes. Clearly, the display of maternal behavior, which also includes the expression of maternal aggression, is one key behavioral change that occurs during the peripartum period. As most mammalian species are not spontaneously maternal, the elevated availability of "maternal" neuropeptides like OXT [3,158], PRL [91,159], as well as AVP [95,160] and their respective receptors [39,42,90,94,161], is a prerequisite for the onset and maintenance of the complex repertoire of maternal behaviors. Peripartumassociated alterations in those brain factor systems not only are of vital importance for maternal behavior, but also they act in concert to decrease anxiety during lactation [37,44,91,116], as revealed by inhibition studies in rats [44,91,116,162] (and see [1] for review). The peripartumassociated anxiolysis, which can also be observed as increased calmness in breast-feeding mothers [25], is known to be accompanied by a coherent increase in aggression during lactation [163][164][165]. In this context, the CRH system, whose activity is decreased during lactation as discussed before, has been shown to be of relevance. Indeed, increasing CRH availability results in lower levels of maternal aggression in rodents [166,167]. Thus, it appears that the reduced activity of the CRH system not only is involved in the stresshyporesponsiveness during lactation, but also is an important regulator of maternal aggression and anxiety. It is likely that the peripartum-associated changes in anxiety and aggression not only are essential to ensure protection and survival of the offspring [168,169], but are also fundamental for maternal mental health. Aside from the above-mentioned changes in maternal behavior, that is, maternal anxiety and maternal aggression, the peripartum period is a time of alterations in cognitive abilities, particularly those that are associated with spatial learning, memory, and navigation as revealed in several animal and human studies using different tests to assess cognitive performance. The outcome, that is, an increase or decrement in cognitive abilities, seems to strongly depend on a variety of factors like species, parity, fetal sex, time of assessment, and test used (see [170,171] for review). Studies in rodents indicate that early and midpregnancy are generally associated with an increase in spatial working and reference memory in a couple of hippocampus-dependent tasks. Respectively, spatial memory is increased in dams as revealed by the Morris-Water-Maze [172][173][174] or Object-Placement-Task [175,176]. The observed improvements in cognitive ability seem to occur between PD7 and PD10, as pregnant rats have been shown to outperform nulliparous rats in the Morris-Water-Maze within this timeframe. However, the initial procognitive effects of early pregnancy are not continuous as they are followed by a decrement in cognitive abilities in late pregnancy into early lactation, a time when the mother's brain is usually referred to as the "pregnant brain. " Accordingly, human mothers show a significant decrease in working memory, verbal memory, word recall, visual memory, spatial memory, explicit and implicit memory, and attentional processes [177][178][179][180][181][182] and even self-rate them to have a poorer memory compared to the prepregnancy state [183]. The results from clinical research are in line with those from rodent studies, revealing a reduced performance in the Morris-Water-Maze on LD1 and LD4 [130]. Interestingly, deficits in memory are dependent on fetal sex, as human mothers being pregnant with a male fetus outperformed those pregnant with a female fetus in a working memory and spatial ability task [182]. Furthermore, an evolutionary component is likely to drive the observed changes in cognition, at least in part. Thus, while visual memory is diminished in human mothers, recognition memory is unaffected [184,185], indicating that the latter might be ethologically more important and therefore be maintained. After all, also the changes in late pregnancy and early lactation are only transient and give the way to cognitive improvements being effective throughout the late postpartum period and weaning and long into the process of aging even under conditions of stress. Respectively, late lactation has been associated with an increase in spatial working memory in a Land-Maze-Task [186], the Morris-Water-Maze [187], and Radial-Arm-Maze [188,189], as well as memory in an Object-Placement-Task in rodents [190]. In contrast to those tasks requiring spatial memory, tests assessing nonspatial memory did not reveal changes during the postpartum period in rodents [187], suggesting that the peripartum-associated changes in cognition play a predominant role in effective and efficient foraging, which is highly dependent on the spatial ability of the mother. Similar to the findings in rodents, human studies found verbal, semantic, and working memory, as well as attention, to be improved even two years after delivery [191]. Interestingly, maternal experience seems to have a long-term protective effect on learning, even under conditions of stress as revealed by studies in rats by Maeng and Shors [192]. They showed that exposure to an acute stressor suppressed learning in a classical eyeblink conditioning in virgins, but not in females that had been mothers five to nine month before the testing [192]. The peripartum-associated improvement in cognitive function seems to require structural reorganization of different brain regions like the hippocampus or the mPFC. Indeed, postpartum rats (LD20-LD24) showed an improved performance in an attentional set shifting task that was coincident with an increase in dendritic spines on pyramidal neurons of the mPFC [57]. The mentioned improvement was selective to extradimensional set shifting, which is known to require the mPFC, but not discrimination learning, intradimensional set shifting, or reversal learning [57]. Similarly, the observed increase in hippocampal-dependent learning during motherhood [188,189] might depend on dendritic remodeling in CA1 and CA3 regions of the hippocampus [137]. Given the well-known link between spatial learning/memory and hippocampal neurogenesis [193,194] and the fact that hippocampal neurogenesis is altered during the peripartum period as described before, it might be hypothesized that the observed alterations in cognition might be based on this relationship. Assessing hippocampal neurogenesis, spatial reference, and working memory in nulli-, primi-, and multiparous rats, Pawluski et al. nicely revealed that parity is an essential factor affecting both parameters. Nevertheless, it seems that neurogenesis might only play a minor role in peripartumassociated changes in cognition, since cell survival is diminished in primiparous rats across the postpartum period, while such animals outperform nulliparous control animals in cognition [132,188,189]. Other evidence from rodent and human studies suggests that parity might be an important factor influencing cognitive ability in age, although the outcome is controversial. In rodents, multiparity has been linked to better performance in several cognitive tasks when tested between six and 24 month of age that is long past their reproductive experience. Respectively, multiparous rats outperformed nulliparous and/or primiparous rats in a spatial memory task, object recognition/placement, and spatial memory task [195,196]. Importantly, multiparity and spatial learning/memory performance in the Morris-Water-Maze was negatively correlated with the level of immunoreactive amyloid precursor protein (APP), a marker of neurodegeneration and cognitive loss [195]. These results are in contrast to studies in humans indicating that the number of lifetime pregnancies is positively correlated with the risk to develop early onset Alzheimer's disease [197].

Molecular Processes That Underlie the Observed Peripartum Changes and Consequences of Malfunction
As illustrated throughout this review, there are numerous essential alterations in physiology, behavior, and neuroplasticity during pregnancy and lactation. Although, some of their underlying mechanisms have already been addressed in the respective section, in this chapter we would like to highlight some molecular mechanisms in more detail that significantly contribute to the peripartum-observed changes. Moreover, we will outline the consequences of malfunction in such systems for the pregnant or lactating mother. As discussed before, there are some striking adaptations of the hypothalamic OXT system during the peripartum period. Given the extent of those changes and the importance of the OXT system during that susceptible time, the question of their underlying molecular mechanisms is raised. One molecule that has been shown to be a key player in the peripartum-observed hypothalamo-neurohypophysial structural synaptic plasticity is PSA-NCAM. This neural cell adhesion molecule is abundantly expressed on astrocytic surfaces of rodent glial cells in the SON during the peripartum period [198,199] and is an important regulator of cell adhesion and contact-dependent cell surface interactions (for review see [200,201]). Indeed, PSA-NCAM is an essential prerequisite for the structural synaptic changes in the magnocellular nuclei of the hypothalamus, as revealed by enzymatic perturbation experiments. Thus, if endoneuraminidase was microinjected in the hypothalamic magnocellular nuclei in vivo and thereby enzymatically removing PSA from NCAM, neuronal, glial, and synaptic remodeling typically associated with lactation was inhibited [198]. Interestingly, OXT itself seems to stimulate the PSA-NCAM regulation of its own synaptic plasticity during the peripartum period, as ICV administration of OXT induces changes similar to those seen under physiological stimulation, that is, lactation, when OXT levels are high [202,203]. Hence, OXT which is dendritically released during parturition and lactation [43] may play a major role to induce synaptic plasticity in the hypothalamus during the peripartum period.
Although PSA-NCAM has some regulatory influence on SVZ/OB neurogenesis by stimulating tangential migration from the SVZ to the OB in rats and mice [204,205], the main regulatory factors for neuroplasticity in those regions are others. Shingo et al. were the first to elucidate that the endocrine state of an animal can actually influence the rate of neurogenesis. In more detail they were able to show that the rise in central PRL levels during pregnancy is the main driving factor for the increase in SVZ proliferation on PD7 and consequently the increased number of OB neurons two to four weeks later [115]. Indeed, lowering serum PRL levels by administration of bromocriptine during pregnancy led to a decrease in cell proliferation and cell survival in the SVZ/OB [115]. Moreover, they revealed that the action of PRL is mediated via PRL-R in the SVZ, as mice expressing the heterozygous form of the PRL-R (PRL + / − ) showed lower levels of cell proliferation on PD7 when compared with mice expressing the homozygous form of the receptor (PRL + / + ) [115]. Interestingly, hippocampal neurogenesis was unaffected by bromocriptine treatment [116], suggesting that maternal experience can have opposite effects on the same form of structural plasticity in two different brain regions via different distinct regulatory mechanisms. Although the factors regulating hippocampal neurogenesis during the peripartum period are still barley known, several studies significantly contribute to a better understanding of peripartumassociated changes in this neurogenic process. Thus, Rolls et al. nicely shed some light on one possible mechanism underlying the reduced hippocampal cell proliferation that they observed between PD11 and PD12 in mice [127]. Given the fact that the immune system has been shown to be an important regulator of neural plasticity and consequently learning and memory outside the context of reproduction with the general consensus that an increase in circulating cytokines has detrimental effects on neurogenesis, long-term potentiation, and remodeling of neural circuits, as well as learning and memory (see [206] for review), they hypothesized that their observations were due to the increase in cytokine levels during pregnancy as described above. Indeed, pregnant nude mice lacking the entire T-cell population did only show a modest decrease in cell proliferation. However, if those mice were reconstituted with T-cells the pregnancy-induced decrease in hippocampal neurogenesis was restored [127]. Importantly, the altered cytokine levels during pregnancy seem to have wide-ranging consequences on hippocampal integrity. Cipolla et al. have nicely shown that treatment of hippocampal slices with serum from pregnant rats for 48 h caused morphological changes in microglia characteristics of activation and neuroinflammation of hippocampal neurons [207]. These are interesting findings, especially with respect to the fact that preeclampsia is a state of immune system hyperactivity (for review see [208]) and human studies showing severe impairments in memory function after preeclampsia [209]. Although there are no animal models of preeclampsia assessing hippocampal neurogenesis or microglia to date, it would be interesting to see if memory impairments after preeclamptic pregnancies are due to cytokine-induced alterations in hippocampal neurons.
The mechanism of the lactation-associated decrease in hippocampal cell proliferation was nicely elucidated by the group around Leuner. In a number of elegant studies in rats they revealed that the main drive for the reduction in cell proliferation on LD2 and LD8 is the hypercorticism observed during that time, which is directly linked to the presence of pups. Accordingly, lowering CORT levels by either pup removal or adrenalectomy prevented the decrease in hippocampal cell proliferation [131]. Similarly, chronic injection of high CORT (40 mg/kg/day) during gestation or the postpartum period leads to a decrease in cell proliferation in rats when compared to an oil-treated control group [210]. These results are in contrast to our recent findings in rats where we were able to show that chronic stress during lactation reverses the lactation-associated decrease in cell proliferation despite a basal increase in CORT levels on LD6 [61]. These results may initially seem paradoxical, as stress or high CORT levels are commonly thought to impair adult hippocampal neurogenesis [210][211][212][213]. However, it has been shown that the DG proliferation rate may habituate to stress exposure and, thus, display a reduced sensitivity to HPA axis hormones [211]. Moreover, despite the literature supporting a link between a reduction in adult hippocampal neurogenesis and high basal CORT levels during stress and lactation, only a small percentage of precursor cells express CORT receptors [214].
Aside from the role of glucocorticoids in regulating peripartum-associated hippocampal neurogenesis there are a few other pregnancy/lactation hormones that might contribute to the observed changes. In fact, estradiol, which increases 50-fold throughout pregnancy and drops during late pregnancy has been shown to decrease cell proliferation during early lactation in a hormone-stimulated pregnancy regimen in rats [132]. To date there is no proof that OXT would affect hippocampal neurogenesis during the peripartum period. However, given the fact that OXT levels are high during that time and OXT has been shown to promote hippocampal neurogenesis in male rats even under conditions of chronic stress [215], a potential regulatory involvement of this neuropeptide seems to be feasible. Therefore, further studies are highly desired to elucidate mechanisms underlying peripartum-associated alterations in hippocampal neurogenesis, particularly given the clear link between hippocampal neurogenesis, stress-related mental illnesses, and antidepressant treatment [216][217][218][219][220], and with regard to the fact that the peripartum period is a time of high susceptibility for women to develop mood or anxiety disorders (see [1] for review). Interestingly, we recently showed that chronic stress during lactation severely affects different stages of hippocampal neurogenesis [61]. Aside from the above-mentioned increase in hippocampal cell proliferation chronic stress exclusively reduced the number of newly generated neurons without affecting the astrocytic niche. This is particularly striking given the fact that the addition of new neurons is thought to be of importance in relation to maternal bonding and care [221,222] and vice versa [126], which is severely affected not only in women with PPD [223], but also in rodents showing a change in maternal care and anxiety after chronic peripartum stress [151,164,224]. In this context, a dysregulation of the OXT system might be definitely taken into consideration as low plasma OXT concentrations during midpregnancy have been shown to significantly predict PPD symptoms two weeks postpartum in humans [225] and a reduced OXT mRNA expression in late pregnancy has been shown to be correlated with abnormal maternal behavior and anxiety in rodents [151].
Other factors that might contribute not only to peripartum-associated forms of neuroplasticity, but also with significant importance for proper placental development, are PlGF, VEGF, and their receptors, that are expressed in placental tissue and the brain (see [226,227] for review). Although there is increasing evidence for a parallelism between vessel and nerve patterning in humans and rodents [228][229][230], there is a lack of research assessing the role of PlGF and VEGF in various forms of peripartum-associated neuroplasticity. This is astonishing, given the importance of a sufficient availability of these angiogenic factors in proper placentation as seen in humans [227,231,232] and the fact that both proteins have already been shown to be positively involved in neurogenic processes outside the context of pregnancy or lactation. In more detail, PlGF promotes the survival of primary cortical neurons in an in vitro model of permanent middle cerebral artery occlusion [233]. Similarly, VEGF stimulates the expansion of neural stem cells in vitro [234] and has been shown to be essential for the increase in hippocampal blood vessel density, neurogenesis in the OB and DG, and a resulting antidepressant effect in rats [234,235]. Thus, it might be speculated that sufficient high levels of both PlGF and VEGF during the peripartum period are required to ensure neuroplasticity of the maternal brain and consequently maternal mental health. Although, to date there is no proof for an involvement of placental factors in the development of postpartum mood or anxiety disorders, it is clear that a malfunction in those systems mainly drives placental and endothelial dysfunction in preeclampsia. Characterized by the core symptoms hypertension and proteinuria after 20 weeks of gestation, preeclampsia is a leading cause of maternal and fetal morbidity and mortality [227]. Although there has been extensive research over the last decades revealing mechanisms underlying the pathology of preeclampsia, what is the cause and what is the consequence of the disease remain elusive. Nevertheless, one key mechanism seems to be based on a dysfunction of cytotrophoblasts, which, under normal conditions, acquire tumor-like properties that allow them to invade the uterus and promote vasculogenesis and subsequent angiogenesis by secreting VEGF and PlGF [236,237]. In preeclamptic patients unbound plasma levels of these angiogenic factors have been shown to be severely diminished due to an increased expression of soluble fms-like tyrosine kinase sFlt-1 [238], which diminishes the binding of VEGF and PlGF to their usual transmembrane receptor Flt-1. This in fact will start up a couple of processes that will result in abnormal placental perfusion and microvascular oxidative damage (for review see [227]) and the occurrence of the typical core symptoms. Indeed, lowering the elevated level of sFlt-1 in very preterm preeclamptic women by dextran sulfate apheresis effectively improved the outcome for mother and fetus [239]. As mentioned above, other symptoms of preeclampsia, aside from proteinuria and hypertension, are severe cognitive impairments of the mothers during lactation [209]. Although the observed memory impairments might be due to an increased CNS permeability during preeclamptic pregnancy [240], the fact that PlGF and VEGF act as neuroprotective and neurotrophic as discussed above raises the question about a reduced neuroplasticity in preeclamptic women that might underlie the observed impairments in cognition.

Conclusions
To date there has been extensive research revealing peripartum-associated adaptations on the physiological and behavioral level, as well as on several forms of neuronal plasticity of the maternal brain. As outlined throughout the review there have been numerous studies nicely revealing that changes in the OXT and PRL system, altered levels of angiogenic factors like PlGF and VEGF, as well as immunological parameters tremendously contribute to adaptations of the maternal physiology, the function of "maternal" brain regions, and the resultant behavioral repertoire. Moreover, it is known that malfunction of these systems can lead to peripartum-associated physiological and psychological pathologies such as preeclampsia or depression. Although, there is an incontrovertible parallelism between factors regulating vasculogenesis and angiogenesis during placentation as well as neuroplasticity, there is a lack of research perceiving the placenta and the brain, as well as their regulatory mechanisms rather as a peripartum entity than as isolated organs. Thus, as VEGF and PlGF might play bigger roles than currently anticipated and the possibility that the cytokine and immune status of women during and after pregnancy might be involved in the physiological and structural changes observed in these critical periods of life, future studies are highly desirable to answer those open questions.