Influence of Hsp90 and HDAC Inhibition and Tubulin Acetylation on Perinuclear Protein Aggregation in Human Retinal Pigment Epithelial Cells

Retinal pigment epithelial (RPE) cells are continually exposed to oxidative stress that contributes to protein misfolding, aggregation and functional abnormalities during aging. The protein aggregates formed at the cell periphery are delivered along the microtubulus network by dynein-dependent retrograde trafficking to a juxtanuclear location. We demonstrate that Hsp90 inhibition by geldanamycin can effectively suppress proteasome inhibitor, MG-132-induced protein aggregation in a way that is independent of HDAC inhibition or the tubulin acetylation levels in ARPE-19 cells. However, the tubulin acetylation and polymerization state affects the localization of the proteasome-inhibitor-induced aggregation. These findings open new perspectives for understanding the pathogenesis of protein aggregation in retinal cells and can be useful for the development of therapeutic treatments to prevent retinal cell deterioration.


Introduction
Retinal pigment epithelial (RPE) cells are exposed to chronic oxidative stress. They must constantly absorb light energy and phagocyte lipid rich photoreceptor outer segment shed from neural retina due to normal physiological visual cycle. Oxidative stress refers to progressive cellular damage that contributes to protein misfolding and functional abnormalities in the RPE cells during cellular senescence [1]. The accumulation of this damage in the postmitotic RPE cells seems to be one of the key events in the development of agerelated macular degeneration (AMD), the leading cause of blindness in the elderly in the developed countries. The RPE cells ensure the survival of neural cells, rod, and cones. In senescent RPE cells, this ability is reduced causing secondary adverse effects on the neural retina, ultimately leading to loss of vision. Both intra-and extracellular aggregation processes are crucial in cell degeneration and AMD [2].
Efficient removal of misfolded proteins from cytoplasm is critical for cellular survival and adaptation. However, potentially toxic misfolded protein aggregates accumulate during the aging process [3,4]. Control of protein turnover is particularly important in postmitotic cells, since the accumulation of malfunctioning proteins may be highly detrimental to the cells [5]. Once heat shock proteinmediated protein folding fails, the misfolded proteins are usually tagged with a ubiquitin (Ub) moiety that directs the complex to the ubiquitin/proteasomal protein degradation pathway (UPP) [6]. It is believed that the aggregation of oxidized and ubiquinated proteins is due to a decline in the proteasomal activity with age, and that this also occurs in RPE cells [7][8][9][10]. Protein aggregates formed in the cell periphery are delivered along microtubulus network by dynein-dependent retrograde trafficking to a juxtanuclear location where they form aggresomes [11][12][13]. The development of these aggresomes is part of a cellular defence mechanism against misfolded proteins [14], and it can be inhibited by drugs that depolymerize microtubules [15,16].
Tubulin undergoes various posttranslational modifications including polyglutamylation, polyglycylation, carboxyterminal cleavage, and acetylation [17,18]. Acetylation is unique among the known tubulin modifications, in that it occurs on the lysine 40 of α-tubulin which can be found on stable microtubules in most cell types [19]. The deacetylation of the lysine residue in tubulin is catalyzed by enzymes called histone deacetylases (HDACs). In contrast, histone acetyltransferases (HATs) transfer acetyl groups to lysine residue to increase acetylation of tubulin [20]. This results in a balance between acetylation and deacetylation states of tubulin, and any shift in this balance results in changes in the regulation of tubulin function [21]. The HDAC6 is the major cytoplasmic tubulin deacetylase [21][22][23]. Moreover, it efficiently binds mono-and polyubiquitin molecules [24][25][26]. One interesting role of tubulin and tubulin modifying deacetylases is their influence on aggregation of misfolded proteins [12]. HDAC6 contributes to the degradation of aggregated proteins because it is able to bind to both polyubiquitinated and dynein motor proteins as an adaptor protein to help transport misfolded proteins along microtubules into aggresomes which are finally degraded by autophagy [13,22,[27][28][29]. HDAC6 is able to sense ubiquitinated cellular aggregates and consequently induces the expression of major cellular chaperones by triggering the dissociation of a repressive HDAC6/HSF1 (heat-shock factor 1)/HSP90 (heat-shock protein 90) complex and subsequent HSF1 activation. HDAC6 therefore appears as a master regulator of the cell protective response to cytotoxic protein aggregate formation [13].
We have recently shown that proteasome inhibition evoked perinuclear protein accumulation which then leads to the autophagy-mediated removal of the deposits [29]. In the present study, we demonstrate that Hsp90 inhibition with geldanamycin can effectively decrease the proteasome inhibitor-induced aggregation. Increased tubulin acetylation was observed in response to Hsp90, proteasome, and HDAC inhibition, but the acetylation level was not correlated with the amount of the aggregates in the ARPE-19 cells. However, the tubulin polymerization state did influence the localization of the aggregates.

Phase Contrast Microscopy.
Phase contrast microscopy photographs were taken from live cells with a Nikon Eclipse TE300 (Nikon, Tokyo, Japan) microscope.

Electron Microscopy.
For transmission electron microscopy (TEM), the cell culture samples were prefixed in 2.5% glutaraldehyde (in 0.1 M phosphate buffer, pH 7.4) for 2 hrs at room temperature, followed by washing (15 min in 0.1 M phosphate buffer). The samples were postfixed in 1% osmium tetraoxide (in 0.1 M phosphate buffer) for 1 hr at room temperature and washed as before prior to standard ethanol dehydration. Subsequently, the samples were infiltrated and embedded in LX-112 resin (Ladd Research Industries, Burlington, VT, USA). Polymerization was carried out at 37 • C for 24 hrs and at 60 • C for 48 hrs. The sections were examined in a JEOL-1200EX transmission electron microscope (Jeol, Tokyo, Japan) at 80 kV.

Hsps and Protein
Aggregation. The ARPE-19 cells were either nonstressed, or exposed to drugs, that is, 5 μM MG-132 or 0.25 μM geldanamycin, 1 μM trichostatin A or 1 μM taxol or 5 μM nocodazole for 24 hours. In addition, the cells were treated simultaneously with MG-132 in conjunction with geldanamycin, trichosatin A, taxol or nocodazole up to 24 hours. Only a single MG-132 insult or combination treatment with taxol induced a typical perinuclear aggregation in ARPE cells (Figures 1(a) and 2(a)). Interestingly, Hsp90 inhibition with geldanamycin effectively suppressed MG-132 induced protein aggregation. HDAC inhibition with trichostatin A or tubulin depolymerization with nocodazole evoked dispersed the mid-peripherical aggregation process during proteasome inhibition. With all of the insults, cytoplasm underwent a similar effective aggregation clearance when exposed to MG-132 for 24 hours and then allowed to recover for 24 hours in normal cell culture medium (Figures  1(b) and 2(b)). A robust elevation of Hsp70 protein expression was seen in response to MG-132 or geldanamycin exposures ( Figure 3). Hsp90 levels were not markedly affected by the treatments. Abrogation of proteasome-mediated protein degradation caused a clear increase in the amount of Hsp70 which has a cytoprotective capacity in the MG-132-treated ARPE-19 cells [29]. The classical transcriptional heat-shock gene induction is attributable to the activation of HSF1 transcription factor [32]. In the activation process Hsp90 and Hsp70 dissociate from HSF1 transcription factor [33,34]. Geldanamycin has been shown to bind Hsp90, to inhibit its function and to elicit the Hsp90 client protein degradation in proteasomes [35,36]. In line with previous studies [37][38][39], the geldanamycin was found to trigger a strong expression of Hsp70, while the Hsp90 response remained weaker. The response is likely mediated through HSF1 transcriptional activation [33,34]. The increase in the amount of inducible Hsp70 might be one of the regulators suppressing the proteasome inhibitor-induced aggregation process [29], when Hsp90 is simultaneously inhibited. It has also been documented that a Hsp90 inhibitor may prevent the aggregation of protein by regulating client protein posttranslational modifications [40]. Since Hsp90 inhibition has been reported to trigger autophagy clearance [41,42], we wished to analyze the autophagy induction marker LC3 I/II levels in ARPE cells treated with geldanamycin or MG-132 solely or both together for 6, 12, and 24 hours. Our findings clearly show that proteasome inhibition mildly induced autophagy, when related to LC3 II levels, but this was not involved in geldanamycin treatments (Figure 4). This indicates that autophagy clearance is not implicated in the suppression of protein aggregation during Hsp90 and proteasome inhibition in the ARPE-19 cells.

3.2.
Ubiquitination. Cellular ubiquitin-protein (Ub) conjugate levels were analyzed by western blotting from cells treated as described above. The level of Ub conjugation was not changed by geldanamycin, trichostatin A, taxol, or nocodazole treatment, but a very intensive induction of  GA  TSA  TAX  NOC  GA  TSA  TAX  NOC  Med  GA  TSA  TAX  NOC   24   Journal of Biomedicine and Biotechnology ubiquitination was seen in response to MG-132 exposure whether geldanamycin, trichostatin A, taxol, or nocodazole were present or not ( Figure 3). Inhibition of Hsp90 therefore seems to have only a minor impact on the level of ubiquitination in the ARPE-19 cells, although Hsp90 has been shown to regulate both proteasomal and autophagy clearance through its client proteins [41][42][43]. The ubiquitin levels were slightly reduced when proteasome inhibition was removed and the cells recovered either with geldanamycin, trichostatin A, taxol, or nocodazole compared to incubations of MG-132 together with these chemicals. This is apparently explained by the autophagic clearance of proteasome-inhibitor-induced aggregates, as we have recently documented [29].

Acetylation.
Protein aggregates formed at the cell periphery are delivered along the microtubulus network by dynein-dependent retrograde trafficking to a juxtanuclear location where they form aggresomes [11,12]. The reversible acetylation of α-tubulin has been linked to the regulation of microtubule stability and function [21]. Decreased tubulin acetylation has been shown in some reports to reduce microtubule stability [21,22], but in other experiments no association was observed [44,45]. HDAC6 deacetylates tubulin, and Hsp90, and forms complexes with many other proteins, including ubiquitinated proteins. HDAC6 also interacts with a component in the dynein-dynactin microtubule motor complex and regulates protein aggregation trafficking [13,45,46]. Trichostatin A is a classical inhibitor of HDAC deacetylase activity and thus it also blocks the HDAC6 [47]. The phase contrast and transmission electron microscopic analysis revealed dispersed cytoplasmic aggregation in response to simultaneous treatment of trischostatin A or nocodazole and proteasome inhibition (Figures 1 and 2). Trichostatin A evoked a clear increase in tubulin acetylation levels, while in the situation of tubulin depolymerizator induced by nocodazole, tubulin acetylation remained at control level ( Figure 3). In contrast, a tubulin stabilizer, taxol induced tubulin acetylation that was not, however, as extensive as that evoked by trichostatin A. Hsp90 and proteasome inhibition evoked a similar slight increase in the tubulin acetylation levels. All these findings indicate that total tubulin acetylation is not related to perinuclear aggregation. The HDAC inhibition by trichostatin A probably affects the dynein motor and regulates aggregation localization, but does not prevent the formation of aggregates. Moreover, the polymerization state of tubulin regulates the localization of proteasome inhibitor-induced aggregates, but this process cannot be estimated by measuring total tubulin acetylation levels. Hsp90 inhibition seems to prevent aggregation rather than regulating trafficking through the tubulin network.

Conclusions
Hsp90 inhibition is effectively involved in the regulation of protein aggregation that is independent of HDAC inhibition or tubulin acetylation levels in the RPE cells. These findings open new perspectives for understanding the pathogenesis of protein aggregation in retinal cells, and they may be useful in the development of therapeutic treatments to prevent retinal cell deterioration, that is, during aging.