Analysis of chorion proteins of Bombyx mandarina in comparison with those of Bombyx mori

BUNGO SAKAGUCHI; KAZUNORI SUGAHARA; OSAMU NINAKI; MARIAN R. GOLDSMITH; YUTAKA KAWAGUCHI; KATSUMI KOGA; SI KAB NHO

doi:10.11416/kontyushigen1930.67.197

Analysis of chorion proteins of Bombyx mandarina in comparison with those of Bombyx mori

BUNGO SAKAGUCHI, KAZUNORI SUGAHARA, OSAMU NINAKI, MARIAN R. GOLDSMITH, YUTAKA KAWAGUCHI, KATSUMI KOGA, SI KAB NHO

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Keywords: B. mandarina, B. mori, crossing experiments, chorion proteins, localization of Hc proteins

JOURNAL FREE ACCESS

1998 Volume 67 Issue 3 Pages 197-203

DOI https://doi.org/10.11416/kontyushigen1930.67.197

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Abstract

The proteins of the eggshell or chorion of Bombyx mori, its putative ancestor Bombyx mandarina, and the offspring after crossing of these species were analyzed by two-dimensional gel electrophoresis. Some of the high cysteine (Hc) proteins were found to be specific to B. mandarina. This feature was co-dominantly inherited in the F₁. The overall traits of chorion proteins in BF₁ were found to segregate with those of the larval body color, indicating that, like B. mori, the chorion genes are also linked to the body color (p) locus in B. mandarina. The previously known structure of chorion peculiar to B. mandarina (with a large air space under the outer layer) facilitated the artificial scraping off of the thin outermost layer. The protein analysis of this material clearly indicated the localization of the Hc proteins to the outer layer of the chorion.

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