This report describs our current attempts to design and test liver cell perfusion. Seven adult dogs were used in our experiment, they were divided in two groups at two and five respectively. One was control group, another was supported with hepatocyte group. Hepatic support system chamber was made of acryl board (10×10×1cm), and it was separated in three spaces with Neucljeopore Membrane Filter (NMF). Middle space was filled with hepatocyte (50-70g) of rabbit. This hepatocyte was isolated with #24 metal mesh from the liver which was wash out Lactete-Ringer's solution.
The chamber was connected with hepatic failure canine external shunt. The longest survival of the dog was 18 hours. This results were suggested that chamber was effective to the theraphy of hepatic failure. But the chamber membrane area was not enough to big dogs.