You are here

Article Text

Article menu
Download PDFPDF
Small intestine
Fructose-1,6-biphosphate in rat intestinal preconditioning: involvement of nitric oxide
  1. A Sola,
  2. J Roselló-Catafau,
  3. E Gelpí,
  4. G Hotter
  1. Department of Medical Bioanalysis, Instituto de Investigaciones Biomédicas de Barcelona (IIBB-CSIC-IDIBAPS), C/Roselló 161, 7ª planta, Barcelona, Spain
  1. Dr G Hotter, IIBB-CSIC-IDIBAPS, Department of Medical Bioanalysis, C/Roselló, 161, 7ª planta, 08036 Barcelona, Spain.ghcbam{at}iibb.csic.es

Abstract

BACKGROUND AND AIMS Inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by nitric oxide (NO) in intestinal preconditioning could modify the rate of formation of glycolytic intermediates. Fructose-1,6-biphosphate (F16BP) is a glycolytic intermediate that protects tissue from ischaemia/reperfusion injury. We evaluated if F16BP may be endogenously accumulated as a consequence of GAPDH inhibition by NO during intestinal preconditioning in rats.

METHODS We assessed: (1) effect of preconditioning on F16BP content; (2) effect of NO on GAPDH activity before and during sustained ischaemia; and (3) protective effect of F16BP in control, ischaemic, and preconditioned animals with or without administration ofN-nitro-l-arginine methyl ester (l-NAME), NO donor, or F16BP.

RESULTS Preconditioned rats showed a significant transient decrease in GAPDH activity and also maintained basal F16BP levels longer than ischaemic rats.l-NAME administration to preconditioned rats reversed these effects. F16BP administration to ischaemic rats decreased protein release in the perfusate. Administration of F16BP to l-NAME treated rats attenuated the harmful effect of l-NAME.

CONCLUSIONS Our study indicates that F16BP may be endogenously accumulated in preconditioned rats as a consequence of inhibition of GAPDH by NO, and this may contribute to the protection observed in intestinal preconditioning.

  • fructose-1,6-biphosphate
  • glyceraldehyde- 3-phosphate dehydrogenase
  • intestinal preconditioning
  • ischaemia/reperfusion injury
  • nitric oxide

  • Abbreviations used in this paper

    F16BP
    fructose-1,6-biphosphate
    GAPDH
    glyceraldehyde-3-phosphate dehydrogenase
    iNOS
    inducible nitric oxide synthase
    I/R
    ischaemia/ reperfusion
    l-NAME
    N-nitro-l-arginine methyl ester
    NO
    nitric oxide
    NONOS
    spermine NONOate
    1400W
    N-(3-(aminomethyl)benzyl) aceramidine, dihydrochloride

    • https://doi.org/10.1136/gut.48.2.168

    Statistics from Altmetric.com

      Request Permissions

      If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

    • Abbreviations used in this paper

      F16BP
      fructose-1,6-biphosphate
      GAPDH
      glyceraldehyde-3-phosphate dehydrogenase
      iNOS
      inducible nitric oxide synthase
      I/R
      ischaemia/ reperfusion
      l-NAME
      N-nitro-l-arginine methyl ester
      NO
      nitric oxide
      NONOS
      spermine NONOate
      1400W
      N-(3-(aminomethyl)benzyl) aceramidine, dihydrochloride
      • View Full Text