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Development of a Quantitative Enzyme Immunoassay of Brain-Derived Neurotrophic Factor on the Basis of a Recombinant Antigen

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Abstract—One of the pathophysiological mechanisms of the development of some mental diseases, including depressive disorders, is a decrease in the expression of brain-derived neurotrophic factor (BDNF), mainly in the limbic region and prefrontal cortex of the brain. Immunochemical screening of BDNF in biological fluids showed that the levels of this protein can be considered as a marker of predisposition to depression and a prognostic marker of the effectiveness of therapy. The use of modern technologies for the production of recombinant proteins makes it possible to develop highly standardized ELISA systems for the determination of this antigen in biological fluids. This paper describes a method for creating a test system for quantitative ELISA of brain-derived neurotrophic factor on the basis of recombinant protein BDNF and antibodies obtained as a result of immunization with recombinant BDNF.

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Authors and Affiliations

  1. Serbskii National Medical Research Center for Psychiatry and Narcology, Ministry of Health of Russia, Moscow, Russia

    O. V. Pavlova, N. V. Andryushchenko, O. I. Gurina & K. A. Pavlov

  2. Moscow Research Institute of Psychiatry, Branch of the Serbskii National Medical Research Center for Psychiatry and Narcology, Ministry of Health of Russia, Moscow, Russia

    A. A. Murashko

Authors
  1. O. V. Pavlova
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  2. A. A. Murashko
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  3. N. V. Andryushchenko
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  4. O. I. Gurina
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  5. K. A. Pavlov
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Corresponding author

Correspondence to O. V. Pavlova.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Ethical approval. All applicable international, national and/or institutional guidelines for the care and use of animals have been followed. All procedures performed in human research comply with the ethical standards of the institutional and/or national research ethics committee and the 1964 Declaration of Helsinki and its subsequent amendments or comparable ethical standards.

Informed consent. Informed consent was obtained from each of the participants included in the study.

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Corresponding author; address: per. Kropotkinsky, 23, Moscow, 119034 Russia; e-mail: uovnew@mail.ru.

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Pavlova, O.V., Murashko, A.A., Andryushchenko, N.V. et al. Development of a Quantitative Enzyme Immunoassay of Brain-Derived Neurotrophic Factor on the Basis of a Recombinant Antigen. Neurochem. J. 17, 132–137 (2023). https://doi.org/10.1134/S1819712423010154

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  • DOI: https://doi.org/10.1134/S1819712423010154

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