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Staining of Living Monoaminergic Neurons with 4-[4-(Dimethylamino) Styryl]-N-Methylpyridinium Iodide as a Tool for Studying Their Topography and Functions

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Abstract—Analysis of the topography and functional activity of central and peripheral monoaminergic neurons is one of the priorities of neurosciences, since these neurons play a key role in the regulation of the most important functions of the brain and internal organs. After the development of methods for detecting monoaminergic neurons in the 1960s–1980s (histo-fluorescence, immunocytochemistry), numerous studies were performed to map monoaminergic neurons and describe their phenotype. Fundamentally new opportunities for studying monoaminergic neurons have appeared in the last ten years following the synthesis of a fluorescent ligand of membrane transporters of monoamines—4-[4-(dimethylamino) styryl)-N-methylpyridinium iodide (ASP+), which allows staining of living monoaminergic neurons. Our work aimed to expand the spectrum of ASP+ use in the study of central and peripheral monoaminergic neurons. When doing this, (1) we developed a method for ASP+ staining of living dopaminergic neurons in a primary culture of the mouse embryonic mesencephalon, which can be used for testing antiparkinsonian drugs in cell models of Parkinson’s disease; (2) using ASP+, we developed a method for assessing the permeability of the cerebrospinal fluid-brain barrier for monoamines and for mapping the monoaminergic neurons involved in the exchange of monoamines between the cerebrospinal fluid and the brain; (3) we showed that the systemic administration of ASP+ allows in vivo mapping of peripheral monoaminergic neurons and cells of peripheral organs expressing monoamine transporters; and (4) we showed that ASP+ cannot be used to assess the formation of the blood-brain barrier during ontogeny.

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ACKNOWLEDGMENTS

The work was performed using the equipment of the Collective Use Center of the Koltsov Institute of Developmental Biology of the Russian Academy of Sciences.

Funding

The study was financed by the Russian Science Foundation (project no. 20-14-00325).

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Authors and Affiliations

  1. Laboratory of Neural and Neuroendocrine Regulations, Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    V. E. Blokhin, T. S. Pronina, S. A. Surkov, A. R. Murtazina & M. V. Ugryumov

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  1. V. E. Blokhin
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  2. T. S. Pronina
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  3. S. A. Surkov
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  4. A. R. Murtazina
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  5. M. V. Ugryumov
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Corresponding author

Correspondence to M. V. Ugryumov.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Ethical approval. All manipulations with animals were performed in accordance with national and international requirements and rules approved by the Animal Welfare Committee of the Koltsov Institute of Developmental Biology of the Russian Academy of Sciences (mice, protocol No. 39 dated 09/10/2020; rats, protocol No. 44 dated 12/24/2020).

Additional information

M. V. Ugryumov is a corresponding author; address: ul. Vavilova 26, Moscow, 119334 Russia; phone: +7(499)135-8842; e-mail: michael.ugrumov@mail.ru.

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Blokhin, V.E., Pronina, T.S., Surkov, S.A. et al. Staining of Living Monoaminergic Neurons with 4-[4-(Dimethylamino) Styryl]-N-Methylpyridinium Iodide as a Tool for Studying Their Topography and Functions. Neurochem. J. 16, 155–160 (2022). https://doi.org/10.1134/S1819712422020040

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