Skip to main content
SpringerLink
Log in

A Comparative Pharmacognostic Study of Lowland Wood-Grass Peats of Different Origin for Substantiation of Their Use as Promising Sources of Biologically Active Humic Acids

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

A comparative pharmacognostic study of two lowland wood-grass peats of different origin from the Tomsk oblast deposits and of the physicochemical parameters of the structure of humic acids (HAs) isolated therefrom was carried out in order to substantiate the interchangeability of peat raw materials classified into the same peat type and to select a promising source of biologically active HAs for development of new drug candidates with non-specific biological activity. It was found that two lowland wood-grass peats of different origin from the Tomsk oblast deposits (Klyukvennoe and Tagan peatlands) have similar botanical compositions and pharmacognostic parameters. Humic acids were isolated from the peats by alkaline extraction and, according to a combination of physicochemical methods (UV and IR spectroscopy, elemental CHNS analysis, 13C-NMR spectroscopy, titrimetry), have common structural features due to the presence of the aromatic structure substituted with aliphatic radicals and of the polyconjugated system, identical aromaticity degrees, the predominance of hydrophilic over hydrophobic moieties, polydispersity, and pronounced reducing properties. This indicates the interchangeability of the peat raw materials, provided that the technology for HAs extracting from the peat is observed. The revealed distinctions between the HA samples, mainly in terms of the nitrogen content and the content of phenolic and quinoid moieties, the degree of condensation of aromatic structures, and the contents of lignin methoxy group and of carbohydrate moieties, made it possible to conclude that the HAs extracted from the Tagan deposit peat are more promising raw materials for the development of safe plant-based medicines effective in the prevention and treatment of a wide range of pathologies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.

Similar content being viewed by others

DATA AVAILABILITY

The data that support the findings of this study are available from the corresponding author upon reasonable request.

REFERENCES

  1. Orlov, D.S., Soil Humic Acids and the General Theory of Humification, Moscow: Mosk. Gos. Univ., 1990.

  2. Zykova, M.V., Logvinova, L.A., and Belousov, M.V., Tradits. Med., 2018, no. 2 (53), pp. 27–38.

    Google Scholar 

  3. van Rensburg, C.E., Phytother. Res., 2015, vol. 29, pp. 791–795. https://doi.org/10.1002/ptr.5319

    Article  CAS  PubMed  Google Scholar 

  4. Zykova, M.V., Schepetkin, I.A., Belousov, M.V., Krivoshchekov, S.V., Logvinova, L.A., Bratishko, K.A., Romanenko, S.V., Yusubov, M.S., and Quinn, M.T., Molecules, 2018, vol. 23, no. 4, p. 753. https://doi.org/10.3390/molecules23040753

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Zykova, M.V., Logvinova, L.A., Krivoshchekov, S.V., Voronova, O.A., Lasukova, T.V., Bratishko, K.A., Zholobova, G.A., Golubina, O.A., Perederina, I.A., Drygunova, L.A., Tveryakova, E.N., and Belousov, M.V., Khim. Rast. Syr’ya, 2018, no. 3, pp. 239–250. https://doi.org/10.14258/jcprm.2018033925

    Article  CAS  Google Scholar 

  6. Zykova, M.V., Belousov, M.V., Logvinova, L.A., Lasukova, T.V., Gorbunov, A.S., and Dygai, A.M., Bull. Exp. Biol. Med., 2017, vol. 163, no. 2, pp. 206–209. https://doi.org/10.1007/s10517-017-3767-1

    Article  CAS  PubMed  Google Scholar 

  7. Lasukova, T.V., Zykova, M.V., Belousov, M.V., Gorbunov, A.S., Logvinova, L.A., and Dygai, A.M., Bull. Exp. Biol. Med., 2019, vol. 166, no. 5, pp. 598–601. https://doi.org/10.1007/s10517-019-04399-y

    Article  CAS  PubMed  Google Scholar 

  8. Bratishko, K.A., Zykova, M.V., Ivanov, V.V., Buiko, E.E., Drygunova, L.A., Perminova, I.V., and Belousov, M.V., Khim. Rast. Syr’ya, 2021, no. 1, pp. 287–298. https://doi.org/10.14258/jcprm.2021018784

    Article  CAS  Google Scholar 

  9. Buiko, E.E., Zykova, M.V., Ivanov, V.V., Bratishko, K.A., Ufandeev, A.A., Grigor’eva, I.O., Tsupko, A.V., Mikhalev, D.A., Perminova, I.V., and Belousov, M.V., Razrab. Registr. Lek. Sredstv, 2021, vol. 10, no. 4, pp. 46–53. https://doi.org/10.33380/2305-2066-2021-10-4-46-53

    Article  CAS  Google Scholar 

  10. Belousov, M.V., Akhmedzhanov, R.R., Zykova, M.V., Gur’ev, A.M., and Yusubov, M.S., Pharm. Chem. J., 2014, vol. 48, no. 4, pp. 249–252. https://doi.org/10.1007/s11094-014-1088-5

    Article  CAS  Google Scholar 

  11. Zamoshchina, T.A., Gostyukhina, A.A., Zaitsev, K.V., Abdulkina, N.G., Zykova, M.V., Logvinova, L.A., Svetlik, M.V., Kurtsevich, E.A., Belousov, M.V., Lasukova, T.V., and Dygai, A.M., Bull. Exp. Biol. Med., 2020, vol. 169, no. 6, pp. 734–737. https://doi.org/10.1007/s10517-020-04967-7

    Article  CAS  PubMed  Google Scholar 

  12. Trofimova, E.S., Zykova, M.V., Ligacheva, A.A., Sherstoboev, E.Yu., Zhdanov, V.V., Belousov, M.V., Yusubov, M.S., Krivoshchekov, S.V., Danilets, M.G., and Dygai, A.M., Bull. Exp. Biol. Med., 2016, vol. 161, no. 5, pp. 687–692. https://doi.org/10.1007/s10517-016-3486-z

    Article  CAS  PubMed  Google Scholar 

  13. Trofimova, E.S., Zykova, M.V., Ligacheva, A.A., Sherstoboev, E.Yu., Zhdanov, V.V., Belousov, M.V., Yusubov, M.S., Krivoshchekov, S.V., Danilets, M.G., and Dygai, A.M., Bull. Exp. Biol. Med., 2016, vol. 162, no. 6, pp. 741–745. https://doi.org/10.1007/s10517-017-3702-5

    Article  CAS  Google Scholar 

  14. Trofimova, E.S., Zykova, M.V., Ligacheva, A.A., Danilets, M.G., Sherstoboev, E.Yu., Logvinova, L.A., and Belousov, M.V., Byull. Eksp. Biol. Med., 2021, vol. 172, no. 9, pp. 313–317. https://doi.org/10.47056/0365-9615-2021-172-9-313-317

    Article  Google Scholar 

  15. Zykova, M.V., Veretennikova, E.E., Logvinova, L.A., Romanenko, S.V., Bratishko, K.A., Belousov, M.V., Brazovsky, K.S., Yusubov, M.S., Lyapkov, A.A., Danilets, M.G., Trofimova, E.S., and Ligacheva, A.A., Environ. Res., 2020, vol. 191, p. 109999. https://doi.org/10.1016/j.envres.2020.109999

    Article  CAS  PubMed  Google Scholar 

  16. Gostishcheva, M.V., Belousov, M.V., Ismatova, R.R., Vasil’ev, K.Yu., Dmitruk, S.E., and Yusubov, M.S., Bashkir. Khim. Zh., 2009, vol. 15, no. 2, pp. 67–71.

    Google Scholar 

  17. Arkhipov, V.S. and Maslov, V.G., Khim. Rast. Syr’ya, 1998, no. 4, pp. 9–16.

    Google Scholar 

  18. Zykova, M.V., Danilets, M.G., Krivoshchekov, S.V., Trofimova, E.S., Likhacheva, A.A., Sherstoboev, E.Yu., Belousov, M.V., and Yusubov, M.S., RF Patent 2610446, 2017.

  19. GOST (State Standard) 28245-89: Peat. Methods for Determining the Botanical Composition and Degree of Decomposition, Moscow, 2006.

  20. State Pharmacopoeia of the Russian Federation, 14th Ed., Moscow, 2018. http://www.femb.ru/feml

  21. Ikeya, K. and Watanabe, A., Anal. Bioanal. Chem., 2015, vol. 402 (2), pp. 651–655. https://doi.org/10.1007/s00216-015-9127-y

    Article  CAS  Google Scholar 

  22. Orlov, D.S., Humic Substances in the Biosphere, Moscow: Nauka, 1993.

  23. Shiping, W., Meng, W., Guilong, L., Ming, L., Chunyu, J., and Zhongpei, L., J. Agric. Food Chem., 2018, vol. 66, no. 28, pp. 7514–7521. https://doi.org/10.1021/acs.jafc.8b01931

    Article  CAS  Google Scholar 

  24. Maryganova, V.V., Bambalov, N.N., Strigutskii, V.P., and Parmon, S.V., Khim. Tverd. Topl., 2013, no. 3, pp. 19–30. https://doi.org/10.7868/S0023117713030055

    Article  Google Scholar 

  25. Lishtvan, I.I., Kaputskii, F.N., Yanuta, Yu.G., Abramets, A.M., Monich, G.S., Navosha, Yu.Yu., Strigutskii, V.P., Glukhova, N.S., and Aleinikova, V.N., Vestn. Belorus. Gos. Univ., Ser. 2: Khim. Biol. Geogr., 2012, no. 1, pp. 18–23.

    Google Scholar 

  26. Nesterova, O.V. and Semal’, V.A., Vestn. Krasnoyarsk. Gos. Agrar. Univ., 2009, no. 10, pp. 29–35.

    Google Scholar 

  27. Lishtvan, I.I., Yanuta, Yu.G., Abramets, A.M., Navosha, Yu.Yu., Strigutskii, V.P., and Kaputskii, F.N., Khim. Tverd. Topl., 2006, no. 4, pp. 3–11.

    Google Scholar 

  28. Zaccone, C., Miano, T.M., and Shotyk, W., Org. Geochem., 2007, vol. 38, no. 1, pp. 151–160. https://doi.org/10.1016/j.orggeochem.2006.06.023

    Article  CAS  Google Scholar 

  29. Zherebker, A., Kostyukevich, Y., Kononikhin, A., Kharybin, O., and Konstantinov, A.I., Anal. Bioanal. Chem., 2017, vol. 409, no. 9, pp. 2477–2488. https://doi.org/10.1007/s00216-017-0197-x

    Article  CAS  PubMed  Google Scholar 

  30. Shin, H.S., Monsallier, J.-M., and Choppin, G.R., Talanta, 1999, vol. 50, no. 3, pp. 641–647. https://doi.org/10.1016/S0039-9140(99)00161-7

    Article  CAS  PubMed  Google Scholar 

  31. Kholodov, V.A., Konstantinov, A.I., Kudryavtsev, A.V., and Perminova, I.V., Pochvovedenie, 2011, no. 9, pp. 1064–1073.

    Google Scholar 

  32. Hertkorn, N., Permin, A.B., Perminova, I.V., Kovalevskii, D.V., Yudov, M.V., and Kettrup, A., J. Environ. Qual., 2002, vol. 31, no. 2, pp. 375–387. https://doi.org/10.2134/jeq2002.3750

    Article  CAS  PubMed  Google Scholar 

  33. Tadini, A.M., Pantano, G., Toffoli, A.L., Fontaine, B., Spaccini, R., Piccolo, A., Moreira, A.B., and Bisinoti, M.C., Sci. Tot. Environ., 2015, vols. 506–507, pp. 234–240. https://doi.org/10.1016/j.scitotenv.2014.11.012

  34. Amir, S., Jouraiphy, A., Meddich, A., Meddich, A., El Gharous, M., Winterton, P., and Hafid, M., J. Hazard. Mater., 2010, vol. 177, no. 1–3, pp. 524–529. https://doi.org/10.1016/j.jhazmat.2009.12.064

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This study was financially supported by Russian Science Foundation (project no. 20-65-47052).

Author information

Authors and Affiliations

  1. Siberian State Medical University, 634050, Tomsk, Russia

    L. A. Logvinova, M. V. Zykova, S. V. Krivoshchekov, L. A. Drygunova, I. A. Perederina, O. A. Golubina & M. V. Belousov

  2. Moscow State University, 119991, Moscow, Russia

    I. V. Perminova & A. I. Konstantinov

Authors
  1. L. A. Logvinova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. V. Zykova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. V. Krivoshchekov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. A. Drygunova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. A. Perederina
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. O. A. Golubina
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. I. V. Perminova
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. I. Konstantinov
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. M. V. Belousov
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed to manuscript preparation and participated in the discussions.

Corresponding author

Correspondence to M. V. Zykova.

Ethics declarations

This article does not contain any studies involving patients or animals as test objects. Informed consent was not required for this article. No conflict of interest was declared by the authors.

Additional information

Publisher's Note. Pleiades Publishing remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Logvinova, L.A., Zykova, M.V., Krivoshchekov, S.V. et al. A Comparative Pharmacognostic Study of Lowland Wood-Grass Peats of Different Origin for Substantiation of Their Use as Promising Sources of Biologically Active Humic Acids. Russ J Bioorg Chem 49, 1677–1688 (2023). https://doi.org/10.1134/S1068162023070890

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162023070890

Keywords:

Search

Navigation