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Interaction of arenicin-1 with C1q protein

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Abstract

The interaction of arenicin-1, an antimicrobial peptide from the lugworm Arenicola marina with the protein C1q of the human complement system has been analyzed using enzyme-linked receptor sorbent assay and ELISA. Arenicin-1 and C1q were shown to form a stable complex that persisted at elevated ionic strength (0.5 M NaCl). The ability of arenicin-1 to interact with C1q is comparable to that of the porcine cathelicidin protegrin-1, an antimicrobial peptide that has a spatial structure similar to that of arenicin (an antiparallel ß-hairpin stabilized by disulfide bridges).

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Abbreviations

AMPs:

antimicrobial peptides

PBS0:

0.01 M phosphate buffered saline

pH 7.4:

supplemented with 0.15 M NaCl

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Authors and Affiliations

  1. Institute of Experimental Medicine, ul. akad. Pavlova, 12, Saint Petersburg, 197376, Russia

    M. N. Berlov, E. S. Umnyakova, B. L. Milman & V. N. Kokryakov

  2. Saint Petersburg State University, Saint Petersburg, Russia

    M. N. Berlov, T. S. Leonova & V. N. Kokryakov

  3. Center for Infection and Immunity, Queen’s University of Belfast, Belfast, UK

    A. D. Krasnodembskaya

  4. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    T. V. Ovchinnikova

Authors
  1. M. N. Berlov
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  2. E. S. Umnyakova
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  3. T. S. Leonova
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  4. B. L. Milman
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  5. A. D. Krasnodembskaya
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  6. T. V. Ovchinnikova
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  7. V. N. Kokryakov
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Correspondence to M. N. Berlov.

Additional information

Original Russian Text © M.N. Berlov, E.S. Umnyakova, T.S. Leonova, B.L. Milman, A.D. Krasnodembskaya, T.V. Ovchinnikova, V.N. Kokryakov, 2015, published in Bioorganicheskaya Khimiya, 2015, Vol. 41, No. 6, pp. 664–668.

The article has been adapted from the report presented at the VIIth All-Russian symposium “Proteins and Peptides”, Novosibirsk, 12–17 July, 2015.

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Berlov, M.N., Umnyakova, E.S., Leonova, T.S. et al. Interaction of arenicin-1 with C1q protein. Russ J Bioorg Chem 41, 597–601 (2015). https://doi.org/10.1134/S1068162015060035

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  • DOI: https://doi.org/10.1134/S1068162015060035

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