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Changes in the Concentration of Extracellular DNA and Peripheral Blood Leukocytes in the Early Stages of Development of Radiation Cystitis in Rats

  • RADIOBIOLOGICAL FOUNDATIONS OF RADIATION THERAPY OF TUMORS
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Abstract—

The study was designed to identify an index that changes shortly after irradiation and can be used for early (within 6–24 h) diagnosis of the initiation of cystitis as a complication arising during radiation therapy of patients with malignant neoplasms in the pelvic organs. Our newly developed model of radiation-induced cystitis is based on a single irradiation of the urinary bladder area in rats on a linear electron accelerator Axesse (Elekta, Sweden) with a quantum energy of 6 MeV at a dose of 25 Gy at a dose rate of 4.50 Gy/min. Early effects were estimated by a change in the total number of leukocytes and their fractions, the concentration of extracellular DNA (exDNA), and the cholesterol, gamma-glutamyl transferase, urea, and alkaline phosphatase of the serum in the peripheral blood in 6 and 24 h after irradiation. Within 6 h after the effect, a decrease in the total number of leukocytes, lymphocytes, and monocytes was observed (from 16.06 ± 1.83 to 10.57 ± 1.06; from 11.44 ± 1.30 to 5.84 ± 0.47; and from 0.51 ± 0.08 to 0.32 ± 0.03 (×109/L), respectively), while 24 h later, the number of granulocytes also decreased from 4.11 ± 0.57 to 2.14 ± 0.15 (×109/L). In 6 and 24 h after irradiation, there was an increase in the concentration of exDNA in the blood serum from 7.70 ± 0.55 to 11.20 ± 1.33 ng/μL normalized by 48 h after the effect. ExDNA is an index of cell death with a local effect of radiation and can be a promising early noninvasive marker of the development of radiation cystitis, which will probably be important in the choice of means for the prevention of the occurrence of radiation pathology.

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Authors and Affiliations

  1. Petrov National Medical Research Center of Oncology, Ministry of Health of Russian Federation, St. Petersburg, Russia

    I. N. Vasilyeva, S. D. Ivanov, A. L. Semenov & V. G. Bespalov

  2. Granov All-Russia Scientific Center for Radiology and Surgical Technologies, Ministry of Health of the Russian Federation, St. Petersburg, Russia

    O. V. Korytov & L. I. Korytova

Authors
  1. I. N. Vasilyeva
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  2. O. V. Korytov
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  3. S. D. Ivanov
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  4. A. L. Semenov
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  5. V. G. Bespalov
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  6. L. I. Korytova
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Correspondence to I. N. Vasilyeva.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. This work was carried out according to the requirements of current standards for the maintenance and use of laboratory animals.

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Translated by A. Barkhash

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Vasilyeva, I.N., Korytov, O.V., Ivanov, S.D. et al. Changes in the Concentration of Extracellular DNA and Peripheral Blood Leukocytes in the Early Stages of Development of Radiation Cystitis in Rats. Biol Bull Russ Acad Sci 49, 2279–2284 (2022). https://doi.org/10.1134/S106235902212024X

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  • DOI: https://doi.org/10.1134/S106235902212024X

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