Abstract
A variant of the method of differential pulse voltammetry is proposed for the using in the study of a new group of natural antioxidants, polar lipids with paramagnetic properties, in the total lipids of marine organisms. Voltammetric analysis of the total lipids of the Mediterranean mussel Mytilus galloprovincialis L. (gonads) and the Black Sea katran Squalus acanthias (liver) showed them to have one similar anodic wave with an oxidation peak potential of +0.60 V. The difference between the studied hydrobionts manifested itself in the registration of one more anodic wave with the oxidation peak potentials of +1.20 V in the total lipids of the mussel gonad and +0.90 V in those of the katran liver. The using of the proposed version of the method of differential pulse voltammetry together with ESR-spectroscopy and spectrofluorometry in the study of the physico-chemical properties of the new group of polar lipids with paramagnetic properties is substantiated.
Similar content being viewed by others
REFERENCES
Barsukova, M.E., Veselova, I.A., and Shekhovtsova, T.N., Main Methods and Approaches to the Determination of Markers of Oxidative Stress—Organic Peroxide Compounds and Hydrogen Peroxide, J. Anal. Chem., 2019, vol. 74, no. 5, p. 425. https://doi.org/10.1134/S1061934819020035
Khodos, M.Ya., Kazakov, Ya.E., Vidrevich, M.B., and Brainina, Kh.Z., Monitoring of oxidative stress in biological objects, Vestn. Ural. Med. Akad. Nauk. (in Russian), 2017, vol. 14, no. 3, p. 262.]
Pisoschi, A. M., Cimpeanu, C., and Predoi, G., Electrochemical methods for total antioxidant capacity and its main contributor’s determination: A review, Open Chem., 2015, vol. 13(1), p. 824. https://doi.org/10.1515/chem-2015-0099
Kounaves, S.P., Voltammetric Techniques, Ch. 37, Handbook of Instrumental Techniques for Analytical Chemistry, Eds. Frank A. Settle, Upper Saddle River, NJ: Prentice Hall PTR, 1997, p. 709. ISBN-10: 0-13-177338-0.
Henze, G., Polarographie und Voltammetrie. Grundlagen und analytische Praxis, Heidelberg: Springer, 2001. https://doi.org/10.1007/978-3-642-56492-5
Arteaga, J.F., Ruiz-Montoya, M., Palma, A., Alonso-Garrido, G., Pintado, S., and Rodriguez-Mellado, J.M., Comparison of the simple cyclic voltammetry (CV) and DPPH assays for the determination of antioxidant capacity of active principles, Molecules, 2012, vol. 17(5), p. 5126. https://doi.org/10.3390/molecules17055126
Yang, Y., Hu, N., Deng, J., and Yang, J., Electrochemical Sensing for Vitamins, Chemosensors, 2022, vol. 10(11), p. 494. https://doi.org/10.3390/chemosensors10110494
Lovander, M. D., Lyon, J. D., Parr, D. L., Wang, J., Parke, B., & Leddy, J., Critical Review—Electrochemical Properties of 13 Vitamins: A Critical Review and Assessment, J. Electrochem. Soc., 2018, vol. 165(2), p.G18. https://doi.org/10.1149/2.1471714jes
Webster, R. D., Voltammetry of the liposoluble vitamins (A, D, E and K) in organic solvents, The Chemical Record, 2012, vol. 12(1), p. 188. https://doi.org/10.1002/tcr.201100005
Vladimirov, Yu.A. and Archakov, A.I., Lipid Peroxidation in Biological Membranes (in Russian), Moscow: Nauka, 1972.
Webster, R.D., Electrochemical and Spectroscopic Characterization of Oxidized Intermediate Forms of Vitamin E, Molecules, 2022, vol. 27(19), p. 6194. https://doi.org/10.3390/molecules27196194
Uss, Yu.A., Krasheninnikov, B.N., and Minligareev, V.T., Study of the paramagnetic properties of marine organism’s total lipids, Monitoring systems environment (in Russian), 2018, no. 14(34), p. 138.
Uss, Y.A., Krasheninnikov, B.N., and Minligareev, V.T., Fluorescence Excitation Spectra of Total Lipids with Paramagnetic Properties Isolated from Marine Organisms, Doklady Biochemistry Biophysics, 2020, vol. 492, no. 1, p. 159. https://doi.org/10.1134/s1607672920030114
Nekhoroshev, M.V., Uss, Y.A., Klimov, E.S., Berberova, N.T., and Okhlobystin, O.Yu., Stable radical from lipid extracts of marine organisms, Dokl. Acad. Nauk Ukr. SSR, Ser. B: Geol., Chem., Biol. Sci. (in Russian), 1989, no. 10, p. 78.
Folch, J., Lees, M., and Sloane Stanley, G.H., A simple method for the isolation and purification of total lipids from animal tissues, J. Biol. Chem., 1957, vol. 226, p.497.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest.
Additional information
Translated by Yu. Pleskov
Rights and permissions
About this article
Cite this article
Uss, Y.A., Krasheninnikov, B.N. & Minligareev, V.T. Voltammetry of Total Lipids with Paramagnetic Properties from Marine Organisms. Russ J Electrochem 59, 628–633 (2023). https://doi.org/10.1134/S1023193523080104
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1023193523080104