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Homologs of Late Blight Resistance Genes in Representatives of Tuber-Bearing Species of the Genus Solanum L.

  • PLANT GENETICS
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Abstract

The causal agent of late blight, the oomycete Phytophthora infestans Mont de Bary, is characterized by a high degree of variability, as a result of which new races of the pathogen are able to overcome the resistance of long-term cultivated potato cultivars. Primitive cultivated potato species belong to the primary gene pool, the representatives of which easily cross with Solanum tuberosum L., and their use for breeding is promising. The objective of this study was to identify the genotypes of wild and primitive cultivated potato species from the VIR collection carrying the Rpi genes. For the first time, accessions of primitive cultivated and wild potato species (105 genotypes from the VIR collection) were analyzed for resistance to late blight and the presence of SCAR markers of the Rpi genes (RB/blb1, Rpi-blb2, R2-like, Rpi-vnt1.3). In the cultivated species S. stenotomum subsp. stenotomum, high (0.71) frequency of one of the two marker fragments of the RB/blb1 gene (Rpi-sto1), originally characterized in the wild North American species S. bulbocastanum, which belongs to the tertiary gene pool of potato species, was detected. In the species S. phureja and S. stenotomum subsp. goniocalyx, high frequency (0.71–0.88) of the Rpi-vnt1.3 gene marker, originally characterized in the wild South American species S. venturii, was found. For the first time, in primitive potato species, the fragment sequences, presumptive homologs of the Rpi-vnt1 and RB/blb1 genes, were characterized. In S. ajanhuiri, S. stenotomum, and S. phureja, three sequence variants homologous to Rpi-vnt1.3 were identified. The possible role of the detected polymorphism of the Rpi-vnt1.3 marker fragments in ensuring the resistance of primitive cultivated species to late blight is speculated.

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ACKNOWLEDGMENTS

Sequencing of the Rpi gene fragments was carried out using the equipment of the Genomic Technologies, Proteomics, and Cell Biology Resource Center of the All-Russian Research Institute for Agricultural Microbiology, Pushkin, St. Petersburg.

Funding

This study was supported by the Russian Science Foundation, grant no. 22-26-00111 “Potato Late Blight Resistance Genes in the Context of the Evolution of Cultivated and Wild Tuber-Bearing Species of Solanum L.”

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Authors and Affiliations

  1. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000, St. Petersburg, Russia

    A. A. Gurina, N. V. Alpatieva, N. A. Chalaya & E. V. Rogozina

  2. All-Russian Research Institute of Plant Protection, 196608, Pushkin, St. Petersburg, Russia

    N. V. Mironenko & A. V. Khiutti

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  1. A. A. Gurina
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  2. N. V. Alpatieva
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  3. N. A. Chalaya
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  4. N. V. Mironenko
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  6. E. V. Rogozina
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Correspondence to A. A. Gurina.

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Translated by N. Maleeva

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Gurina, A.A., Alpatieva, N.V., Chalaya, N.A. et al. Homologs of Late Blight Resistance Genes in Representatives of Tuber-Bearing Species of the Genus Solanum L.. Russ J Genet 58, 1473–1484 (2022). https://doi.org/10.1134/S1022795422120043

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