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Methodologies for Ancient DNA Extraction from Bones for Genomic Analysis: Approaches and Guidelines

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Abstract

The emergence and development of massive parallel sequencing (new generation sequencing) methods have opened up new prospects in the study of ancient organisms, including extinct ones. Numerous skeletal remains from archaeological and museum collections are often the only source of information on ancient species and populations. In this review, we discuss the features of human bone tissue and the advantages and disadvantages of bone material as a source of DNA for genomic analysis of ancient people. Here we present new methodological approaches to DNA extraction from ancient human skeletal remains and its preparation for large-scale parallel sequencing are presented, as well as prospects and directions for further research in a new interdisciplinary field, paleogenomics.

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ACKNOWLEDGMENTS

We thank M.V. Dobrovolskaya, N.A. Makarov (Institute of Archaeology, Russian Academy of Sciences) and A.P. Buzhilova (Research Institute and Museum of Anthropology, Moscow State University) for providing bone specimens.

Funding

This study was supported by the project of the Russian Ministry of Education and Science, system no. 075-10-2020-116 (grant no. 13.1902.21.0023).

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Authors and Affiliations

  1. Center for Genetics and Genetic Technologies, Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    T. V. Andreeva, A. B. Malyarchuk, A. D. Soshkina & N. A. Dudko

  2. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia

    T. V. Andreeva

  3. Center for Genetics and Life Sciences, Sirius University of Science and Technology, 354340, Sochi, Russia

    T. V. Andreeva, N. A. Dudko, M. Yu. Plotnikova & E. I. Rogaev

  4. Department of Genetics, Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    E. I. Rogaev

  5. Department of Psychiatry, UMass Chan Medical School, MA 01545, Worcester, USA

    E. I. Rogaev

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  1. T. V. Andreeva
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  2. A. B. Malyarchuk
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  3. A. D. Soshkina
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Correspondence to T. V. Andreeva or E. I. Rogaev.

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Translated by N. Maleeva

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Andreeva, T.V., Malyarchuk, A.B., Soshkina, A.D. et al. Methodologies for Ancient DNA Extraction from Bones for Genomic Analysis: Approaches and Guidelines. Russ J Genet 58, 1017–1035 (2022). https://doi.org/10.1134/S1022795422090034

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