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Study on Exploration Potential Anti-Cancer Activity miRNAs and Its Expression Rule Related to Growth Years in Mountain-Cultivated Ginseng

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Abstract

Plant-derived miRNAs exist in mammals in a cross-kingdom manner and many studies have focused on their role in regulating and treating diseases. Mountain-cultivated ginseng (Panax ginseng C.A. Mey.) is a valuable Chinese medicine with anti-cancer activity. However, the miRNAs with anti-cancer activity are less studied. In this study, 12 mountain-cultivated ginseng samples were collected from Liaoning Province, China, at 4, 15, 18, and 20 growth years, three biological replicates of each growth year were performed. Small RNA libraries were constructed and sequenced on the BGISEQ-500 platform. By sequencing analysis, 299 miRNAs were identified, including 48 known miRNAs and 251 potential novel miRNAs. A total of 4633 potential human genes were predicted as putative targets of miRNAs by using RNA hybrid, Miranda, and Target Scan software. Then 75 miRNAs were screened to target 277 cancer genes. Interestingly, pgi-miR6135a targeted SOS1 to inhibit cancer occurrence, and its expression level increased with the growth years. It is suggested that the expression of miRNA may affect the anti-cancer activity of mountain-cultivated ginseng in different growth years. However, the human targets of mountain-cultivated ginseng miRNAs need to be confirmed through further experimental validation. This study predicted the miRNA of mountain-cultivated ginseng with anti-cancer activity and provided insights for researches investigating the efficacy of traditional Chinese medicine from the perspective of cross-kingdom regulation of plant-derived miRNAs.

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ACKNOWLEDGMENTS

The authors are grateful to Beijing University of Chinese Medicine for providing the platform for analysis and the Project “Research Project on Customization and Full Traceability Technology of Precise Mongolian Herbs (ZY20201231, no. 90020271520010)” and the Project “Research on Precise Traceability Standards and Key Technologies for Centralized Procurement of Traditional Chinese Medicine Materials/Slices (no. 2022110031015543)”.

Funding

Research Project on Customization and Full Traceability Technology of Precise Mongolian Herbs (ZY20201231, no. 90020271520010), Research on Precise Traceability Standards and Key Technologies for Centralized Procurement of Traditional Chinese Medicine Materials/Slices (no. 2022110031015543).

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Authors and Affiliations

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

    L. Ding, W. Chen, W. Liu, X. Wang, F. Liu, T. Zhao, S. Wei & Y. Zhang

  2. School of Life and Science, Beijing University of Chinese Medicine, Beijing, China

    Y. Shi

  3. Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China

    X. Wang

  4. Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs of Ministry of Education, Beijing, China

    F. Liu, T. Zhao, S. Wei & Y. Zhang

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  1. L. Ding
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  2. W. Chen
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  3. Y. Shi
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  4. W. Liu
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  5. X. Wang
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  6. F. Liu
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  7. T. Zhao
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  8. S. Wei
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  9. Y. Zhang
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Contributions

All authors contributed to the study conception and design.Conception and design of the work, analysis and interpretation of the data, statistical analysis, drafting the manuscript were performed by Ding Lixue; data collection was performed by Chen Wanjin; critical revision of the manuscriptwas performed by Shi Yue and Liu Wenqing, Wang Xiaohui; samples collectionwas performed by Liu Fengbo and Zhao Ting; design of the studywas performed by Wei Shengli and Zhang Yuan. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to S. Wei or Y. Zhang.

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The authors declare that they have no conflict of interest.

Additional information

Abbreviations: GO—Gene ontology; JAK1—Janus Kinases 1; KEGG—Kyoto Encyclopedia of Genes and Genomes; nt—nucleotides; SOS1—Son of sevenless 1; TCM—traditional Chinese medicine; UMI—unique molecular identifiers.

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Ding, L., Chen, W., Shi, Y. et al. Study on Exploration Potential Anti-Cancer Activity miRNAs and Its Expression Rule Related to Growth Years in Mountain-Cultivated Ginseng. Russ J Plant Physiol 70, 62 (2023). https://doi.org/10.1134/S1021443723600551

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