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Identification of a Regulatory Module (PsMYB12L/PsDFR) with Potential Involved in Double-Color Formation in Paeonia suffruticosa “Shima Nishiki”

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Abstract

Paeonia suffruticosa “Shima Nishiki” is a extremely precious double-color cultivar in the world because of its unique and attractive flower color. However, the underlying molecular mechanisms of its double-color formation have not been completely unravelled until now. In the present study, firstly, the full-length cDNA sequence, genomic DNA sequence, promoter region sequence of the key structural gene (PsDFR) in the red and pink petals of the “Shima Nishiki” cultivar were cloned and analyzed, respectively. Meanwhile, the methylation level of CpG island and promoter region of this gene in the red and pink petals was also measured. Moreover, the identification of regulatory effect of PsMYB114L/PsMYB12L and PsDFR was performed. Here, we found that the full-length cDNA sequence, genomic DNA sequence, promoter region sequence of PsDFR were identical in the red and pink petals. There were some differences for the methylation level of this gene in the red and pink petals, but these differences were little and did not show obvious regularity. Therefore, the differential expression of PsDFR should be not caused by its own sequence differences. In addition, the regulatory effect of PsMYB12L and PsDFR was successfully identified. Based on these above results, we concluded that PsMYB12L regulating the differential expression of PsDFR may be a key reason for the double-color formation. These results will advance our understanding of the molecular regulatory mechanisms of double-color formation in P. suffruticosa “Shima Nishiki”.

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ACKNOWLEDGMENTS

We would like to thank Wenshuang Zhao for his invaluable help and assistance.

Funding

This research was funded by Natural Science Foundation of Shandong Province (ZR2022MC013), Agricultural improved variety project of Shandong Province (2020LZGC011-1) and Doctoral fund project of Shandong Jianzhu University (X20021S0101).

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    Authors and Affiliations

    1. Landscape Architecture Research Center, Shandong Jianzhu University, Jinan, China

      X. P. Zhang & C. Li

    2. State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River, College of Forestry, Shandong Agricultural University, Taian, China

      X. Han, M. Y. Zhao, X. Y. Yu & Z. D. Xu

    3. Qixia Gardening Construction and Maintenance Center, Qixia, China

      S. J. Lu

    4. Shandong Academy of Forestry Sciences, Jinan, China

      Y. F. Fang & J. Q. Chen

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    Contributions

    X.P. Zhang and X. Han have contributed equally to this work and share first authorship. Z.D. Xu and J.Q. Chen conceived and designed the research. X.P. Zhang and X. Han participated in the specific design of the study. X.P. Zhang and X. Han performed the experiments and the data analysis, and drafted the manuscript. M.Y. Zhao, S.J. Lu, X.Y. Yu, Y.F. Fang, C. Li contributed analysis tools and helped analyze the data. All authors contributed to manuscript revision and approved the final version.

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    Abbreviations: ANS—anthocyanidin synthase gene; CHS—chalcone synthase gene; CHI—chalcone isomerase gene; DFR—dihydroflavonol 4-reductase gene; F3H—flavanone 3-hydroxylase gene; F3'H—flavonoid 3'-hydroxylase gene; SN-Pink—pink petal; SN-Red—red petal; TFs—transcription factors.

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    Zhang, X.P., Han, X., Zhao, M.Y. et al. Identification of a Regulatory Module (PsMYB12L/PsDFR) with Potential Involved in Double-Color Formation in Paeonia suffruticosa “Shima Nishiki”. Russ J Plant Physiol 70, 26 (2023). https://doi.org/10.1134/S1021443722601823

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