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Functional Condition of Photosystem II in Leaves of Spring Oats during Autumnal Decrease in Temperature

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Abstract

Dynamics of chlorophyll a fluorescence changes (including JIP test parameters) and photosynthetic pigments were studied in oat (Avena sativa L.) leaves of late summer sowing during autumnal cooling. As the average daily temperature decreased from 8 to 2°С, the chlorophyll content decreased by 40%, while the chlorophyll a/b ratio ranged between 2.2 and 2.4. The total pool of carotenoids and the relative content of β‑carotene, lutein, neoxanthin, and pigments of the violaxanthin cycle changed insignificantly. The relative number of QB-nonreducing reaction centers (RC) of photosystem II (PSII) and the quantum yield of energy-dependent component qE of nonphotochemical quenching in the PSII antenna (φNPQ parameter) were found to increase during the seasonal cooling. The values of Fv/Fm parameter characterizing the maximal photochemical efficiency of PSII complexes were 0.72–0.76. At temperatures around 0°C, Fv/Fm decreased to 0.62. At the same time, the nonregulated (light-independent) thermal dissipation (photoinhibitory component qI of nonphotochemical quenching) was enhanced, which was manifested in the increase in parameters φDo and DI0/RC. It is concluded that, under seasonal cooling, the PSII reaction centers partly lose photochemical activity and start functioning as dissipative centers of excitation energy. In a significant part of inactivated RC, the loss of QA-reduction was reversible, which implies the formation of photoinactivated forms of PSII complexes stabilized at low temperatures. The decrease in daily average temperatures to −3°С with nighttime low temperatures from −7 to −8°С led to plant death within 3 days.

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Funding

The study was supported by the federal budget allocated for the state programs of the Institute for Biological Problems of the Cryolithozone, Yakutsk Research Center, Siberian Branch, Russian Academy of Sciences (no. АААА-А17-117020110054-6) and the Institute of Biology, Komi Research Center, Ural Branch, Russian Academy of Sciences (no. АААА-А17-117033010038-7).

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Authors and Affiliations

  1. Institute for Biological Problems of the Cryolithozone, Siberian Branch, Russian Academy of Sciences, 677000, Yakutsk, Russia

    V. E. Sofronova & V. A. Chepalov

  2. Institute of Biology, Komi Research Center, Ural Branch, Russian Academy of Sciences, 167982, Syktyvkar, Russia

    O. V. Dymova & T. K. Golovko

Authors
  1. V. E. Sofronova
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  2. V. A. Chepalov
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  3. O. V. Dymova
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  4. T. K. Golovko
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Correspondence to V. E. Sofronova.

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This article does not contain any studies with human participants or animals performed by any of the authors. The authors declare that they have no conflicts of interest.

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Translated by A. Bulychev

Abbreviations: Chl―chlorophyll; ETC―electron transport chain; ETR―electron transport rate; LHC―light-harvesting complex; NPQ―nonphotochemical quenching of chlorophyll fluorescence; OJIP―induction curves of chlorophyll fluorescence; PAM―pulse-amplitude-modulation; PAR―photosynthetically active radiation; PPFD―photosynthetic photon flux density; PQ―plastoquinone pool; PSI and PSII―photosystems I and II; PSA―photosynthetic apparatus; ROS—reactive oxygen species; φNPQ―quantum yield of energy-dependent component qE of NPQ that is sensitive to pH-dependent regulation of the violaxanthin cycle.

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Sofronova, V.E., Chepalov, V.A., Dymova, O.V. et al. Functional Condition of Photosystem II in Leaves of Spring Oats during Autumnal Decrease in Temperature. Russ J Plant Physiol 67, 661–670 (2020). https://doi.org/10.1134/S1021443720030206

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