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Characteristics of Chlorophyll Fluorescence and Antioxidant-Oxidant Balance in PEPC and PPDK Transgenic Rice under Aluminum Stress

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Abstract

Aluminum is one of the most important heavy metals inducing stress during plant growth and development. In this study, transgenic rice (Oryza sativa L., cv. Kitaake) plants expressing the maize C4PEPC and PPDK genes were evaluated for aluminum tolerance. A 4.3 and 19.1 folds increase of PPDK and PEPC activities in transgenic rice produced increases in root exudation of oxalate, malate, and citrate (1.20, 1.41, and 1.65 times, respectively) compared to untransformed (WT) plants. Transgenic rice had enhanced aluminum tolerance compared to WT based on chlorophyll fluorescence and chlorophyll levels. Transgenic plants under aluminum stress also had decreased lipid membrane oxidative damage and higher levels of ROS-scavenging enzyme activity. The PEPC and PPDK genes play an important role in aluminum stress tolerance by increasing the effluxes of organic acids.

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Abbreviations

CAT:

catalase

CK:

PEPC and PPDK transgenic rice

MDA:

malondialdehyde

NPQ:

non-photochemical quenching

PEPC:

phosphoenolpyruvate carboxylase

POD:

peroxidase

PPDK:

pyruvate orthophosphate dikinase

PSII:

photosystem II

ΦPSII:

the actual PSII quantum efficiency

ROS:

reactive oxygen species

SOD:

superoxide dismutase

WT:

wild type

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Authors and Affiliations

  1. Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei, 230036, China

    Y. H. Zhang, E. M. Wang, T. F. Zhao & Q. Q. Wang

  2. College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China

    L. J. Chen

Authors
  1. Y. H. Zhang
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  2. E. M. Wang
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  3. T. F. Zhao
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  4. Q. Q. Wang
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  5. L. J. Chen
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Correspondence to Y. H. Zhang.

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Zhang, Y.H., Wang, E.M., Zhao, T.F. et al. Characteristics of Chlorophyll Fluorescence and Antioxidant-Oxidant Balance in PEPC and PPDK Transgenic Rice under Aluminum Stress. Russ J Plant Physiol 65, 49–56 (2018). https://doi.org/10.1134/S1021443718010211

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  • DOI: https://doi.org/10.1134/S1021443718010211

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