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Phylogenetic position of the purple sulfur bacterium Lamprobacter modestohalophilus determined based on the data on new strains of the species

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Abstract

Lamprobacter, the genus of halophilic purple sulfur bacteria (PSB) with the single species Lpb. modestohalophilus was described in 1979. Rod-shaped Lamprobacter cells contained gas vesicles during the nonmotile growth phase; motile cells without gas vesicles were sometimes formed. Bacteria contained bacteriochlorophyll a and a carotenoid okenone. The names of this genus and species were included in the list of approved microbial names in 1988. Since the type strain Lpb. modestohalophilus RO1T has been lost, its 16S rRNA gene sequences have not been obtained. Based on analysis of the 16S rRNA genes, a new genus Halochromatium comprising the motile extremely halophilic Chromatium-like species was proposed in 1998. Members of this genus never contain gas vesicles. In spite of the phenotypic differences between the genera Lamprobacter and Halochromatium, phylogenetic boundaries between these taxa remained undetermined. Description of a marine bacteria formerly belonging to Lamprobacter according to its morphological and physiological properties as a new Halochromatium species, Hch. roseum, resulted in additional complication of the taxonomic situation. The present work provides evidence for the preservation of two phenotypically and phylogenetically different genera, Lamprobacter and Halochromatium, Lpb. modestohalophilus is proposed as the type species of the genus Lamprobacter. Characteristics of two Lpb. modestohalophilus strains were extensively investigated, and one of them (strain Sivash) was proposed as the neotype strain of the species. It was suggested to retain the genus Halochromatium as containing extremely halophilic species Hch. salexigens and Hch. glycolicum, while transfer of the weakly halophilic species Hch. roseum to the genus Lamprobacter is proposed, resulting in a new combination Lamprobacter roseus comb. nov.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    V. M. Gorlenko, I. A. Bryantseva, O. N. Lunina & T. P. Tourova

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  1. V. M. Gorlenko
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  2. I. A. Bryantseva
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  3. O. N. Lunina
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  4. T. P. Tourova
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Correspondence to V. M. Gorlenko.

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Gorlenko, V.M., Bryantseva, I.A., Lunina, O.N. et al. Phylogenetic position of the purple sulfur bacterium Lamprobacter modestohalophilus determined based on the data on new strains of the species. Microbiology 83, 829–837 (2014). https://doi.org/10.1134/S0026261714060071

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