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EPR study of nitric oxide production in rat tissues under hypokinesia

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Abstract

EPR spectroscopy was used to study the intensity of nitric oxide (NO) production upon modeling 60-day progressive hypokinesia (restriction of motor activity) in rats and estimating the content of (DETC)2-Fe2+-NO complexes in heart and liver tissues. In 30 days of hypokinesia, there was a 2–3-fold increase in tissue NO. Administration of a nonspecific inhibitor of NO synthases, L-NAME, to hypokinetic rats prior to measurement decreased their NO level even below the untreated control. Our results show that the intensified NO production in hypokinesia is mainly due to NO synthases, rather than to the nitrite reductase pathway.

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Correspondence to Kh. L. Gainutdinov.

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Original Russian Text © Kh.L. Gainutdinov, V.V. Andrianov, V.S. Iyudin, S.V. Yurtaeva, G.G. Jafarova, R.I. Faisullina, F.G. Sitdikov, 2013, published in Biofizika, 2013, Vol. 58, No. 2, pp. 276–280.

The experimental data contained herein fully correspond to the original publication but the presentation had to be substantially revised for the English version. A.G.

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Gainutdinov, K.L., Andrianov, V.V., Iyudin, V.S. et al. EPR study of nitric oxide production in rat tissues under hypokinesia. BIOPHYSICS 58, 203–205 (2013). https://doi.org/10.1134/S0006350913020073

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  • DOI: https://doi.org/10.1134/S0006350913020073

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