Skip to main content
SpringerLink
Log in

Properties of Extracellular Protease—Regulator of Hemostasis Produced by Micromycete Aspergillus tabacinus

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

The extracellular protease with protein C-like and plasmin-like activities was isolated from the culture fluid of the micromycete A. tabacinus BEOFB3260m. It has been established that A. tabacinus extracellular protease is a non-glycosylated serine protease with molecular weight about 30 kDa. The enzyme is active and stable at 25–37°C, active at pH 7.0–12.0 and stable at pH 3.0–12.0 and is a promising candidate for the development of new anticoagulant drugs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

REFERENCES

  1. Li, T., Yuan, D., and Yuan, J., Adv. Exp. Med. Biol., 2020, vol. 1177, pp. 101–131.

    Article  CAS  PubMed  Google Scholar 

  2. Barzkar, N., Jahromi, S.T., and Vianello, F., Mar. Drugs, 2022, vol. 20, no. 1, p. 46. https://doi.org/10.3390/md20010046

  3. Zhang, S., Wang, Y., Zhang, N., Sun, Z., Shi, Y., Cao, X., and Wang, H., Food Technol. Biotechnol., 2015, vol. 53, no. 2, pp. 243–248.

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Duan, Y., Katrolia, P., Zhong, A., and Kopparapu, N.K., Prep. Biochem. Biotechnol., 2022, vol. 52, no. 9, pp. 1008–1018.

    Article  CAS  PubMed  Google Scholar 

  5. Zhao, L., Lin, X., Fu, J., Zhamg, J., Tang, W., and He, Z., Mar. Drugs, 2022, vol. 20, no. 6, p. 352. https://doi.org/10.3390/md20060356

    Article  Google Scholar 

  6. Batomunkueva, B.P. and Egorov, N.S., Microbiology (Moscow), 2001, vol. 70, no. 5, pp. 519–522.

    Article  CAS  Google Scholar 

  7. Osmolovskiy, A.A., Zvonareva, E.S., Kreyer, V.G., Baranova, N.A., and Egorov, N.S., Russ. J. Bioorg. Chem., 2014, vol. 40, no. 6, pp. 634–639.

    Article  CAS  Google Scholar 

  8. Zvonareva, E.S., Osmolovskiy, A.A., Kreyer, V.G., Baranova, N.A., Kotova, I.B., and Egorov, N.S., Russ. J. Bioorg. Chem., 2015, vol. 41, no. 5, pp. 500–505.

    Article  CAS  Google Scholar 

  9. Laemly, U.K., Nature, 1970, vol. 227, no. 5259, pp. 680–685.

    Article  Google Scholar 

  10. Hu, Y., Yu, D., Wang, Z., Hou, J., Tyagi, R., Liang, Y., and Hu, Y., Sci. Rep., 2019, vol. 9, no. 9235. https://doi.org/10.1038/s41598-019-45686-y

  11. Thornton, D.J., Carlstedt, I., and Sheehan, J.K., Methods Mol. Biol., 1994, vol. 32, pp. 119–128.

    CAS  PubMed  Google Scholar 

  12. Zvonareva, E.S., Osmolovskiy, A.A., Kreyer, V.G., Baranova, N.A., Kotova, I.B., and Egorov, N.S., Appl. Biochem. Microbiol., 2018, vol. 54, no. 2, pp. 206–210.

    Article  CAS  Google Scholar 

  13. Proteolytic Enzymes. A Practical Approach, Beynon, R. and Bond, J.S., Eds., Oxford: Oxford Univ. Press, 2001.

    Google Scholar 

  14. Biaggio, R.T., Silva, R.R., Rosa, N.G., Leite, R.S., Arantes, E.C., Cabral, T.P., Juliano, M.A., Juliano, L., and Cabral, H., Prep. Biochem. Biotechnol., 2016, vol. 46, no. 3, pp. 298–304.

    Article  CAS  PubMed  Google Scholar 

  15. Osmolovskiy, A.A., Kreyer, V.G., Baranova, N.A., Kurakov, A.V., and Egorov, N.S., Appl. Biochem. Microbiol., 2015, vol. 51, no. 1, pp. 95–101.

    Article  CAS  Google Scholar 

  16. Das, A.K., Bellizzi, J.J., Tandel, S., Biehl, E., Clardy, J., and Hofmann, S.L., J. Biol. Chem., 2000, vol. 275, no. 31, pp. 23847–23851.

    Article  CAS  PubMed  Google Scholar 

  17. Richmond, G.S. and Smith, T.K., Biochem. J., 2007, vol. 405, no. 2, pp. 319–329.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Chakrabarti, S.K., Matsumura, N., and Ranu, R.S., Curr. Microbiol., 2000, vol. 40, no. 4, pp. 239–244.

    Article  CAS  PubMed  Google Scholar 

  19. Zvonareva, E.S., Osmolovskii, A.A., Baranova, N.A., Kotova, I.B., and Kreier, V.G., Appl. Biochem. Microbiol., 2023, vol. 59, no. 4, pp. 476–481.

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by ongoing institutional funding. No additional grants to carry out or direct this particular research were obtained.

Author information

Authors and Affiliations

  1. Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    V. N. Lavrenova, V. G. Kreyer & A. A. Osmolovskiy

  2. Faculty of Biology, University of Belgrade, Belgrade, Republic of Serbia

    Z. Savkovic

Authors
  1. V. N. Lavrenova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. G. Kreyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Z. Savkovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Osmolovskiy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. N. Lavrenova.

Ethics declarations

CONFLICT OF INTEREST

The authors of this work declare that they have no conflicts of interest.

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

This work does not contain any studies involving human and animal subjects.

Additional information

Translated by E. V. Makeeva

Publisher’s Note.

Pleiades Publishing remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lavrenova, V.N., Kreyer, V.G., Savkovic, Z. et al. Properties of Extracellular Protease—Regulator of Hemostasis Produced by Micromycete Aspergillus tabacinus. Appl Biochem Microbiol 60, 118–123 (2024). https://doi.org/10.1134/S0003683824010101

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683824010101

Keywords:

Search

Navigation