Abstract
It was shown that the presence of cellobiohydrolase, β-glucosidase, endoglucanase, arabinoxylan-arabinofuranhydrolase, pectin lyase, and polygalacturonase is necessary for efficient hydrolysis of sugar beet pulp (SBP). Optimal multienzyme complexes consisting of the same enzymes and additional endoarabinase or exoarabinase, endogalactanase, β-xylosidase, endoxylanase, and/or α-arabinofuranosidase were determined. These components enabled the conversion of SBP based on a yield of total reducing sugars (RSs) of 61–68%, an arabinose yield of 94%, and a glucose yield of 63–79%. The optimal complex from dry multienzyme preparations (EP) of cellulases, hemicellulases, and pectinases, which are produced by the fungal strains Penicillium canescens, P. verruculosum, and Aspergillus foetidus, enabled SBP hydrolysis based on arabinose and glucose yields close to 100% at an initial SBP concentration of 100–250 g/L and an EP concentration of 5–10 mg protein/g SBP after 24–48 h of hydrolysis.
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Funding
This study was carried out with partial financial support from the Russian Foundation for Basic Research (project no. 18-54-80027). The scientific equipment of the Industrial Biotechnologies Common Use Center and the Bioengineering Association of Common Use Centers of the Biotechnology Federal Research Center of the Russian Academy of Sciences were used in the study.
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Semenova, M.V., Rozhkova, A.M., Osipov, D.O. et al. Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion. Appl Biochem Microbiol 55, 677–683 (2019). https://doi.org/10.1134/S0003683819050119
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DOI: https://doi.org/10.1134/S0003683819050119