Skip to main content
SpringerLink
Log in

Extracellular proteolytic activity of bacteria from soda-salt lakes of Transbaikalia

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

Influence of nitrogen source on proteinases synthesis in aerobic alkalotolerant and halotolerant bacteria from soda-salt lakes of Transbaikalia was studied. Maximal accumulation of proteinases was revealed on medium with peptones. Introduction of various sources of nitrogen in the medium did not result in increase of enzyme activity in cultural liquid. It was indicated that secreting proteinases of the studied bacteria strains possess narrow substrate specificity, hydrolyze proteins and n-nitroanilide substrates have maximal activity during GlpAALpNA hydrolysis. Data of inhibitory analysis and substrate specificity of studied extracellular enzymes indicate that they belong to a class of serine proteinases of subtilisin-like type.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sadler, M., McAninch, M., Alico, R., and Hochstein, L.I., Can. J. Microbiol., 1980, vol. 26, no. 4, pp. 496–502.

    Article  CAS  PubMed  Google Scholar 

  2. Vreeland, R.H., Litchfield, C.D., Martin, E.L., and Elliot, E., Int. J. Syst. Bacteriol., 1980, vol. 30, pp. 485–495.

    Article  CAS  Google Scholar 

  3. Zavarzin, G.A., Trudy Inst. Mikrobiol. im. S.N. Vinogradskogo, 2007, no. 14, pp. 1–8.

  4. Rao, M.B., Tanksale, A.M., Ghatge, M.S., and Deshpande, V.V., Microbiol. Mol. Biol. Rev., 1998, vol. 62, no. 3, pp. 597–635.

    CAS  PubMed  Google Scholar 

  5. Gupta, R., Beg, Q., and Lorenz, P., Appl. Microbiol. Biotechnol., 2002, vol. 59, no. 1, pp. 15–32.

    Article  CAS  PubMed  Google Scholar 

  6. Mitypova, T.N., Kozyreva, L.P., and Namsaraev, B.B., Vestn. Buryat. Univ., Ser. 2: Biol., 2005, no. 7, pp. 190–193.

  7. Dawson, R., Elliott, D., Elliott, W., and Jones, K., Data for Biochemical Research, Oxford: Clarendon, 1986.

    Google Scholar 

  8. Erlanger, B., Kokowsky, N., and Cohen, W., Arch. Biochem. Biophys., 1961, vol. 95, pp. 271–278.

    Article  CAS  PubMed  Google Scholar 

  9. Dunaevskii, Ya.E., Belyakova, G.A., Pavlyukova, E.B., and Belozerskii, M.A., Mikrobiologiya, 1995, vol. 64, no. 3, pp. 327–330.

    CAS  Google Scholar 

  10. Dunaevsky, Y.E., Golubeva, E.A., Gruban, T.N., Beliakova, G.A., and Belozersky, M.A., J. Russ. Phytopathol. Soc., 2001, vol. 2, pp. 39–43.

    Google Scholar 

  11. Braga, G.U.L., Destefano, R.H.R., and Messias, C.L., Revista Microbiol., 1999, vol. 30, no. 2, pp. 107–113.

    CAS  Google Scholar 

  12. Valueva, T.A. and Mosolov, V.V., Usp. Biol. Khim., 2002, vol. 42, pp. 193–216.

    CAS  Google Scholar 

  13. Eggers, C.T., Murray, I.A., Delmar, V.A., Day, A.G., and Craik, C.S., Biochem. J., 2004, vol. 379, no. 1, pp. 107–118.

    Article  CAS  PubMed  Google Scholar 

  14. Kang, S., Barak, Y., Lamed, R., Bayer, E.A., and Morrison, M., Mol. Microbiol., 2006, vol. 10, pp. 1344–1354.

    Article  Google Scholar 

  15. Schwarz, W.H. and Zverlov, V.V., Mol. Microbiol., 2006, vol. 10, no. 1, pp. 1–4.

    Google Scholar 

  16. Singh, J., Batra, N., and Sobti, C., Proc. Biochem., 2001, vol. 36, pp. 781–785.

    Article  CAS  Google Scholar 

  17. Denizci, A.A., Kazan, D., Abeln, E.C.A., and Erarslan, A., J. Appl. Microbiol., 2004, vol. 96, no. 2, pp. 320–327.

    Article  CAS  PubMed  Google Scholar 

  18. Shiga, Y., Yamagata, H., Tsukagoshi, N., and Udaka, S., Biosci. Biotechnol. Biochem., 1995, vol. 59, no. 12, pp. 2348–2350.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, Ulan-Ude, 670057, Russia

    E. V. Lavrenteva, L. P. Kozyreva, A. A. Radnagurueva & B. B. Namsaraev

  2. Belozersky Institute of Physicochemical Biology, Moscow State University, Moscow, 119991, Russia

    Ya. E. Dunaevsky

Authors
  1. E. V. Lavrenteva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ya. E. Dunaevsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. P. Kozyreva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Radnagurueva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. B. Namsaraev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. V. Lavrenteva.

Additional information

Original Russian Text © E.V. Lavrenteva, Ya.E. Dunaevsky, L.P. Kozyreva, A.A. Radnagurueva, B.B. Namsaraev, 2010, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2010, Vol. 46, No. 6, pp. 630–636.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lavrenteva, E.V., Dunaevsky, Y.E., Kozyreva, L.P. et al. Extracellular proteolytic activity of bacteria from soda-salt lakes of Transbaikalia. Appl Biochem Microbiol 46, 580–585 (2010). https://doi.org/10.1134/S0003683810060049

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683810060049

Keywords

Search

Navigation